Podcast
Questions and Answers
What is the initial step in the disinfection procedure?
What is the initial step in the disinfection procedure?
- Select a disinfectant and dilute it if necessary. (correct)
- Label the sterile tubes accordingly.
- Mix the disinfectant with sterile water.
- Add 0.05 mL of P. aeruginosa to the disinfectant.
At which time intervals should samples be transferred to tryptic soy broth?
At which time intervals should samples be transferred to tryptic soy broth?
- 0, 5, 10, and 20 minutes
- 1, 5, 10, and 30 minutes
- 1, 3, 5, 10, and 15 minutes
- 1, 2, 5, 10, and 15 minutes (correct)
How much of each disinfectant solution is placed into the sterile tubes?
How much of each disinfectant solution is placed into the sterile tubes?
- 10 mL of disinfectant
- 5 mL of disinfectant (correct)
- 0.1 mL of disinfectant
- 0.05 mL of disinfectant
What should be done after adding bacteria to each tube?
What should be done after adding bacteria to each tube?
Which microorganisms can be used in this disinfection procedure?
Which microorganisms can be used in this disinfection procedure?
Flashcards
Disinfectant Dilution
Disinfectant Dilution
Selecting the right disinfectant for the job and making sure it's diluted properly, following the manufacturer's guidelines
P. aeruginosa
P. aeruginosa
A specific type of bacteria known for its resistance and difficult-to-kill nature. It is commonly used in testing the effectiveness of disinfectants, as it represents a tough challenge
S. Aureus
S. Aureus
A specific type of bacteria commonly used in testing the effectiveness of disinfectants due to its relative ease of growth and frequent presence in skin infections
Tryptic Soy Broth Transfer
Tryptic Soy Broth Transfer
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Homogenous Suspension
Homogenous Suspension
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Study Notes
Disinfection Procedure
- Disinfectant selection and dilution: Choose a disinfectant and dilute it as per manufacturer's instructions if needed.
- Sample preparation: Transfer 5 mL of disinfectant to two sterile tubes. Add 0.05 mL of P. aeruginosa to one tube and 0.05 mL of S. aureus to the other tube. (Other microorganisms are possible).
- Labeling: Label the tubes clearly indicating the contents.
- Mixing: Mix each tube thoroughly to create a uniform suspension of bacteria and disinfectant.
- Timed samples: At 1, 2, 5, 10, and 15 minutes, take 0.1 mL of the mixture from each tube and transfer it to separate tubes of tryptic soy broth. This is done for both P. aeruginosa and S. aureus.
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Description
Test your knowledge of disinfection procedures involving P. aeruginosa and S. aureus. This quiz covers key steps such as disinfectant selection, sample preparation, mixing, and timed sampling. See how well you understand the disinfection process and its microbiological implications.
