DNA Fingerprinting Part II: Gel Electrophoresis

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What is the primary factor that affects the migration of charged molecules during electrophoresis?

The size of the molecules (correct)

The pH of the solution

The charge of the molecules

The temperature of the experiment

Which type of gel is typically used to determine the nucleotide sequence of DNA?

Agarose gel

Silica gel

Polyacrylamide gel (correct)

Sephadex gel

Which of the following is true about the relationship between gel concentration and pore size in gel electrophoresis?

Lower gel concentration results in smaller pores (correct)

Gel concentration has no effect on pore size

Pore size is determined by the type of gel, not the concentration

Higher gel concentration results in larger pores

What is the purpose of using different gel concentrations in gel electrophoresis?

To separate larger and smaller DNA fragments Signup and view all the answers

Why are lower voltages and longer run times preferred for the best resolution in gel electrophoresis?

To allow the molecules to migrate more slowly and separate better Signup and view all the answers

Which of the following is a key advantage of using porous gels in electrophoresis?

Enhanced separation of biological molecules Signup and view all the answers

What is the main purpose of electrophoresis in DNA fingerprinting analysis?

To separate molecules based on their size Signup and view all the answers

How do DNA and RNA migrate in an electric field during electrophoresis?

Toward the positive pole Signup and view all the answers

What determines the direction in which proteins migrate during electrophoresis in general?

Both positive and negative charges Signup and view all the answers

Why are proteins sometimes coated with substances that give them a uniform charge of a single type for electrophoresis?

To compel migration in a specific direction Signup and view all the answers

How are molecules typically separated during electrophoresis?

Based on their molecular weight Signup and view all the answers

What differentiates the migration of DNA/RNA and proteins in an electric field during electrophoresis?

Net charge Signup and view all the answers

DNA Fingerprinting Part II: Gel Electrophoresis

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Questions and Answers

What is the primary factor that affects the migration of charged molecules during electrophoresis?

Which type of gel is typically used to determine the nucleotide sequence of DNA?

Which of the following is true about the relationship between gel concentration and pore size in gel electrophoresis?

What is the purpose of using different gel concentrations in gel electrophoresis?

Why are lower voltages and longer run times preferred for the best resolution in gel electrophoresis?

Which of the following is a key advantage of using porous gels in electrophoresis?

What is the main purpose of electrophoresis in DNA fingerprinting analysis?

How do DNA and RNA migrate in an electric field during electrophoresis?

What determines the direction in which proteins migrate during electrophoresis in general?

Why are proteins sometimes coated with substances that give them a uniform charge of a single type for electrophoresis?

How are molecules typically separated during electrophoresis?

What differentiates the migration of DNA/RNA and proteins in an electric field during electrophoresis?

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