Color-coded ddNTPs

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What is the purpose of attaching unique color dyes to ddNTPs?

To mark the 5' end of each fragment

To label and identify the terminated fragments (correct)

To sequence the fragments

To produce single-stranded cDNA fragments

Which color dye is used to label ddATP?

Blue

Green (correct)

Black

Red

What is the color dye used to label ddGTP?

Red

Black (correct)

Green

Blue

What is the color dye used to label ddTTP?

Red Signup and view all the answers

What is the color dye used to label ddCTP?

Blue Signup and view all the answers

What is the purpose of gel/capillary electrophoresis in DNA sequencing?

To separate the fragments by size Signup and view all the answers

What is the 3' end of a cDNA fragment?

The end with the unique color dye attached Signup and view all the answers

What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?

To amplify the DNA sample Signup and view all the answers

What information does the order in which the fragments separate during electrophoresis give?

The nucleotide sequence of each fragment Signup and view all the answers

What is the purpose of ddNTPs in DNA sequencing?

To label and identify the terminated fragments Signup and view all the answers

What is the difference between ddNTPs and regular dNTPs?

ddNTPs have a hydroxyl group on the 3' carbon, while regular dNTPs have a phosphate group on the 3' carbon Signup and view all the answers

What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?

To label and identify the terminated fragments Signup and view all the answers

What is the purpose of attaching unique color dyes to ddNTPs?

To label and identify the terminated fragments Signup and view all the answers

Which color dye is used to label ddATP?

Green Signup and view all the answers

What is the color dye used to label ddGTP?

Black Signup and view all the answers

What is the color dye used to label ddTTP?

Red Signup and view all the answers

What is the color dye used to label ddCTP?

Blue Signup and view all the answers

What is the purpose of gel/capillary electrophoresis in DNA sequencing?

To separate the fragments by size Signup and view all the answers

What is the 3' end of a cDNA fragment?

The end opposite to the unique color dye attached Signup and view all the answers

What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?

To amplify the DNA sample Signup and view all the answers

What information does the order in which the fragments separate during electrophoresis give?

The nucleotide sequence of each fragment Signup and view all the answers

What is the purpose of ddNTPs in DNA sequencing?

To label and identify the terminated fragments Signup and view all the answers

What is the difference between ddNTPs and regular dNTPs?

ddNTPs have a hydroxyl group on the 3' carbon, while regular dNTPs have a phosphate group on the 3' carbon Signup and view all the answers

What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?

To label and identify the terminated fragments Signup and view all the answers

What is the purpose of attaching unique color dyes to ddNTPs?

To label and identify the terminated fragments Signup and view all the answers

Which color dye is used to label ddATP?

Green Signup and view all the answers

What is the significance of marking the 3’ end of each cDNA fragment with a dye?

To obtain the sequence Signup and view all the answers

How are the DNA fragments separated in a DNA sequencing reaction?

Gel electrophoresis or capillary electrophoresis Signup and view all the answers

What is the order in which the fragments separate during electrophoresis used for?

To obtain the sequence Signup and view all the answers

Which type of DNA fragments are produced in a DNA sequencing reaction?

Single-stranded cDNA fragments Signup and view all the answers

What is the purpose of ddCTP in a DNA sequencing reaction?

To label and identify the undamaged fragments Signup and view all the answers

Which color dye is used to label ddTTP?

Red Signup and view all the answers

What is the purpose of gel electrophoresis in a DNA sequencing reaction?

To separate the DNA fragments by size Signup and view all the answers

Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?

3' end Signup and view all the answers

What is the purpose of ddGTP in a DNA sequencing reaction?

To label and identify the terminated fragments Signup and view all the answers

Which color dye is used to label ddGTP?

Black Signup and view all the answers

Color-coded ddNTPs

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Questions and Answers

What is the purpose of attaching unique color dyes to ddNTPs?

Which color dye is used to label ddATP?

What is the color dye used to label ddGTP?

What is the color dye used to label ddTTP?

What is the color dye used to label ddCTP?

What is the purpose of gel/capillary electrophoresis in DNA sequencing?

What is the 3' end of a cDNA fragment?

What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?

What information does the order in which the fragments separate during electrophoresis give?

What is the purpose of ddNTPs in DNA sequencing?

What is the difference between ddNTPs and regular dNTPs?

What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?

What is the purpose of attaching unique color dyes to ddNTPs?

Which color dye is used to label ddATP?

What is the color dye used to label ddGTP?

What is the color dye used to label ddTTP?

What is the color dye used to label ddCTP?

What is the purpose of gel/capillary electrophoresis in DNA sequencing?

What is the 3' end of a cDNA fragment?

What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?

What information does the order in which the fragments separate during electrophoresis give?

What is the purpose of ddNTPs in DNA sequencing?

What is the difference between ddNTPs and regular dNTPs?

What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?

What is the purpose of attaching unique color dyes to ddNTPs?

Which color dye is used to label ddATP?

What is the significance of marking the 3’ end of each cDNA fragment with a dye?

How are the DNA fragments separated in a DNA sequencing reaction?

What is the order in which the fragments separate during electrophoresis used for?

Which type of DNA fragments are produced in a DNA sequencing reaction?

What is the purpose of ddCTP in a DNA sequencing reaction?

Which color dye is used to label ddTTP?

What is the purpose of gel electrophoresis in a DNA sequencing reaction?

Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?

What is the purpose of ddGTP in a DNA sequencing reaction?

Which color dye is used to label ddGTP?

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