36 Questions
What is the purpose of attaching unique color dyes to ddNTPs?
To label and identify the terminated fragments
Which color dye is used to label ddATP?
Green
What is the color dye used to label ddGTP?
Black
What is the color dye used to label ddTTP?
Red
What is the color dye used to label ddCTP?
Blue
What is the purpose of gel/capillary electrophoresis in DNA sequencing?
To separate the fragments by size
What is the 3' end of a cDNA fragment?
The end with the unique color dye attached
What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?
To amplify the DNA sample
What information does the order in which the fragments separate during electrophoresis give?
The nucleotide sequence of each fragment
What is the purpose of ddNTPs in DNA sequencing?
To label and identify the terminated fragments
What is the difference between ddNTPs and regular dNTPs?
ddNTPs have a hydroxyl group on the 3' carbon, while regular dNTPs have a phosphate group on the 3' carbon
What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?
To label and identify the terminated fragments
What is the purpose of attaching unique color dyes to ddNTPs?
To label and identify the terminated fragments
Which color dye is used to label ddATP?
Green
What is the color dye used to label ddGTP?
Black
What is the color dye used to label ddTTP?
Red
What is the color dye used to label ddCTP?
Blue
What is the purpose of gel/capillary electrophoresis in DNA sequencing?
To separate the fragments by size
What is the 3' end of a cDNA fragment?
The end opposite to the unique color dye attached
What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?
To amplify the DNA sample
What information does the order in which the fragments separate during electrophoresis give?
The nucleotide sequence of each fragment
What is the purpose of ddNTPs in DNA sequencing?
To label and identify the terminated fragments
What is the difference between ddNTPs and regular dNTPs?
ddNTPs have a hydroxyl group on the 3' carbon, while regular dNTPs have a phosphate group on the 3' carbon
What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?
To label and identify the terminated fragments
What is the purpose of attaching unique color dyes to ddNTPs?
To label and identify the terminated fragments
Which color dye is used to label ddATP?
Green
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
To obtain the sequence
How are the DNA fragments separated in a DNA sequencing reaction?
Gel electrophoresis or capillary electrophoresis
What is the order in which the fragments separate during electrophoresis used for?
To obtain the sequence
Which type of DNA fragments are produced in a DNA sequencing reaction?
Single-stranded cDNA fragments
What is the purpose of ddCTP in a DNA sequencing reaction?
To label and identify the undamaged fragments
Which color dye is used to label ddTTP?
Red
What is the purpose of gel electrophoresis in a DNA sequencing reaction?
To separate the DNA fragments by size
Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
3' end
What is the purpose of ddGTP in a DNA sequencing reaction?
To label and identify the terminated fragments
Which color dye is used to label ddGTP?
Black
"Test your Knowledge on DNA Sequencing with Color-coded ddNTPs" - Take this quiz to learn about the unique color dyes attached to ddNTPs used for labeling and identifying DNA fragments. Challenge yourself to identify the different colors assigned to each ddNTP and understand how they aid in the sequencing process. Perfect for students and professionals in the field of molecular biology and genetics.
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