Column Chromatography Tutorial

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What is the main purpose of using a plug of cotton in column chromatography?

To prevent loss of the stationary phase (correct)

To maintain the temperature of the column

To speed up the separation process

To prevent loss of the mobile phase

What is the difference between wet packing and dry packing in column chromatography?

Wet packing involves making a slurry of the stationary phase with the mobile phase (correct)

Wet packing uses a different type of stationary phase

Wet packing is used for non-polar compounds

Dry packing uses a higher pressure to pack the column

What is the disadvantage of dry packing in column chromatography?

The stationary phase is not stable

The mobile phase is too expensive

Non-uniform packing and air bubbles formation (correct)

The column is too heavy to handle

What is the purpose of adding a layer of silica to the top of the column during wet loading?

To prevent the distribution of the sample to the mobile phase Signup and view all the answers

What is the term given to the process of adding the mixture to the top of the column in column chromatography?

Loading Signup and view all the answers

What is the purpose of using a vertical glass cylindrical tube in column chromatography?

To facilitate the flow of the mobile phase Signup and view all the answers

What is the primary purpose of using a rotary evaporator in the Dry Loading method?

To remove the solvent and obtain dry and free-flowing silica Signup and view all the answers

Why is it important to ensure that there are no air bubbles in the column during sample loading?

To prevent cracking of the silica Signup and view all the answers

What is the term for the mobile phase used to perform a separation?

Eluent Signup and view all the answers

What is the result of gradually changing the composition of the solvents during sample separation?

Gradient elution Signup and view all the answers

What is the term for the part of the effluent that contains the eluted substance?

Eluate Signup and view all the answers

What is the advantage of using smaller particle sizes in chromatography?

Increased theoretical plates Signup and view all the answers

What is the definition of Retention Volume in chromatography?

The volume of mobile phase required to elute the solute components from the column Signup and view all the answers

What is Dead Time in chromatography?

The time taken for the mobile phase to pass through the column without any interaction with the stationary phase Signup and view all the answers

What is the purpose of using Uracil in reversed phase adsorption?

To measure the Dead Time and Volume of an unretained compound Signup and view all the answers

What is the formula to calculate the Corrected Retention Time?

Retention Time - Dead Time Signup and view all the answers

What is the relationship between Retention Volume and Dead Volume?

Retention Volume is equal to the difference between the Retention Volume and Dead Volume Signup and view all the answers