DNA Structure: 5' and 3' Ends
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Questions and Answers

What defines the 5' end of a DNA strand?

  • It features a phosphodiester linkage.
  • It has a free 5' phosphate group. (correct)
  • It has a free 3' hydroxyl group.
  • It is located at the center of the double helix.
  • What component primarily forms the backbone of DNA's primary structure?

  • A linear strand of sugar phosphate residues. (correct)
  • Deoxynucleoside 5'-monophosphates.
  • A pair of oligomeric nucleotides.
  • Hydrophobic bases stacked together.
  • Which statement accurately describes the structure of DNA?

  • DNA is comprised of two strands of opposite polarity. (correct)
  • DNA bases are located on the exterior of the double helix.
  • DNA has a left-handed helical structure.
  • DNA consists of only one polydeoxyribonucleotide strand.
  • Which unit is not a component of the monomeric units in DNA strands?

    <p>Deoxyribonucleotide (dNTP)</p> Signup and view all the answers

    What characteristic of deoxyribose subunits and phosphate groups is highlighted in DNA's structure?

    <p>They are hydrophilic and face the aqueous environment.</p> Signup and view all the answers

    Which feature of DNA's secondary structure pertains to base arrangement?

    <p>Planar bases are stacked in the interior of the helix.</p> Signup and view all the answers

    Study Notes

    The 5' and 3' Ends

    • The 5' end features a free phosphate group, indicating an unlinked position in the phosphodiester bond.
    • The 3' end is identified by a free hydroxyl group or phosphate group.

    The Primary Structure of DNA

    • Primary structure focuses on the sequence and quantity of deoxyribonucleotides in DNA strands, linked through phosphodiester bonds.
    • The backbone consists of a linear arrangement of sugar-phosphate groups, with nitrogenous bases (purines or pyrimidines) extending from the sugar, creating a lateral projection.

    The Double Helix

    • DNA consists of two complementary strands of polydeoxyribonucleotides with opposing polarity, forming a right-handed helical shape.
    • Each strand is made up of monomeric units known as deoxynucleoside 5'-monophosphates, including dAMP (deoxyadenylate), dGMP (deoxyguanylate), dTMP (deoxythymidine), and dCMP (deoxycytidine).
    • The exterior of the double helix contains hydrophilic deoxyribose sugar and phosphate, interacting with the surrounding aqueous environment, while the hydrophobic interior holds the stacked planar bases.

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    Description

    Learn about the 5' and 3' ends of a DNA strand, including the phosphate groups and hydroxyl groups that define them, as well as the primary structure of DNA.

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