18 Questions
Which of the following is NOT a structural form of DNA?
C-DNA
What is the approximate distance between stacked bases in the B form of DNA?
0.34 nm
What is the number of hydrogen bonds in complementary base pairings in DNA?
2
Which process can generate positive supercoils that must be relaxed for proper chromosome segregation?
Mitosis and Meiosis
Which of the following is NOT a target of antibiotics used to treat bacterial infections?
Topoisomerases
What stabilizes the double helix of DNA vertically?
Base stacking interactions
What is the temperature at the midpoint of the transition during DNA denaturation?
Tm
Which type of supercoil is generated when the DNA strands are separated at specific sites in a circular chromosome during processes such as DNA replication and transcription?
Negative supercoil
Which type of topoisomerase cleaves both DNA strands, passes intact DNA through the gap, and reseals both DNA strands?
Type II topoisomerase
What is the functional significance of negative supercoiling in genomic DNA?
Compaction of DNA with reduced torsional stress
What is the role of RNA polymerases in DNA transcription?
Unwind DNA to form a negative supercoil
Chargaff's rule states that the content of A is equal to the content of T, and the content of G is equal to the content of C. How can this rule be used to infer the base composition of a given molecule of DNA?
By analyzing the sequence of nucleotides in the molecule
What is the relationship between Linking Number (Lk) and positive versus negative supercoiling?
Positive supercoiling increases Lk, while negative supercoiling decreases Lk
What are the effects of Type I and Type II Topoisomerases on Linking Number (Lk)?
Type I Topoisomerases decrease Lk, while Type II Topoisomerases increase Lk
What is the functional significance of DNA supercoiling and what is the role of Type I and Type II Topoisomerases?
DNA supercoiling helps stabilize the DNA double helix, and Type I Topoisomerases relieve positive supercoiling while Type II Topoisomerases relieve negative supercoiling
Which of the following is NOT a physical property of DNA?
Increased absorbance of UV light
What is the function of gyrase inhibitors in medicine?
To treat Bacillus anthracis infections
What is the role of denaturation and reannealing in research and clinical medicine?
To detect sequences in fragments on Southern blots
Test your knowledge on DNA supercoiling and its role in processes such as replication and transcription. This quiz will cover topics such as positive and negative supercoils, strand separation, and the importance of DNA supercoiling in chromosomes.
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