Color-coded ddNTPs

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What is the purpose of attaching unique color dyes to ddNTPs?

To label and identify the terminated fragments (correct)

To prevent the fragments from separating

To enhance the amplification of fragments

To label and identify the undamaged fragments

Which color dye is used to label ddATP?

Black

Blue

Red

Green (correct)

What is the significance of marking the 3’ end of each cDNA fragment with a dye?

To distinguish between single-stranded and double-stranded DNA

To determine the length of each fragment

To obtain the sequence (correct)

To identify the 5' end of each fragment

How are the DNA fragments separated in a DNA sequencing reaction?

Gel electrophoresis or capillary electrophoresis Signup and view all the answers

What is the order in which the fragments separate during electrophoresis used for?

To obtain the sequence Signup and view all the answers

Which type of DNA fragments are produced in a DNA sequencing reaction?

Single-stranded cDNA fragments Signup and view all the answers

Which color dye is used to label ddTTP?

Red Signup and view all the answers

What is the purpose of gel electrophoresis in a DNA sequencing reaction?

To separate the DNA fragments by size Signup and view all the answers

Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?

3' end Signup and view all the answers

What is the purpose of ddGTP in a DNA sequencing reaction?

To label and identify the terminated fragments Signup and view all the answers

Which color dye is used to label ddGTP?

Black Signup and view all the answers

Color-coded ddNTPs

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Questions and Answers

What is the purpose of attaching unique color dyes to ddNTPs?

Which color dye is used to label ddATP?

What is the significance of marking the 3’ end of each cDNA fragment with a dye?

How are the DNA fragments separated in a DNA sequencing reaction?

What is the order in which the fragments separate during electrophoresis used for?

Which type of DNA fragments are produced in a DNA sequencing reaction?

Which color dye is used to label ddTTP?

What is the purpose of gel electrophoresis in a DNA sequencing reaction?

Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?

What is the purpose of ddGTP in a DNA sequencing reaction?

Which color dye is used to label ddGTP?

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