Disinfection Procedure Quiz

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Questions and Answers

What is the initial step in the disinfection procedure?

Select a disinfectant and dilute it if necessary. (correct)
Label the sterile tubes accordingly.
Mix the disinfectant with sterile water.
Add 0.05 mL of P. aeruginosa to the disinfectant.

At which time intervals should samples be transferred to tryptic soy broth?

0, 5, 10, and 20 minutes
1, 5, 10, and 30 minutes
1, 3, 5, 10, and 15 minutes
1, 2, 5, 10, and 15 minutes (correct)

How much of each disinfectant solution is placed into the sterile tubes?

10 mL of disinfectant
5 mL of disinfectant (correct)
0.1 mL of disinfectant
0.05 mL of disinfectant

What should be done after adding bacteria to each tube?

<p>Mix each tube to ensure a homogenous suspension. (A)</p> Signup and view all the answers

Which microorganisms can be used in this disinfection procedure?

<p>Any microorganisms, with examples provided (A)</p> Signup and view all the answers

Flashcards

Disinfectant Dilution

Selecting the right disinfectant for the job and making sure it's diluted properly, following the manufacturer's guidelines

P. aeruginosa

A specific type of bacteria known for its resistance and difficult-to-kill nature. It is commonly used in testing the effectiveness of disinfectants, as it represents a tough challenge

S. Aureus

A specific type of bacteria commonly used in testing the effectiveness of disinfectants due to its relative ease of growth and frequent presence in skin infections

Tryptic Soy Broth Transfer

A technique where a small amount of a liquid mixture (containing both bacteria and disinfectant) is transferred to a nutrient-rich broth, allowing any remaining bacteria to grow and become visible. This method helps determine if the disinfectant has effectively killed all the bacteria.

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Homogenous Suspension

The process of mixing the bacteria and disinfectant thoroughly, making sure they have even contact for accurate testing.

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Study Notes

Disinfection Procedure

Disinfectant selection and dilution: Choose a disinfectant and dilute it as per manufacturer's instructions if needed.
Sample preparation: Transfer 5 mL of disinfectant to two sterile tubes. Add 0.05 mL of P. aeruginosa to one tube and 0.05 mL of S. aureus to the other tube. (Other microorganisms are possible).
Labeling: Label the tubes clearly indicating the contents.
Mixing: Mix each tube thoroughly to create a uniform suspension of bacteria and disinfectant.
Timed samples: At 1, 2, 5, 10, and 15 minutes, take 0.1 mL of the mixture from each tube and transfer it to separate tubes of tryptic soy broth. This is done for both P. aeruginosa and S. aureus.