Podcast
Questions and Answers
What is the role of the 3' OH of the growing strand in DNA replication?
What is the role of the 3' OH of the growing strand in DNA replication?
The 3' OH of the growing strand attacks the high-energy phosphate bond of the incoming nucleotide to initiate the synthesis reaction.
During DNA replication, how are the two strands of the parental duplex separated?
During DNA replication, how are the two strands of the parental duplex separated?
The two strands of the parental duplex separate during DNA replication.
What are the base-pairing rules for DNA replication?
What are the base-pairing rules for DNA replication?
The base-pairing rules for DNA replication are A with T and G with C.
What is meant by 'semiconservative' replication and how was it proven?
What is meant by 'semiconservative' replication and how was it proven?
Signup and view all the answers
What is the asymmetry of the DNA replication fork?
What is the asymmetry of the DNA replication fork?
Signup and view all the answers
During DNA replication, the two strands of the parental duplex separate. What type of replication is this?
During DNA replication, the two strands of the parental duplex separate. What type of replication is this?
Signup and view all the answers
What happens when the 3' OH of the growing strand attacks the high-energy phosphate bond of the incoming nucleotide?
What happens when the 3' OH of the growing strand attacks the high-energy phosphate bond of the incoming nucleotide?
Signup and view all the answers
What is the result of two rounds of DNA replication in a labeled medium?
What is the result of two rounds of DNA replication in a labeled medium?
Signup and view all the answers
What type of bonds are formed between the incoming nucleotides and the template during DNA replication?
What type of bonds are formed between the incoming nucleotides and the template during DNA replication?
Signup and view all the answers
What are the base-pairing rules for DNA replication?
What are the base-pairing rules for DNA replication?
Signup and view all the answers
Study Notes
DNA Replication
- During DNA replication, the 3' OH of the growing strand acts as a nucleophile, attacking the high-energy phosphate bond of the incoming nucleotide, resulting in the formation of a phosphodiester bond.
Separation of Parental Duplex
- The two strands of the parental duplex are separated during DNA replication, a process that occurs continuously.
Base-Pairing Rules
- The base-pairing rules for DNA replication are: Adenine (A) pairs with Thymine (T) and Guanine (G) pairs with Cytosine (C).
Semiconservative Replication
- Semiconservative replication means that each new DNA molecule is composed of one old strand (from the parental duplex) and one new strand synthesized during replication.
- This process was proven through the Meselson-Stahl experiment, which demonstrated the gradual shift from heavy to light DNA density.
Asymmetry of the DNA Replication Fork
- The replication fork is asymmetric, with the leading strand being continuously synthesized in the 5' to 3' direction, while the lagging strand is synthesized in short, discontinuous segments called Okazaki fragments.
Type of Replication
- The separation of the two strands of the parental duplex during DNA replication is an example of semiconservative replication.
Bond Formation
- During DNA replication, phosphodiester bonds are formed between the incoming nucleotides and the template.
Result of Two Rounds of DNA Replication
- After two rounds of DNA replication in a labeled medium, the resulting DNA molecules will have one DNA molecule with all light density and one DNA molecule with half heavy and half light density.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge on DNA replication with this quiz! Learn about the process of DNA replication, including how incoming nucleotides are accepted and the role of the 3' OH in initiating the synthesis reaction.
