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Questions and Answers
What is the primary factor that affects the migration of charged molecules during electrophoresis?
What is the primary factor that affects the migration of charged molecules during electrophoresis?
- The size of the molecules (correct)
- The pH of the solution
- The charge of the molecules
- The temperature of the experiment
Which type of gel is typically used to determine the nucleotide sequence of DNA?
Which type of gel is typically used to determine the nucleotide sequence of DNA?
- Agarose gel
- Silica gel
- Polyacrylamide gel (correct)
- Sephadex gel
Which of the following is true about the relationship between gel concentration and pore size in gel electrophoresis?
Which of the following is true about the relationship between gel concentration and pore size in gel electrophoresis?
- Lower gel concentration results in smaller pores (correct)
- Gel concentration has no effect on pore size
- Pore size is determined by the type of gel, not the concentration
- Higher gel concentration results in larger pores
What is the purpose of using different gel concentrations in gel electrophoresis?
What is the purpose of using different gel concentrations in gel electrophoresis?
Why are lower voltages and longer run times preferred for the best resolution in gel electrophoresis?
Why are lower voltages and longer run times preferred for the best resolution in gel electrophoresis?
Which of the following is a key advantage of using porous gels in electrophoresis?
Which of the following is a key advantage of using porous gels in electrophoresis?
What is the main purpose of electrophoresis in DNA fingerprinting analysis?
What is the main purpose of electrophoresis in DNA fingerprinting analysis?
How do DNA and RNA migrate in an electric field during electrophoresis?
How do DNA and RNA migrate in an electric field during electrophoresis?
What determines the direction in which proteins migrate during electrophoresis in general?
What determines the direction in which proteins migrate during electrophoresis in general?
Why are proteins sometimes coated with substances that give them a uniform charge of a single type for electrophoresis?
Why are proteins sometimes coated with substances that give them a uniform charge of a single type for electrophoresis?
How are molecules typically separated during electrophoresis?
How are molecules typically separated during electrophoresis?
What differentiates the migration of DNA/RNA and proteins in an electric field during electrophoresis?
What differentiates the migration of DNA/RNA and proteins in an electric field during electrophoresis?
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