DNA Fingerprinting Part II: Gel Electrophoresis
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Questions and Answers

What is the primary factor that affects the migration of charged molecules during electrophoresis?

  • The size of the molecules (correct)
  • The pH of the solution
  • The charge of the molecules
  • The temperature of the experiment

    • Which type of gel is typically used to determine the nucleotide sequence of DNA?

  • Agarose gel
  • Silica gel
  • Polyacrylamide gel (correct)
  • Sephadex gel

    • Which of the following is true about the relationship between gel concentration and pore size in gel electrophoresis?

  • Lower gel concentration results in smaller pores (correct)
  • Gel concentration has no effect on pore size
  • Pore size is determined by the type of gel, not the concentration
  • Higher gel concentration results in larger pores

    • What is the purpose of using different gel concentrations in gel electrophoresis?

    <p>To separate larger and smaller DNA fragments</p> Signup and view all the answers

    Why are lower voltages and longer run times preferred for the best resolution in gel electrophoresis?

    <p>To allow the molecules to migrate more slowly and separate better</p> Signup and view all the answers

    Which of the following is a key advantage of using porous gels in electrophoresis?

    <p>Enhanced separation of biological molecules</p> Signup and view all the answers

    What is the main purpose of electrophoresis in DNA fingerprinting analysis?

    <p>To separate molecules based on their size</p> Signup and view all the answers

    How do DNA and RNA migrate in an electric field during electrophoresis?

    <p>Toward the positive pole</p> Signup and view all the answers

    What determines the direction in which proteins migrate during electrophoresis in general?

    <p>Both positive and negative charges</p> Signup and view all the answers

    Why are proteins sometimes coated with substances that give them a uniform charge of a single type for electrophoresis?

    <p>To compel migration in a specific direction</p> Signup and view all the answers

    How are molecules typically separated during electrophoresis?

    <p>Based on their molecular weight</p> Signup and view all the answers

    What differentiates the migration of DNA/RNA and proteins in an electric field during electrophoresis?

    <p>Net charge</p> Signup and view all the answers

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