Questions and Answers
What is total magnification?
Equal to the power of the ocular lens multiplied by the power of the objective lens used.
What is resolution in the context of microscopy?
Ability to discriminate between two close objects as separate.
What does parfocal mean?
Property of a microscope that allows objectives to be changed without having to refocus.
What is meant by the term 'field' in microscopy?
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What is depth of field?
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What is working distance?
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Study Notes
Total Magnification
- Total magnification is calculated by multiplying the power of the ocular lens by the power of the objective lens.
- For example, using a 10x ocular lens and a 45x objective lens results in a total magnification of 450x.
Resolution (Resolving Power)
- Resolution refers to the microscope's ability to distinguish between two objects that are close together.
- Higher resolution allows for clearer separation of details in the observed specimen.
Parfocal
- Parfocal is an important property of microscopes, enabling the user to switch between objective lenses without needing to refocus.
- This feature ensures that when changing magnifications, the specimen remains in clear focus.
Field
- The field is defined as the visual area observed through the microscope.
- It can vary based on the objective lens being used, with higher magnifications typically resulting in a smaller field of view.
Depth of Field
- Depth of field describes the thickness of the plane that remains sharply in focus within the microscope's view.
- A greater depth of field allows for more of the specimen's layers to be seen clearly at once.
Working Distance
- Working distance is the measurement of space between the objective lens and the specimen being examined.
- It is crucial for preventing damage to slides or specimens during observation, especially with high-power lenses.
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Description
Enhance your understanding of essential microscope terms with these flashcards. Key concepts include total magnification, resolution, parfocal, field, depth of field, and working distance. Perfect for biology students looking to deepen their knowledge of microscopy.
