DNA Replication Quiz
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Questions and Answers

What process refers to the duplication of DNA before cell division?

  • Mutation
  • Transcription
  • Translation
  • Replication (correct)

Which enzyme is responsible for adding new bases to the growing daughter strand during DNA replication?

  • Helicase
  • Telomerase
  • DNA Polymerase (correct)
  • DNA Ligase

What term describes the nature of DNA replication where half of the original molecule is retained in each daughter molecule?

  • Semi-conservative (correct)
  • Conservative
  • Dispersive
  • Linear

Which structure is particularly difficult to replicate, causing chromosomes to shorten with each cell division?

<p>Telomeres (C)</p> Signup and view all the answers

During DNA replication, which direction do the sugar-phosphate backbones run?

<p>One runs from 5' to 3' and the other from 3' to 5'. (C)</p> Signup and view all the answers

What is the composition of a nucleotide in DNA?

<p>A sugar, a nitrogenous base, and a phosphate group (B)</p> Signup and view all the answers

Which nitrogenous bases are classified as purines?

<p>Adenine and Guanine (B)</p> Signup and view all the answers

What discovery was made by Erwin Chargaff regarding DNA base pairing?

<p>Adenine equals Thymine and Guanine equals Cytosine (C)</p> Signup and view all the answers

How did Rosalind Franklin contribute to the understanding of DNA's structure?

<p>She used X-ray diffraction to reveal the helical shape of DNA (B)</p> Signup and view all the answers

What allows the two strands of DNA to separate easily?

<p>The presence of weak hydrogen bonds between base pairs (C)</p> Signup and view all the answers

What was the primary goal of Oswald Avery's research?

<p>To determine which molecule in heat-killed bacteria was responsible for transformation. (A)</p> Signup and view all the answers

Which statement accurately describes transformation in bacterial cells?

<p>It occurs when DNA is destroyed in the mixture, resulting in permanent changes in bacteria. (A)</p> Signup and view all the answers

What is one of the essential functions of DNA according to the content?

<p>Storing genetic information. (C)</p> Signup and view all the answers

Which of the following is NOT a step in the life cycle of a phage as described?

<p>The phage injects proteins into the bacterial cell. (A)</p> Signup and view all the answers

Who performed critical experiments in the study of viruses that built upon Avery's research?

<p>Alfred Hershey and Martha Chase. (A)</p> Signup and view all the answers

What is the primary role of RNA in protein synthesis?

<p>To mediate the process of transcription and translation (C)</p> Signup and view all the answers

Which statement accurately describes a difference between DNA and RNA?

<p>RNA contains uracil in place of thymine, which is found in DNA. (B)</p> Signup and view all the answers

What are exons in the context of RNA?

<p>Remaining sections of RNA that are spliced together after introns are removed (B)</p> Signup and view all the answers

Where does transcription occur in prokaryotic cells?

<p>In the cytoplasm (A)</p> Signup and view all the answers

What is the function of a promoter in RNA synthesis?

<p>To indicate where RNA polymerase should bind to initiate transcription (B)</p> Signup and view all the answers

What is the role of tRNA in protein synthesis?

<p>To transfer amino acids to the correct RNA codon (C)</p> Signup and view all the answers

How many codons are needed to specify 4 amino acids?

<p>4 (C)</p> Signup and view all the answers

Which component of a nucleotide differs between DNA and RNA?

<p>The type of sugar (C)</p> Signup and view all the answers

What is the function of rRNA in the cell?

<p>To serve as a structural component of ribosomes (B)</p> Signup and view all the answers

Which of the following statements is true regarding codons?

<p>Only one codon specifies a particular amino acid (A)</p> Signup and view all the answers

What is the primary role of helicase during DNA replication?

<p>To unzip the double helix structure (D)</p> Signup and view all the answers

Which enzyme is responsible for connecting DNA fragments during replication?

<p>Ligase (A)</p> Signup and view all the answers

What is the correct order of processes in protein synthesis?

<p>Transcription followed by translation (B)</p> Signup and view all the answers

What is the role of homeotic genes in body development?

<p>They control the identity of specific organs in the body. (C)</p> Signup and view all the answers

What is the primary role of tRNA during the initiation phase of protein synthesis?

<p>To transfer amino acids to the ribosome (A)</p> Signup and view all the answers

Which type of mutation results in a change of an amino acid to a stop codon?

<p>Nonsense mutation (B)</p> Signup and view all the answers

What kind of bond connects amino acids in a protein chain?

<p>Peptide bonds (A)</p> Signup and view all the answers

Which type of mutation shifts the reading frame of the genetic message?

<p>Frameshift mutation (A)</p> Signup and view all the answers

What occurs during the elongation phase of translation?

<p>tRNA recognizes codons through complementary base pairing (A)</p> Signup and view all the answers

What type of bonds hold the nucleotides together in the structure of nucleic acids?

<p>Covalent bonds (B)</p> Signup and view all the answers

Which component of protein synthesis functions as a stop signal?

<p>Stop codons (A)</p> Signup and view all the answers

What best describes spontaneous mutations?

<p>Errors that occur during DNA replication or recombination. (C)</p> Signup and view all the answers

What is the first step in the process of gene expression?

<p>Transcription of DNA into RNA (C)</p> Signup and view all the answers

What role do transcription factors play in gene expression?

<p>They regulate the expression of genes in eukaryotes (B)</p> Signup and view all the answers

Flashcards

What did Frederick Griffith discover?

Frederick Griffith discovered that a chemical substance within bacteria could be transferred from one bacterium to another, causing a change in its characteristics. This discovery hinted at the nature of genetic material.

What was Avery's group trying to find?

Oswald Avery and his team aimed to isolate the specific molecule responsible for bacterial transformation. They wanted to determine whether DNA, RNA, or protein was the carrier of genetic information.

What is a phage?

A phage is a virus that infects bacteria. It consists of a protein coat surrounding genetic material (DNA or RNA) and uses the host bacterium's machinery to replicate itself.

What are the functions of DNA?

DNA is responsible for storing, copying, and transmitting genetic information. This includes instructions defining an organism's characteristics and ensuring their accurate transfer to offspring.

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What is transformation in bacteria?

Transformation is the process where bacteria permanently changes its characteristics by acquiring genetic material from another bacterium, often through the uptake of DNA fragments.

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What are the building blocks of DNA?

Nucleotides are the building blocks of DNA. Each nucleotide consists of a nitrogenous base, a sugar molecule, and a phosphate group.

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What are the four nitrogenous bases in DNA?

The four nitrogenous bases in DNA are adenine (A), guanine (G), cytosine (C), and thymine (T).

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What is the difference between purines and pyrimidines?

Purines (Adenine and Guanine) have larger, double-ring structures, while pyrimidines (Cytosine and Thymine) have smaller, single-ring structures.

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What is Chargaff's Rule?

Chargaff's Rule states that the amount of adenine (A) in DNA is always equal to the amount of thymine (T), and the amount of guanine (G) is always equal to the amount of cytosine (C).

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What is the structure of DNA?

DNA has a double helix structure, with two strands of nucleotides running in opposite directions (antiparallel) and held together by hydrogen bonds between the bases.

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DNA Replication

The process of copying a DNA molecule into two identical daughter molecules. This is essential for cell division, as it ensures each daughter cell receives a complete set of genetic instructions.

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Semi-Conservative Replication

During DNA replication, each new DNA molecule consists of one parental strand and one newly synthesized strand. This means half of the original molecule is conserved in each daughter molecule.

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DNA Polymerase

An enzyme that adds new nucleotides to a growing DNA strand, following the base pairing rules. It's responsible for building the new DNA molecule during replication.

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DNA Ligase

An enzyme that joins the fragments of DNA into a continuous strand. It's like the glue that holds the newly synthesized DNA together.

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Telomeres

Protective caps at the ends of chromosomes. These regions are difficult to replicate completely, causing them to shorten slightly with each cell division.

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What is RNA transcription?

RNA transcription is the process where DNA's genetic information is copied into RNA. It's like making a photocopy of the blueprint.

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Name three key differences between DNA and RNA.

  1. RNA uses ribose sugar, while DNA uses deoxyribose. 2. RNA is usually single-stranded, while DNA is double-stranded. 3. RNA has uracil instead of thymine found in DNA.
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What are the three main types of RNA?

Messenger RNA (mRNA) carries genetic information from DNA to ribosomes. Ribosomal RNA (rRNA) forms part of the ribosomes, which are protein factories. Transfer RNA (tRNA) brings specific amino acids to the ribosomes for protein assembly.

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What is the role of promoters in RNA transcription?

Promoters are specific DNA sequences that signal where RNA polymerase should bind to begin RNA synthesis. They act like start buttons for the copying process.

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What is the difference between introns and exons?

Introns are non-coding RNA sections removed during RNA editing. Exons are the remaining coding sections spliced together to form mature mRNA.

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What is a codon?

A codon is a sequence of three consecutive nucleotides in mRNA that codes for a specific amino acid. It's like a three-letter word in the genetic code dictionary.

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How many codons are needed for 4 amino acids?

You would need 12 nucleotides in total, forming 4 codons. Each codon requires 3 nucleotides, so 4 amino acids need 4 * 3 = 12 nucleotides.

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What is the genetic code?

The genetic code is a set of rules used by living cells to translate the information in DNA and RNA into proteins. It's like a universal language for protein synthesis.

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What is mRNA's role?

mRNA carries genetic information from DNA in the nucleus to ribosomes in the cytoplasm, where it directs the synthesis of proteins.

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What is tRNA's role?

tRNA acts as a translator, carrying specific amino acids to the ribosome and matching them to the corresponding mRNA codon.

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What is the role of Helicase in DNA replication?

Helicase is an enzyme that unwinds and separates the two strands of the DNA double helix by breaking the hydrogen bonds between the nitrogenous base pairs, creating a replication fork.

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What is the function of DNA Polymerase in DNA replication?

DNA polymerase is an enzyme that reads the existing DNA strand and synthesizes a new complementary strand by adding nucleotides to the growing DNA chain, ensuring the correct base pairing.

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Why is Primase essential in DNA replication?

Primase is an enzyme that synthesizes a short RNA primer, providing a starting point for DNA polymerase to begin adding nucleotides, as DNA polymerase cannot initiate a new strand on its own.

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What is the function of Ligase in DNA replication?

Ligase is an enzyme that joins the fragments of DNA, called Okazaki fragments, together to form a continuous strand after DNA polymerase has replicated the lagging strand.

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What is the difference between transcription and translation?

Transcription is the process of copying the genetic code from DNA into mRNA (messenger RNA), while translation is the process of using the mRNA code to build a protein from amino acids.

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Anticodon

A three-nucleotide sequence on tRNA that binds to a complementary codon on mRNA during translation, bringing the correct amino acid to the growing polypeptide chain.

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Codon

A three-nucleotide sequence on mRNA that codes for a specific amino acid during translation.

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Homeotic Genes

Genes that control the development of specific body structures in particular locations along the body axis.

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Hox Genes

A type of homeotic gene that specifies the identity of different body segments during embryonic development.

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Gene Mutations

Permanent alterations in the DNA sequence of a gene, which can lead to changes in protein function.

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What is the role of tRNA in protein synthesis?

tRNA acts as a carrier for amino acids, bringing them to the ribosome to be added to the growing polypeptide chain. It recognizes specific mRNA codons through base pairing with its anticodon, ensuring the correct amino acid is incorporated into the protein.

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How does the anticodon on tRNA contribute to protein synthesis?

The anticodon on tRNA is a sequence of three nucleotides that binds to a complementary codon on mRNA through base pairing rules. This interaction ensures that the correct amino acid is brought to the ribosome for protein synthesis.

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What is the function of stop codons in translation?

Stop codons are special codons within mRNA that do not code for any amino acid. Instead, they signal the termination of translation, causing the ribosome to release the newly synthesized protein.

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What happens during the elongation phase of translation?

Elongation involves the sequential addition of amino acids to the growing polypeptide chain. tRNA molecules, carrying their respective amino acids, bind to the mRNA codons in the ribosome. The polypeptide chain lengthens as the ribosome moves along the mRNA.

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What is the role of ribosomes in translation?

Ribosomes act as the protein synthesis machines. They bind to mRNA and provide a platform for the tRNA molecules to bring amino acids and assemble them into a polypeptide chain. During translation, the ribosome moves along the mRNA, reading the codons one by one.

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