DNA Polymerase and DNA Synthesis

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Questions and Answers

In which direction does DNA polymerase synthesize DNA?

  • 5'-2' direction
  • 3'-2' direction
  • 5'-3' direction (correct)
  • 3'-5' direction

What is the role of complementary base pairing in DNA synthesis?

  • It helps in the regulation of gene expression.
  • It prevents the loss of genetic material.
  • It stabilizes the RNA structure during transcription.
  • It determines the sequence of nucleotides added. (correct)

Which component serves as substrates for DNA polymerase during DNA synthesis?

  • Amino acids
  • Deoxyribonucleotides (dNTPs) (correct)
  • Nucleic acids
  • Ribonucleotides (rNTPs)

Which enzyme catalyzes the addition of nucleotides to a growing DNA strand?

<p>DNA polymerase (A)</p> Signup and view all the answers

What is added to the growing DNA strand during replication?

<p>Nucleotides to the 3' end (B)</p> Signup and view all the answers

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Study Notes

DNA Polymerase and DNA Synthesis

  • DNA polymerase (DNA Pol) is an enzyme responsible for adding nucleotides to the 3' end of a growing DNA strand.
  • Nucleotides are added in a 5' to 3' direction, meaning the new nucleotide is added to the free 3'-hydroxyl group of the previous nucleotide.
  • The substrates used for DNA synthesis are deoxyribonucleoside triphosphates (dNTPs), which are composed of a deoxyribose sugar, a phosphate group, and a nitrogenous base.
  • DNA Pol adds nucleotides based on complementary base pairing with the template strand, ensuring accurate replication of the genetic code.
  • This process involves hydrogen bonding between complementary base pairs (A with T, and G with C).

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