Podcast
Questions and Answers
What is the purpose of the running buffer in DNA electrophoresis?
What is the purpose of the running buffer in DNA electrophoresis?
- To give DNA the color so it can be seen when loaded in the gel
- To maintain a constant pH and provide ions for conductivity (correct)
- To visualize separated DNA molecules
- To increase the density of the DNA samples
Which buffers are commonly used in gel preparation for DNA electrophoresis?
Which buffers are commonly used in gel preparation for DNA electrophoresis?
- Acetic acid and citric acid
- Tris Borate EDTA (TBE) and Tris Acetate EDTA (TAE) (correct)
- Ammonium hydroxide and hydrochloric acid
- Sodium chloride and potassium sulfate
What is the purpose of adding a loading buffer to DNA samples before loading them in the gel?
What is the purpose of adding a loading buffer to DNA samples before loading them in the gel?
- To increase the density of the DNA samples so they sink in the wells (correct)
- To maintain a constant pH and provide ions for conductivity
- To visualize separated DNA molecules
- To give DNA the color so it can be seen when loaded in the gel
What is the purpose of the colored dye in the loading buffer for DNA samples?
What is the purpose of the colored dye in the loading buffer for DNA samples?
Why is a density agent added to the loading buffer for DNA samples?
Why is a density agent added to the loading buffer for DNA samples?
What is the role of the power source in DNA electrophoresis?
What is the role of the power source in DNA electrophoresis?
Why does DNA move towards the positive end during electrophoresis?
Why does DNA move towards the positive end during electrophoresis?
What is the main focus of Healthcare Epidemiology?
What is the main focus of Healthcare Epidemiology?
What are the common types of nosocomial infections?
What are the common types of nosocomial infections?
What does Healthcare-Associated Infections (HAIs) include?
What does Healthcare-Associated Infections (HAIs) include?
What are the aims of Healthcare Epidemiology?
What are the aims of Healthcare Epidemiology?
What do Infection prevention and control (IPC) measures cover?
What do Infection prevention and control (IPC) measures cover?
What are Community-Associated Infections?
What are Community-Associated Infections?
What are Iatrogenic Infections?
What are Iatrogenic Infections?
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What does Healthcare-Associated Infections (HAIs) include?
What does Healthcare-Associated Infections (HAIs) include?
What are the major factors contributing to nosocomial infections?
What are the major factors contributing to nosocomial infections?
Which pathogen is differentiated from Staphylococcus aureus by being more resistant to antibiotics and found on our skin more?
Which pathogen is differentiated from Staphylococcus aureus by being more resistant to antibiotics and found on our skin more?
Which pathogen is a spore-forming, anaerobic, Gram-positive bacillus and an important cause of antibiotic-associated diarrhea and pseudomembranous colitis?
Which pathogen is a spore-forming, anaerobic, Gram-positive bacillus and an important cause of antibiotic-associated diarrhea and pseudomembranous colitis?
Which Gram-negative bacillus is commonly found in wet environments and is resistant to antibiotics and sterilization techniques?
Which Gram-negative bacillus is commonly found in wet environments and is resistant to antibiotics and sterilization techniques?
Which bacteria are included in the multidrug-resistant Gram-negative bacilli (MDR-GNB)?
Which bacteria are included in the multidrug-resistant Gram-negative bacilli (MDR-GNB)?
Which pathogen is part of the normal flora in the colon and can cause intra-abdominal, liver, and urinary tract infections?
Which pathogen is part of the normal flora in the colon and can cause intra-abdominal, liver, and urinary tract infections?
Which pathogen is found in the environment, causes RT and bloodstream infections, and resists antibiotics and sterilization techniques?
Which pathogen is found in the environment, causes RT and bloodstream infections, and resists antibiotics and sterilization techniques?
What is the main focus of Healthcare Epidemiology?
What is the main focus of Healthcare Epidemiology?
What do Infection prevention and control (IPC) measures cover?
What do Infection prevention and control (IPC) measures cover?
What are the aims of Healthcare Epidemiology?
What are the aims of Healthcare Epidemiology?
What does Healthcare-Associated Infections (HAIs) include?
What does Healthcare-Associated Infections (HAIs) include?
What are Community-Associated Infections?
What are Community-Associated Infections?
What are Iatrogenic Infections?
What are Iatrogenic Infections?
What is the most common type of nosocomial infection?
What is the most common type of nosocomial infection?
What is a characteristic of Healthcare-onset infections?
What is a characteristic of Healthcare-onset infections?
What is the main focus of Surveillance measures in Healthcare Epidemiology?
What is the main focus of Surveillance measures in Healthcare Epidemiology?
Which type of infection is considered a Community-Onset Infection?
Which type of infection is considered a Community-Onset Infection?
What is a major factor contributing to nosocomial infections?
What is a major factor contributing to nosocomial infections?
Which of the following pathogens is part of the colonic normal flora and is an important cause of antibiotic-associated diarrhea?
Which of the following pathogens is part of the colonic normal flora and is an important cause of antibiotic-associated diarrhea?
Which Gram-negative bacillus is commonly found in wet environments and is resistant to antibiotics and sterilization techniques?
Which Gram-negative bacillus is commonly found in wet environments and is resistant to antibiotics and sterilization techniques?
What differentiates Coagulase-ve staph. (S. epidermidis) from Staphylococcus aureus?
What differentiates Coagulase-ve staph. (S. epidermidis) from Staphylococcus aureus?
Which bacteria are included in the multidrug-resistant Gram-negative bacilli (MDR-GNB)?
Which bacteria are included in the multidrug-resistant Gram-negative bacilli (MDR-GNB)?
What kind of infections can be caused by Enterobacteriaceae, part of the normal flora in the colon?
What kind of infections can be caused by Enterobacteriaceae, part of the normal flora in the colon?
What pathogen is differentiated from Staphylococcus aureus by being more resistant to antibiotics and found on our skin more?
What pathogen is differentiated from Staphylococcus aureus by being more resistant to antibiotics and found on our skin more?
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