Column Chromatography Tutorial
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Questions and Answers

What is the main purpose of using a plug of cotton in column chromatography?

  • To prevent loss of the stationary phase (correct)
  • To maintain the temperature of the column
  • To speed up the separation process
  • To prevent loss of the mobile phase

    • What is the difference between wet packing and dry packing in column chromatography?

  • Wet packing involves making a slurry of the stationary phase with the mobile phase (correct)
  • Wet packing uses a different type of stationary phase
  • Wet packing is used for non-polar compounds
  • Dry packing uses a higher pressure to pack the column

    • What is the disadvantage of dry packing in column chromatography?

  • The stationary phase is not stable
  • The mobile phase is too expensive
  • Non-uniform packing and air bubbles formation (correct)
  • The column is too heavy to handle

    • What is the purpose of adding a layer of silica to the top of the column during wet loading?

    <p>To prevent the distribution of the sample to the mobile phase (C)</p> Signup and view all the answers

    What is the term given to the process of adding the mixture to the top of the column in column chromatography?

    <p>Loading (C)</p> Signup and view all the answers

    What is the purpose of using a vertical glass cylindrical tube in column chromatography?

    <p>To facilitate the flow of the mobile phase (D)</p> Signup and view all the answers

    What is the primary purpose of using a rotary evaporator in the Dry Loading method?

    <p>To remove the solvent and obtain dry and free-flowing silica (C)</p> Signup and view all the answers

    Why is it important to ensure that there are no air bubbles in the column during sample loading?

    <p>To prevent cracking of the silica (D)</p> Signup and view all the answers

    What is the term for the mobile phase used to perform a separation?

    <p>Eluent (A)</p> Signup and view all the answers

    What is the result of gradually changing the composition of the solvents during sample separation?

    <p>Gradient elution (B)</p> Signup and view all the answers

    What is the term for the part of the effluent that contains the eluted substance?

    <p>Eluate (D)</p> Signup and view all the answers

    What is the advantage of using smaller particle sizes in chromatography?

    <p>Increased theoretical plates (C)</p> Signup and view all the answers

    What is the definition of Retention Volume in chromatography?

    <p>The volume of mobile phase required to elute the solute components from the column (B)</p> Signup and view all the answers

    What is Dead Time in chromatography?

    <p>The time taken for the mobile phase to pass through the column without any interaction with the stationary phase (C)</p> Signup and view all the answers

    What is the purpose of using Uracil in reversed phase adsorption?

    <p>To measure the Dead Time and Volume of an unretained compound (C)</p> Signup and view all the answers

    What is the formula to calculate the Corrected Retention Time?

    <p>Retention Time - Dead Time (A)</p> Signup and view all the answers

    What is the relationship between Retention Volume and Dead Volume?

    <p>Retention Volume is equal to the difference between the Retention Volume and Dead Volume (D)</p> Signup and view all the answers

    Flashcards

    Plug of cotton purpose

    Prevents loss of stationary phase in column chromatography.

    Wet packing vs. Dry packing

    Wet packing makes a slurry of stationary phase with mobile phase, while dry packing doesn't.

    Dry packing disadvantage

    Non-uniform packing and air bubbles formation.

    Silica layer in wet loading

    Prevents distribution of sample to mobile phase.

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    Loading in column chromatography

    Adding the mixture to the top of the column.

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    Vertical glass cylindrical tube purpose

    Facilitates the flow of mobile phase in column chromatography.

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    Rotary evaporator purpose (dry loading)

    Removes solvent to get dry, free-flowing silica.

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    Air bubbles in column loading

    Prevent cracking of silica during sample loading.

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    Eluent

    The mobile phase used for separation.

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    Gradient elution

    Gradually changing solvent composition during separation.

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    Eluate

    Part of the effluent containing the eluted substance.

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    Smaller particle sizes in chromatography

    Increased theoretical plates, improving separation efficiency.

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    Retention Volume

    Volume of mobile phase to elute the solute.

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    Dead Time

    Time for mobile phase to pass without interaction.

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    Uracil in reversed phase

    Used to measure dead time and volume of unretained compound.

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    Corrected Retention Time

    Retention Time minus Dead Time.

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    Retention Volume vs. Dead Volume

    Retention Volume is the difference between the retention volume and the dead volume.

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