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Questions and Answers
Which enzyme is responsible for the release of DnaB at the forks during DNA replication?
Which enzyme is responsible for the release of DnaB at the forks during DNA replication?
- Primase DnaG
- DNA polymerase I
- DNA gyrase
- DnaC (correct)
What is the role of DNA polymerase I in E. coli DNA replication?
What is the role of DNA polymerase I in E. coli DNA replication?
- Unwinding the DNA double helix
- Creating Okazaki fragments
- Removing RNA primers and replacing them with DNA (correct)
- Initiating DNA synthesis
What is the function of DNA gyrase during DNA replication?
What is the function of DNA gyrase during DNA replication?
- Initiating DNA synthesis
- Creating Okazaki fragments
- Relaxing supercoiled DNA (correct)
- Releasing DNA polymerase III
What is the function of DnaA during DNA replication?
What is the function of DnaA during DNA replication?
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What is the significance of the Meselson and Stahl experiment in understanding DNA replication?
What is the significance of the Meselson and Stahl experiment in understanding DNA replication?
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Study Notes
DNA Replication Enzymes
- Helicase is responsible for the release of DnaB at the replication forks during DNA replication.
DNA Polymerase I
- In E. coli DNA replication, DNA polymerase I is responsible for repairing RNA primers and filling in gaps left by RNAse H.
DNA Gyrase
- DNA gyrase is an enzyme that relaxes positive supercoils in DNA, allowing replication to proceed.
DnaA Protein
- DnaA is a protein that binds to the origin of replication, unwinding the DNA and allowing helicase to bind, initiating DNA replication.
Meselson and Stahl Experiment
- The Meselson and Stahl experiment demonstrated semi-conservative DNA replication, showing that each new DNA molecule contains one old strand (template) and one new strand synthesized during replication.
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