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Questions and Answers
What volume of disinfectant should be placed into each sterile tube before adding bacteria?
What volume of disinfectant should be placed into each sterile tube before adding bacteria?
At what temperature should the tubes be incubated for 48 hours?
At what temperature should the tubes be incubated for 48 hours?
How much of the bacteria mixture is transferred to the tryptic soy broth at each interval?
How much of the bacteria mixture is transferred to the tryptic soy broth at each interval?
Which microorganisms are specifically mentioned for use in the procedure?
Which microorganisms are specifically mentioned for use in the procedure?
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What should be recorded after shaking and observing the tubes for growth?
What should be recorded after shaking and observing the tubes for growth?
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Study Notes
Disinfection Procedure (Procedure No. 30)
- Select a disinfectant and dilute as per product instructions.
- Add 5 mL of disinfectant to two sterile tubes.
- Introduce 0.05 mL of P. aeruginosa to one tube and 0.05 mL of S. aureus to the other. (Other microbes are acceptable).
- Label tubes appropriately.
- Mix each tube to create a homogeneous suspension.
- At 1, 2, 5, 10, and 15 minutes, transfer 0.1 mL of each mixture to separate tubes of tryptic soy broth. This applies to both bacteria.
- Inoculate two broth tubes with 0.1 mL of each bacteria strain, labeling these as "controls."
- Incubate all tubes at 35°C for 48 hours.
- Shake and monitor each tube for bacterial growth.
- Record growth (+) or no growth (-) for each tube.
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Description
Test your knowledge on the disinfection procedure involving P. aeruginosa and S. aureus. This quiz covers the steps required to properly execute the procedure, including dilution, mixing, and monitoring bacterial growth. Review your understanding of how to identify bacterial growth in this practical lab context.
