DNA Purification Basics Quiz

Separation of the soluble DNA from cell debris

Elution of the DNA

Disruption of the cellular structure to create a lysate (correct)

Binding the DNA of interest to a purification matrix

Washing proteins and other contaminants away from the matrix

Physical methods, enzymatic methods, chemical methods

Binding the DNA of interest to a purification matrix

Centrifugation, filtration or bead-based methods (correct)

To bind the DNA of interest to a purification matrix

To reduce the carryover of unwanted materials into the purification reaction (correct)

To elute the DNA

To disrupt cells in a sample

<p>Physical methods, enzymatic methods, chemical methods and combinations of the three</p> Signup and view all the answers

<p>Which method requires more hands-on time for clearing cellular debris?</p> Signup and view all the answers