Podcast
Questions and Answers
What technique was used to detect CMV in perilymphatic fluid?
What technique was used to detect CMV in perilymphatic fluid?
- ELISA
- Serology
- QRTPCR (correct)
- Cul-ture
In how many patients was QRTPCR positive for CMV in perilymphatic fluid?
In how many patients was QRTPCR positive for CMV in perilymphatic fluid?
- None of the patients
- Two patients
- Four patients (correct)
- Six patients
What was the result of blood analysis for CMV using QRTPCR?
What was the result of blood analysis for CMV using QRTPCR?
- Not analyzed
- Negative in all patients (correct)
- Positive in all patients
- Positive in some patients
What is the estimated volume of perilymph in the human cochlea?
What is the estimated volume of perilymph in the human cochlea?
What is the positive predictive value of viral culture for detecting CMV?
What is the positive predictive value of viral culture for detecting CMV?
How does CMV theorized to reach the inner ear?
How does CMV theorized to reach the inner ear?
What condition is being investigated in relation to CMV presence in perilymphatic fluid?
What condition is being investigated in relation to CMV presence in perilymphatic fluid?
What factor affects the sensitivity of the viral culture assay for CMV detection?
What factor affects the sensitivity of the viral culture assay for CMV detection?
What is the estimated incidence of congenital cytomegalovirus (CMV) infection in neonates?
What is the estimated incidence of congenital cytomegalovirus (CMV) infection in neonates?
What percentage of congenitally infected infants with CMV are considered asymptomatic at birth?
What percentage of congenitally infected infants with CMV are considered asymptomatic at birth?
What range of percentage does sensorineural hearing loss (SNHL) occur in asymptomatic congenital CMV infections?
What range of percentage does sensorineural hearing loss (SNHL) occur in asymptomatic congenital CMV infections?
What percentage of infants with congenital CMV are expected to have bilateral profound SNHL?
What percentage of infants with congenital CMV are expected to have bilateral profound SNHL?
What method was used to analyze perilymphatic fluid for the presence of CMV?
What method was used to analyze perilymphatic fluid for the presence of CMV?
What percentage of all cases of congenital sensorineural hearing loss (SNHL) is attributed to CMV according to recent literature?
What percentage of all cases of congenital sensorineural hearing loss (SNHL) is attributed to CMV according to recent literature?
What is known about the pathogenesis of CMV-mediated hearing loss?
What is known about the pathogenesis of CMV-mediated hearing loss?
What was the primary study design used to determine the presence of CMV in perilymphatic fluid?
What was the primary study design used to determine the presence of CMV in perilymphatic fluid?
What method was used to collect perilymphatic fluid during the procedure?
What method was used to collect perilymphatic fluid during the procedure?
What was the purpose of centrifuging the perilymphatic fluid specimen?
What was the purpose of centrifuging the perilymphatic fluid specimen?
What was the concentration of CMV in the blood for the studied patients?
What was the concentration of CMV in the blood for the studied patients?
Which patient had a concentration of CMV higher than would be expected in a positive blood sample?
Which patient had a concentration of CMV higher than would be expected in a positive blood sample?
What was the primary technique used to detect the presence of CMV in perilymphatic fluid?
What was the primary technique used to detect the presence of CMV in perilymphatic fluid?
What was the sampling method for blood collection during the study?
What was the sampling method for blood collection during the study?
What was the initial clinical presentation of the children involved in the study?
What was the initial clinical presentation of the children involved in the study?
In what year was the study published?
In what year was the study published?
What is a significant factor in quantifying CMV DNA copy numbers in immune-suppressed individuals?
What is a significant factor in quantifying CMV DNA copy numbers in immune-suppressed individuals?
What complicating factor may affect molecular epidemiological studies related to CMV?
What complicating factor may affect molecular epidemiological studies related to CMV?
Why might patients 1 and 4 have lower CMV concentrations compared to patients 2 and 6?
Why might patients 1 and 4 have lower CMV concentrations compared to patients 2 and 6?
What clinical condition has been linked to congenital cytomegalovirus infection according to the content?
What clinical condition has been linked to congenital cytomegalovirus infection according to the content?
What limitation does QRTPCR have in the study of CMV?
What limitation does QRTPCR have in the study of CMV?
What does the interaction of CMV with endothelial cells depend on?
What does the interaction of CMV with endothelial cells depend on?
What was the outcome of the clinical pathologic study of hearing loss linked to congenital CMV?
What was the outcome of the clinical pathologic study of hearing loss linked to congenital CMV?
What aspect does the case study involving temporal bone histopathology focus on?
What aspect does the case study involving temporal bone histopathology focus on?
What percentage of cases of congenital nonsyndromic sensorineural hearing loss is suggested to be related to CMV infection?
What percentage of cases of congenital nonsyndromic sensorineural hearing loss is suggested to be related to CMV infection?
What is the primary objective of using QRTPCR in this study?
What is the primary objective of using QRTPCR in this study?
How many children were identified during the precochlear implant evaluation process for this study?
How many children were identified during the precochlear implant evaluation process for this study?
What unique feature did one patient have among the identified cases?
What unique feature did one patient have among the identified cases?
What were the main findings on the MRI of two children in the study?
What were the main findings on the MRI of two children in the study?
Which institution approved the project?
Which institution approved the project?
What is the main benefit of using QRTPCR in this research context?
What is the main benefit of using QRTPCR in this research context?
What type of surgery were the children identified undergoing evaluation for?
What type of surgery were the children identified undergoing evaluation for?
Study Notes
Cytomegalovirus (CMV) in the Perilymphatic Fluid
- CMV infection is an important cause of congenital sensorineural hearing loss (SNHL)
- Approximately 1% of newborns are infected with CMV at birth
- 90% of congenitally infected infants are asymptomatic, meaning they show no signs or symptoms at birth
- The prevalence of SNHL in asymptomatic, congenitally infected infants ranges from 7% to 15%
- CMV is found in the perilymphatic fluid of some children with congenital SNHL
- CMV can be detected and quantified in perilymphatic fluid using quantitative real-time polymerase chain reaction (QRTPCR)
- Studying perilymphatic fluid after cochlear implantation can help to understand the cause of congenital SNHL
- The study found that CMV was present in the perilymphatic fluid of four out of six children with congenital CMV infection
- The blood of all six children tested negative for CMV, suggesting that the virus was present in the inner ear and not a contaminant
- The concentration of CMV in perilymphatic fluid varied between patients
- This study suggests that CMV may play a role in the pathogenesis of SNHL in some children with congenital CMV infection
- More research is needed to determine the prevalence of CMV-mediated profound HL in children with unknown etiology
Materials and Methods
- Six children were enrolled in the study: four with known congenital CMV infection and two with radiologic evidence suggestive of congenital viral infection
- Perilymphatic fluid and blood samples were collected during cochlear implantation
- The perilymphatic fluid was centrifuged to remove surgical contaminants
- DNA was extracted from the perilymphatic fluid and blood samples
- QRTPCR was used to detect and quantify CMV in both samples
Results
- QRTPCR was positive for CMV in the perilymphatic fluid of four patients
- All blood samples were negative for CMV using QRTPCR and culture
Conclusions
- CMV can be detected and quantified in perilymphatic fluid using QRTPCR
- The findings suggest that CMV may be present in the inner ear of children with congenital CMV infection
- More research is needed to determine the prevalence of CMV-mediated profound HL in children with unknown etiology
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Description
This quiz explores the role of Cytomegalovirus (CMV) in congenital sensorineural hearing loss (SNHL), particularly its presence in perilymphatic fluid. Learn about the prevalence of CMV infection in newborns, its asymptomatic nature in most cases, and the implications for cochlear implantation studies. Test your knowledge on these vital topics in pediatric audiology.
