17 Questions
What is the main purpose of using a plug of cotton in column chromatography?
To prevent loss of the stationary phase
What is the difference between wet packing and dry packing in column chromatography?
Wet packing involves making a slurry of the stationary phase with the mobile phase
What is the disadvantage of dry packing in column chromatography?
Non-uniform packing and air bubbles formation
What is the purpose of adding a layer of silica to the top of the column during wet loading?
To prevent the distribution of the sample to the mobile phase
What is the term given to the process of adding the mixture to the top of the column in column chromatography?
Loading
What is the purpose of using a vertical glass cylindrical tube in column chromatography?
To facilitate the flow of the mobile phase
What is the primary purpose of using a rotary evaporator in the Dry Loading method?
To remove the solvent and obtain dry and free-flowing silica
Why is it important to ensure that there are no air bubbles in the column during sample loading?
To prevent cracking of the silica
What is the term for the mobile phase used to perform a separation?
Eluent
What is the result of gradually changing the composition of the solvents during sample separation?
Gradient elution
What is the term for the part of the effluent that contains the eluted substance?
Eluate
What is the advantage of using smaller particle sizes in chromatography?
Increased theoretical plates
What is the definition of Retention Volume in chromatography?
The volume of mobile phase required to elute the solute components from the column
What is Dead Time in chromatography?
The time taken for the mobile phase to pass through the column without any interaction with the stationary phase
What is the purpose of using Uracil in reversed phase adsorption?
To measure the Dead Time and Volume of an unretained compound
What is the formula to calculate the Corrected Retention Time?
Retention Time - Dead Time
What is the relationship between Retention Volume and Dead Volume?
Retention Volume is equal to the difference between the Retention Volume and Dead Volume
Test your understanding of column chromatography, a laboratory technique used to separate and purify components of a mixture. Learn how to use a glass column packed with a stationary phase and a liquid mobile phase to achieve separation. This tutorial is ideal for pharmacology students and researchers.
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