Podcast
Questions and Answers
What is the function of the primase complex during DNA replication?
What is the function of the primase complex during DNA replication?
- To unwind the double helix
- To join Okazaki fragments together
- To synthesize RNA primers for both leading and lagging strands (correct)
- To synthesize the leading strand
What is the main function of DNA polymerase-δ during lagging strand synthesis?
What is the main function of DNA polymerase-δ during lagging strand synthesis?
- To synthesize the leading strand
- To polymerize the lagging strand (correct)
- To unwind the double helix
- To remove RNA primers from the newly synthesized strand
What is the purpose of ribonucleases during DNA replication?
What is the purpose of ribonucleases during DNA replication?
- To remove RNA primers from the newly synthesized strand (correct)
- To polymerize the lagging strand
- To unwind the double helix
- To synthesize the leading strand
What is the characteristic feature of telomeres?
What is the characteristic feature of telomeres?
What is the function of the PCNA ring during DNA replication?
What is the function of the PCNA ring during DNA replication?
What is the characteristic of the G-rich strand at the telomere end?
What is the characteristic of the G-rich strand at the telomere end?
What is the mode of DNA replication?
What is the mode of DNA replication?
Which of the following is NOT a function of helicase in DNA replication?
Which of the following is NOT a function of helicase in DNA replication?
What is the function of RPA in DNA replication?
What is the function of RPA in DNA replication?
What is the main function of topoisomerase in DNA replication?
What is the main function of topoisomerase in DNA replication?
Which DNA polymerase is involved in leading strand synthesis?
Which DNA polymerase is involved in leading strand synthesis?
What is the function of PCNA in DNA replication?
What is the function of PCNA in DNA replication?
What is the primary function of primase in DNA replication?
What is the primary function of primase in DNA replication?
What is the characteristic of lagging strand synthesis?
What is the characteristic of lagging strand synthesis?
What is the primary reason for the formation of loops at the telomere?
What is the primary reason for the formation of loops at the telomere?
What is the main function of telomeres?
What is the main function of telomeres?
What happens to telomeres during DNA replication?
What happens to telomeres during DNA replication?
What is the consequence of short telomeres?
What is the consequence of short telomeres?
What is the role of telomere-specific proteins?
What is the role of telomere-specific proteins?
What is the effect of short telomeres on the CNS?
What is the effect of short telomeres on the CNS?
What is the relationship between telomere shortening and disease?
What is the relationship between telomere shortening and disease?
What is the function of the ORC and MCM proteins in DNA replication?
What is the function of the ORC and MCM proteins in DNA replication?
In what direction does DNA synthesis always proceed?
In what direction does DNA synthesis always proceed?
What is the function of DNA primers in DNA replication?
What is the function of DNA primers in DNA replication?
What is the result of DNA replication?
What is the result of DNA replication?
What is the function of helicase in DNA replication?
What is the function of helicase in DNA replication?
What is the function of topoisomerase in DNA replication?
What is the function of topoisomerase in DNA replication?
How does DNA polymerase initiate chain synthesis?
How does DNA polymerase initiate chain synthesis?
What is the characteristic of DNA replication?
What is the characteristic of DNA replication?
What is the direction of DNA replication?
What is the direction of DNA replication?
ThePrimase complex synthesizes short RNA _______ of approximately 10nt.
ThePrimase complex synthesizes short RNA _______ of approximately 10nt.
The _______ strand is synthesized by a complex of DNA polymerase δ (Pol δ)-PCNA-Rfc.
The _______ strand is synthesized by a complex of DNA polymerase δ (Pol δ)-PCNA-Rfc.
During concurrent DNA replication, both the _______ and leading strands are synthesized simultaneously.
During concurrent DNA replication, both the _______ and leading strands are synthesized simultaneously.
Ribonucleases are required to remove the RNA _______ from the newly synthesized strand.
Ribonucleases are required to remove the RNA _______ from the newly synthesized strand.
A DNA polymerase adds deoxynucleotides to the free hydroxyl group at the ______ end of the primer
A DNA polymerase adds deoxynucleotides to the free hydroxyl group at the ______ end of the primer
Primases are required to form the ______ primers required for DNA polymerases
Primases are required to form the ______ primers required for DNA polymerases
The G-rich strand at the telomere end terminates in a single-stranded _______ of approximately 75-300 nucleotides.
The G-rich strand at the telomere end terminates in a single-stranded _______ of approximately 75-300 nucleotides.
DNA replication is described as ______-discontinuous due to the different mechanisms of leading and lagging strand synthesis
DNA replication is described as ______-discontinuous due to the different mechanisms of leading and lagging strand synthesis
Topoisomerases are required to ______ the tensions created by the replication bubble
Topoisomerases are required to ______ the tensions created by the replication bubble
Helicases are required for the ______ of the parental DNA strands
Helicases are required for the ______ of the parental DNA strands
Most eukaryotic chromosomes bend back on themselves to form a physical ______ at the telomere.
Most eukaryotic chromosomes bend back on themselves to form a physical ______ at the telomere.
The main function of telomeres is ______.
The main function of telomeres is ______.
Telomeres are recognized by a series of proteins that can be classified as ______ proteins and telomere non-specific proteins.
Telomeres are recognized by a series of proteins that can be classified as ______ proteins and telomere non-specific proteins.
Short telomeres play a central role in the development of ______ diseases.
Short telomeres play a central role in the development of ______ diseases.
Telomere shortening leads to ______.
Telomere shortening leads to ______.
DNA replication is _______________, meaning that each new DNA has one ‘old’ strand and one ‘new’ strand.
DNA replication is _______________, meaning that each new DNA has one ‘old’ strand and one ‘new’ strand.
The pre-_____________________ complex is formed by ORC proteins and MCM proteins that recognize origins of replication.
The pre-_____________________ complex is formed by ORC proteins and MCM proteins that recognize origins of replication.
DNA polymerase requires a short, preexisting RNA or DNA strand, called a _______________, to begin chain growth.
DNA polymerase requires a short, preexisting RNA or DNA strand, called a _______________, to begin chain growth.
DNA replication is _______________, meaning that it starts at the origin of replication and proceeds in both directions.
DNA replication is _______________, meaning that it starts at the origin of replication and proceeds in both directions.
The enzymatic activity of DNA polymerase is essential for DNA replication, but it cannot initiate chain synthesis de novo and requires a _______________.
The enzymatic activity of DNA polymerase is essential for DNA replication, but it cannot initiate chain synthesis de novo and requires a _______________.
