Color-coded ddNTPs
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Questions and Answers

What is the purpose of attaching unique color dyes to ddNTPs?

  • To mark the 5' end of each fragment
  • To label and identify the terminated fragments (correct)
  • To sequence the fragments
  • To produce single-stranded cDNA fragments

    • Which color dye is used to label ddATP?

  • Blue
  • Green (correct)
  • Black
  • Red

    • What is the color dye used to label ddGTP?

  • Red
  • Black (correct)
  • Green
  • Blue

    • What is the color dye used to label ddTTP?

    <p>Red</p> Signup and view all the answers

    What is the color dye used to label ddCTP?

    <p>Blue</p> Signup and view all the answers

    What is the purpose of gel/capillary electrophoresis in DNA sequencing?

    <p>To separate the fragments by size</p> Signup and view all the answers

    What is the 3' end of a cDNA fragment?

    <p>The end with the unique color dye attached</p> Signup and view all the answers

    What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?

    <p>To amplify the DNA sample</p> Signup and view all the answers

    What information does the order in which the fragments separate during electrophoresis give?

    <p>The nucleotide sequence of each fragment</p> Signup and view all the answers

    What is the purpose of ddNTPs in DNA sequencing?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    What is the difference between ddNTPs and regular dNTPs?

    <p>ddNTPs have a hydroxyl group on the 3' carbon, while regular dNTPs have a phosphate group on the 3' carbon</p> Signup and view all the answers

    What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    What is the purpose of attaching unique color dyes to ddNTPs?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    Which color dye is used to label ddATP?

    <p>Green</p> Signup and view all the answers

    What is the color dye used to label ddGTP?

    <p>Black</p> Signup and view all the answers

    What is the color dye used to label ddTTP?

    <p>Red</p> Signup and view all the answers

    What is the color dye used to label ddCTP?

    <p>Blue</p> Signup and view all the answers

    What is the purpose of gel/capillary electrophoresis in DNA sequencing?

    <p>To separate the fragments by size</p> Signup and view all the answers

    What is the 3' end of a cDNA fragment?

    <p>The end opposite to the unique color dye attached</p> Signup and view all the answers

    What is the purpose of producing single-stranded cDNA fragments in DNA sequencing?

    <p>To amplify the DNA sample</p> Signup and view all the answers

    What information does the order in which the fragments separate during electrophoresis give?

    <p>The nucleotide sequence of each fragment</p> Signup and view all the answers

    What is the purpose of ddNTPs in DNA sequencing?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    What is the difference between ddNTPs and regular dNTPs?

    <p>ddNTPs have a hydroxyl group on the 3' carbon, while regular dNTPs have a phosphate group on the 3' carbon</p> Signup and view all the answers

    What is the purpose of marking the 3' end of each fragment with a dye in DNA sequencing?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    What is the purpose of attaching unique color dyes to ddNTPs?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    Which color dye is used to label ddATP?

    <p>Green</p> Signup and view all the answers

    What is the significance of marking the 3’ end of each cDNA fragment with a dye?

    <p>To obtain the sequence</p> Signup and view all the answers

    How are the DNA fragments separated in a DNA sequencing reaction?

    <p>Gel electrophoresis or capillary electrophoresis</p> Signup and view all the answers

    What is the order in which the fragments separate during electrophoresis used for?

    <p>To obtain the sequence</p> Signup and view all the answers

    Which type of DNA fragments are produced in a DNA sequencing reaction?

    <p>Single-stranded cDNA fragments</p> Signup and view all the answers

    What is the purpose of ddCTP in a DNA sequencing reaction?

    <p>To label and identify the undamaged fragments</p> Signup and view all the answers

    Which color dye is used to label ddTTP?

    <p>Red</p> Signup and view all the answers

    What is the purpose of gel electrophoresis in a DNA sequencing reaction?

    <p>To separate the DNA fragments by size</p> Signup and view all the answers

    Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?

    <p>3' end</p> Signup and view all the answers

    What is the purpose of ddGTP in a DNA sequencing reaction?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    Which color dye is used to label ddGTP?

    <p>Black</p> Signup and view all the answers

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