Podcast
Questions and Answers
What is the initial step in the disinfection procedure?
What is the initial step in the disinfection procedure?
At which time intervals should samples be transferred to tryptic soy broth?
At which time intervals should samples be transferred to tryptic soy broth?
How much of each disinfectant solution is placed into the sterile tubes?
How much of each disinfectant solution is placed into the sterile tubes?
What should be done after adding bacteria to each tube?
What should be done after adding bacteria to each tube?
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Which microorganisms can be used in this disinfection procedure?
Which microorganisms can be used in this disinfection procedure?
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Study Notes
Disinfection Procedure
- Disinfectant selection and dilution: Choose a disinfectant and dilute it as per manufacturer's instructions if needed.
- Sample preparation: Transfer 5 mL of disinfectant to two sterile tubes. Add 0.05 mL of P. aeruginosa to one tube and 0.05 mL of S. aureus to the other tube. (Other microorganisms are possible).
- Labeling: Label the tubes clearly indicating the contents.
- Mixing: Mix each tube thoroughly to create a uniform suspension of bacteria and disinfectant.
- Timed samples: At 1, 2, 5, 10, and 15 minutes, take 0.1 mL of the mixture from each tube and transfer it to separate tubes of tryptic soy broth. This is done for both P. aeruginosa and S. aureus.
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Description
Test your knowledge of disinfection procedures involving P. aeruginosa and S. aureus. This quiz covers key steps such as disinfectant selection, sample preparation, mixing, and timed sampling. See how well you understand the disinfection process and its microbiological implications.
