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Questions and Answers
What is the first step in DNA extraction?
What is the first step in DNA extraction?
- Separation of the soluble DNA from cell debris
- Elution of the DNA
- Disruption of the cellular structure to create a lysate (correct)
- Binding the DNA of interest to a purification matrix
How is clearing of lysate usually accomplished?
How is clearing of lysate usually accomplished?
- Washing proteins and other contaminants away from the matrix
- Physical methods, enzymatic methods, chemical methods
- Binding the DNA of interest to a purification matrix
- Centrifugation, filtration or bead-based methods (correct)
What is the purpose of clearing cellular lysates?
What is the purpose of clearing cellular lysates?
- To bind the DNA of interest to a purification matrix
- To reduce the carryover of unwanted materials into the purification reaction (correct)
- To elute the DNA
- To disrupt cells in a sample
What are the general techniques for lysing materials?
What are the general techniques for lysing materials?
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1.5
1.5
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