COPY: Cytologic Techniques and Exfoliative Cytology
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Questions and Answers

Which of the following stains is specifically used for staining blood?

  • Acridine
  • Rhodamine dye
  • Giemsa stain (correct)
  • Fluorescein

    • What does the wet film method primarily focus on?

  • Assessment of cellular conditions
  • Staining surrounding tissues (correct)
  • Visualizing distant organ spread
  • Identification of blood vessel structures

    • Which mounting type uses glycerine-jelly?

  • Pern or Dry
  • Permanent
  • Temp or Wet (correct)
  • Indefinite

    • In which class of Pap's cytologic report would you find a normal cytology with 0-25% abnormal cells?

    <p>Class I</p> Signup and view all the answers

    What recent procedure utilizes gamma radiation for scanning?

    <p>Radio Active Scan</p> Signup and view all the answers

    Which type of microscopy would be most suitable for identifying certain organisms?

    <p>Dark field microscopy</p> Signup and view all the answers

    What does the term 'RIA' stand for in recent procedures?

    <p>Radio Immuno Assay</p> Signup and view all the answers

    Which type of staining method relies on an antigen-antibody reaction?

    <p>Immunofluorescence</p> Signup and view all the answers

    What is the primary purpose of exfoliative cytology?

    <p>To study cells obtained from different parts of the body</p> Signup and view all the answers

    Which indicator is a sure sign of fertility according to laboratory diagnosis?

    <p>Formation of palm-leaf or ferning crystal</p> Signup and view all the answers

    What does an increase in the maturation index (MI) indicate?

    <p>Increase in cases of cancer</p> Signup and view all the answers

    What does the absence of fern in a laboratory diagnosis suggest?

    <p>Low estrogen and high progesterone</p> Signup and view all the answers

    Which of these is a common method used in cytology procedures?

    <p>Papanicolaou/Pap's smear</p> Signup and view all the answers

    What does a high pycnotic index (PI) imply?

    <p>Increased number of cells with structureless nuclei</p> Signup and view all the answers

    Which sample type is NOT typically associated with exfoliative cytology?

    <p>Blood culture samples</p> Signup and view all the answers

    How is a Pap's smear prepared during the staining procedure?

    <p>Fixed smear washed in water and rehydrated in descending alcohol solutions</p> Signup and view all the answers

    What is the main goal of the fixation process in tissue processing?

    <p>To kill, harden, and preserve tissues</p> Signup and view all the answers

    Which process involves compressing small pieces of tissue on a microscope slide?

    <p>Squash separation</p> Signup and view all the answers

    Which of the following is NOT a purpose of fixation in tissue processing?

    <p>Enhance metabolic activity</p> Signup and view all the answers

    In what temperature range should tissues be kept during the dissection process in freezing techniques?

    <p>-10°C to -20°C</p> Signup and view all the answers

    Which of the following is a characteristic of a good fixative?

    <p>It must be cheap and stable</p> Signup and view all the answers

    What type of fixative is frequently used in saturated aqueous solutions for renal tissue preservation?

    <p>Mercuric Chloride</p> Signup and view all the answers

    Which histological technique requires the use of differential dyes?

    <p>Teasing or dissociation</p> Signup and view all the answers

    What type of action does autolysis refer to in the context of tissue processing?

    <p>Breakdown by proteolytic enzymes</p> Signup and view all the answers

    Which stain is used specifically for identifying fibrin?

    <p>Hematoxylin and Eosin Stain</p> Signup and view all the answers

    What is the main staining method for glycogen detection?

    <p>Best Carmine Method</p> Signup and view all the answers

    Which stain would you select for staining myelin sheath?

    <p>Weigert-Pal Technique</p> Signup and view all the answers

    For the detection of lipids, which technique is not applicable?

    <p>Gram's Iodine Stain</p> Signup and view all the answers

    Which method is specifically used for identifying astrocytes?

    <p>Mallory's PTAH Stain</p> Signup and view all the answers

    Which staining technique is used for the detection of amyloid?

    <p>Congo Red Method</p> Signup and view all the answers

    What method is suitable for staining mucin?

    <p>Periodic Acid Schiff (PAS)</p> Signup and view all the answers

    Which technique is most appropriate for the identification of phospholipids?

    <p>Sudan Black B</p> Signup and view all the answers

    What is the primary function of Acetone in tissue fixation?

    <p>To fix brain tissues for diagnosis of Rabies</p> Signup and view all the answers

    Which fixative should never contain glacial HAC?

    <p>Flemming's solution</p> Signup and view all the answers

    What type of fixative is Newcomer’s Fluid classified as?

    <p>Cytoplasmic Fixative</p> Signup and view all the answers

    Which solution is known for its use in secondary fixation for better staining?

    <p>Formalin with Post-Chroming</p> Signup and view all the answers

    What is the primary purpose of using a mounting medium?

    <p>To prevent the movement of the cover slip</p> Signup and view all the answers

    What is the primary role of Microanatomical Fixatives?

    <p>To allow general microscopic study of tissue structures</p> Signup and view all the answers

    Which mounting medium is specifically used for Methylene Blue stained nerve preparations?

    <p>Apapy's Medium</p> Signup and view all the answers

    Which of the following is NOT a component of Compound Fixatives?

    <p>Single fixative substance</p> Signup and view all the answers

    What is a key characteristic of Kronig cement?

    <p>It is one of the ringing media to seal cover slips.</p> Signup and view all the answers

    Which fixative is most commonly used for preserving myelin?

    <p>Osmium Tetroxide</p> Signup and view all the answers

    What is the consequence of using a fixative with a pH of more than 4.6?

    <p>It may negatively affect the fixation process.</p> Signup and view all the answers

    Which resinous medium can be neutralized and is known to change color upon exposure?

    <p>Canada Balsam</p> Signup and view all the answers

    What is the refractive index of the medium that should be close to that of glass for better viewing?

    <p>1.518</p> Signup and view all the answers

    What is the purpose of ringing in the specimen preparation process?

    <p>To prevent fluid escapage from the mount</p> Signup and view all the answers

    What type of slides are typically labeled with a gum label?

    <p>General slides for permanent storage</p> Signup and view all the answers

    Which fluid is recommended for mounting frozen sections requiring dehydration?

    <p>Brun's Fluid</p> Signup and view all the answers

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