Cell Biology Week 1 Notes
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Questions and Answers

Who developed the CRISPR/Cas9 system, which won a Nobel Prize in chemistry in 2020?

  • Robert Hooke
  • Jennifer Doudna and Emmanuelle Charpentier (correct)
  • Dr. Craig Venter
  • Antonie van Leeuwenhoek
  • What is the main advantage of the CRISPR/Cas9 system?

  • Discovery of cell biology
  • Precision, reliability, affordability, and simplicity (correct)
  • Ability to create synthetic bacterial cells
  • High magnification power
  • In which century did pioneering scientists begin making the first microscopes?

  • 19th century
  • 17th century (correct)
  • 18th century
  • 16th century
  • Who famously observed thin slivers of cork under a microscope and coined the term 'cell'?

    <p>Robert Hooke</p> Signup and view all the answers

    What field is Dr. Craig Venter known for his innovations in?

    <p>Genetics</p> Signup and view all the answers

    Who was the first person to document human red blood cells?

    <p>Leeuwenhoek</p> Signup and view all the answers

    What is the main difference between transmission electron microscopes (TEM) and scanning electron microscopes (SEM)?

    <p>TEM uses electrons that penetrate through the specimen to show organelles, while SEM uses electrons that scatter along the surface of the specimen to give a 3D image.</p> Signup and view all the answers

    What is the defining characteristic of prokaryotic cells?

    <p>Circular chromosomal DNA confirmed in a region called the nucleoid.</p> Signup and view all the answers

    What did Harold Urey and his grad student Stanley Miller demonstrate in their classic experiment on abiogenesis?

    <p>The possibility of spontaneous synthesis of biological materials under early Earth conditions.</p> Signup and view all the answers

    What does the Endosymbiont Theory proposed by Lynn Margulis explain?

    <p>The engulfment and symbiotic relationship between prokaryotic and eukaryotic cells, leading to the formation of mitochondria and chloroplasts.</p> Signup and view all the answers

    What is the unit used to measure biomolecules such as proteins?

    <p>Angstroms (A)</p> Signup and view all the answers

    What is the defining characteristic of prokaryotic cells?

    <p>Circular chromosomal DNA confirmed in the nucleoid region</p> Signup and view all the answers

    What was the big game changer in DNA manipulation in 2012?

    <p>Development of the CRISPR/Cas9 system</p> Signup and view all the answers

    What is the potential application of CRISPR in health treatments?

    <p>Removing or fixing defective genes</p> Signup and view all the answers

    Who is credited with coining the term 'cell' after observing thin slivers of cork under a microscope?

    <p>Robert Hooke</p> Signup and view all the answers

    What did Dr. Craig Venter innovate in the field related to DNA manipulation?

    <p>He created the first self-replicating synthetic bacterial cell</p> Signup and view all the answers

    Who were the pioneers in making the first microscopes?

    <p>Robert Hooke and Antonie van Leeuwenhoek</p> Signup and view all the answers

    How big are Ribosomes?

    <p>25-30nm</p> Signup and view all the answers

    How big are membranes?

    <p>7-8nm</p> Signup and view all the answers

    How big are microtubules(the largest component of the cytoskeleton)?

    <p>25nm</p> Signup and view all the answers

    How big are microfilaments?

    <p>7nm</p> Signup and view all the answers

    Study Notes

    CRISPR/Cas9 System

    • The CRISPR/Cas9 system was developed by scientists who won a Nobel Prize in Chemistry in 2020.
    • The main advantage of the CRISPR/Cas9 system is its ability to edit genes with precision and efficiency.

    Microscopy

    • Pioneering scientists began making the first microscopes in the 16th century.
    • Antonie van Leeuwenhoek was the first person to document human red blood cells.
    • Matthias Jakob Schleiden and Theodor Schwann famously observed thin slivers of cork under a microscope and coined the term 'cell'.

    Electron Microscopes

    • The main difference between Transmission Electron Microscopes (TEM) and Scanning Electron Microscopes (SEM) is that TEM uses a beam of electrons to produce an image of the sample, while SEM uses a beam of electrons to produce a 3D image of the sample's surface.

    Cell Biology

    • The defining characteristic of prokaryotic cells is the lack of a true nucleus.
    • Ribosomes are approximately 20-30 nanometers (nm) in diameter.
    • Membranes are approximately 5-10 nanometers (nm) thick.
    • Microtubules, the largest component of the cytoskeleton, are approximately 25 nanometers (nm) in diameter.
    • Microfilaments are approximately 7 nanometers (nm) in diameter.

    DNA Manipulation and Abiogenesis

    • Dr. Craig Venter is known for his innovations in the field of DNA manipulation.
    • Dr. Craig Venter innovated in the field of DNA synthesis, creating the first self-replicating synthetic cell.
    • Harold Urey and his graduate student Stanley Miller demonstrated the possibility of abiogenesis in their classic experiment, showing that simple organic compounds can be formed from inorganic substances.
    • The Endosymbiont Theory proposed by Lynn Margulis explains the origin of mitochondria and chloroplasts in eukaryotic cells.

    Biomolecules

    • The unit used to measure biomolecules such as proteins is the Dalton (Da).

    CRISPR Applications

    • The potential application of CRISPR in health treatments is to cure genetic diseases by editing genes with precision and efficiency.
    • The big game changer in DNA manipulation in 2012 was the development of the CRISPR/Cas9 system, which allows for precise and efficient editing of genes.

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    Learn about the CRISPR/Cas9 gene editing system developed by Jennifer Doudna and Emmanuelle Charpentier in 2012, which won them the Nobel Prize in chemistry in 2020. Discover how this precise, reliable, and cost-effective method has revolutionized genetic manipulation for potential health treatments and research.

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