Podcast
Questions and Answers
What is the purpose of attaching unique color dyes to ddNTPs?
What is the purpose of attaching unique color dyes to ddNTPs?
- To mark the 5' end of each fragment
- To label and identify the terminated fragments (correct)
- To sequence the fragments
- To produce single-stranded cDNA fragments
Which color dye is used to label ddATP?
Which color dye is used to label ddATP?
- Blue
- Green (correct)
- Black
- Red
What is the color dye used to label ddGTP?
What is the color dye used to label ddGTP?
- Red
- Black (correct)
- Green
- Blue
What is the color dye used to label ddTTP?
What is the color dye used to label ddTTP?
What is the color dye used to label ddCTP?
What is the color dye used to label ddCTP?
What is the purpose of gel/capillary electrophoresis in DNA sequencing?
What is the purpose of gel/capillary electrophoresis in DNA sequencing?
What is the 3' end of a cDNA fragment?
What is the 3' end of a cDNA fragment?
What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?
What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?
What information does the order in which the fragments separate during electrophoresis give?
What information does the order in which the fragments separate during electrophoresis give?
What is the purpose of ddNTPs in DNA sequencing?
What is the purpose of ddNTPs in DNA sequencing?
What is the difference between ddNTPs and regular dNTPs?
What is the difference between ddNTPs and regular dNTPs?
What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?
What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?
What is the purpose of attaching unique color dyes to ddNTPs?
What is the purpose of attaching unique color dyes to ddNTPs?
Which color dye is used to label ddATP?
Which color dye is used to label ddATP?
What is the color dye used to label ddGTP?
What is the color dye used to label ddGTP?
What is the color dye used to label ddTTP?
What is the color dye used to label ddTTP?
What is the color dye used to label ddCTP?
What is the color dye used to label ddCTP?
What is the purpose of gel/capillary electrophoresis in DNA sequencing?
What is the purpose of gel/capillary electrophoresis in DNA sequencing?
What is the 3' end of a cDNA fragment?
What is the 3' end of a cDNA fragment?
What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?
What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?
What information does the order in which the fragments separate during electrophoresis give?
What information does the order in which the fragments separate during electrophoresis give?
What is the purpose of ddNTPs in DNA sequencing?
What is the purpose of ddNTPs in DNA sequencing?
What is the difference between ddNTPs and regular dNTPs?
What is the difference between ddNTPs and regular dNTPs?
What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?
What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?
What is the purpose of attaching unique color dyes to ddNTPs?
What is the purpose of attaching unique color dyes to ddNTPs?
Which color dye is used to label ddATP?
Which color dye is used to label ddATP?
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
How are the DNA fragments separated in a DNA sequencing reaction?
How are the DNA fragments separated in a DNA sequencing reaction?
What is the order in which the fragments separate during electrophoresis used for?
What is the order in which the fragments separate during electrophoresis used for?
Which type of DNA fragments are produced in a DNA sequencing reaction?
Which type of DNA fragments are produced in a DNA sequencing reaction?
What is the purpose of ddCTP in a DNA sequencing reaction?
What is the purpose of ddCTP in a DNA sequencing reaction?
Which color dye is used to label ddTTP?
Which color dye is used to label ddTTP?
What is the purpose of gel electrophoresis in a DNA sequencing reaction?
What is the purpose of gel electrophoresis in a DNA sequencing reaction?
Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
What is the purpose of ddGTP in a DNA sequencing reaction?
What is the purpose of ddGTP in a DNA sequencing reaction?
Which color dye is used to label ddGTP?
Which color dye is used to label ddGTP?
