Questions and Answers
When carrying a microscope, grab the ______ with one hand and place your other hand under the base.
Arm
What is the part of the microscope that serves as the bottom support?
Base
In the compound microscope with a ___________________, there are two eyepiece lenses but still only one objective lens.
Binocular Head
What is the purpose of the Coarse Focus knob?
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By using a ________________, you will increase the illumination and resolution on higher power objective lenses.
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What is defined as the relationship between the luminance of an object and its background?
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The ________________ is used to vary the light that passes through the stage opening.
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What type of lens is at the top of the microscope that you look into?
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In a stereo (low power) microscope, there are objective pairs, one lens for each eyepiece lens, giving a 3-D effect. On a high power binocular model there is still only one _________________ so no stereo vision.
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When you look through the eyepiece lens, you may see a ___________.
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____________ is the bending of light as it passes from one substance to another.
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What is the ability of a lens system to show fine details of the object observed called?
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One typically uses the coarse focus first to get close, then moves to the _________________ knob for fine tuning.
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What is the diameter of the circle of light that you see when looking into a microscope called?
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What is a means of moving the specimen closer or further away from the objective lens called?
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What is the light source mounted under the stage of a microscope called?
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Total ___________________ of a microscope is determined by multiplying the magnification capability of the eyepiece lens by that of the objective lens.
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A ______________ is a way to move the slide around on your stage.
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Study Notes
Microscope Parts and Functions
- Arm: Connects the microscope tube to the base; crucial for transport.
- Base: Provides support at the bottom of the microscope.
- Binocular Head: Features two eyepiece lenses; designed for high-powered compound microscopes; differs from stereo heads which have separate objective lenses for each eye.
- Coarse Focus: Rough adjustment knob used to move objective lenses closer or further from the specimen.
- Condenser Lens: Focuses light onto the specimen; necessary for higher power objectives to enhance illumination and resolution.
- Iris Diaphragm: Located under the stage; regulates light intensity and supports contrast and resolution adjustments; essential for high power microscopy.
Optical Elements
- Eyepiece Lens: The top lens providing a view; commonly has a magnification of 10x, with options for 5x, 15x, and 20x available.
- Objective Lens: Closest lens to the specimen; varies in design between stereo (for 3-D effect) and high-powered binocular models, generally providing a single optical path.
- Pointer: An indicator seen through the eyepiece that can be adjusted by rotating the eyepiece.
- Refraction: Describes light bending as it transitions between different materials, crucial for image clarity.
- Resolution: Determines the ability to distinguish fine details in an observed item.
Focusing and Viewing
- Fine Focus: Allows precise focusing adjustments after rough focusing with the coarse knob; critical for detailed specimen examination.
- Field of View: The light circle diameter visible through the eyepiece; shrinks as magnification increases; example measurements include 4.5mm at 40x and 0.18mm at 1000x.
- Focus: The adjustment of the specimen's distance from the lens to achieve a clear image, with varying methods for moving the stage or the tube.
Illumination and Magnification
- Illuminator: Light source beneath the stage; types include Tungsten (hot), Fluorescent (cool and bright), and Halogen (very bright but emits heat).
- Magnification: Core function of a microscope; total magnification is calculated by multiplying eyepiece magnification by objective lens magnification.
- Mechanical Stage: Movement mechanism for slide positioning consisting of a slide holder and knobs for directional adjustments.
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Description
Test your knowledge of key terms related to the compound light microscope. This flashcard set covers important parts of the microscope, including the arm, base, and binocular head. Perfect for students needing to review basic microscope terminology.
