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DNA Extraction Essentials Quiz with Dr

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Questions and Answers

What is the definition of DNA extraction?

The destruction of DNA within cells or viruses
The modification of DNA within cells or viruses
The removal of DNA from cells or viruses where it normally resides (correct)
The amplification of DNA in cells or viruses

What is a common use of DNA extraction?

Producing synthetic DNA strands
An early step in diagnosing diseases and genetic disorders (correct)
Studying the structure of RNA molecules
Creating genetically modified organisms

How is DNA precipitated during the extraction process?

Mixing with cold ethanol or isopropanol and then centrifuging (correct)
Shaking vigorously for an extended period
Exposing to ultraviolet light
Adding water and heating the solution

What aids in the precipitation of proteins during DNA extraction?

Addition of a salt such as ammonium or sodium acetate (A) Signup and view all the answers

What happens to DNA when mixed with alcohol during extraction?

It becomes insoluble and precipitates out (A) Signup and view all the answers

What is the purpose of sonicating the sample in DNA extraction?

To break open the cells or virus containing DNA (C) Signup and view all the answers

What is the role of protease in DNA extraction?

To degrade DNA-associated proteins (C) Signup and view all the answers

How is the precipitation of protein aided during DNA extraction?

By addition of a salt such as ammonium or sodium acetate (C) Signup and view all the answers

What is the purpose of mixing DNA with cold ethanol or isopropanol during extraction?

To precipitate DNA (C) Signup and view all the answers

What happens to DNA when centrifuged after mixing with cold ethanol or isopropanol?

It becomes insoluble and precipitates (A) Signup and view all the answers