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Questions and Answers
What is the purpose of attaching unique color dyes to ddNTPs?
What is the purpose of attaching unique color dyes to ddNTPs?
- To label and identify the terminated fragments (correct)
- To prevent the fragments from separating
- To enhance the amplification of fragments
- To label and identify the undamaged fragments
Which color dye is used to label ddATP?
Which color dye is used to label ddATP?
- Black
- Blue
- Red
- Green (correct)
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
- To distinguish between single-stranded and double-stranded DNA
- To determine the length of each fragment
- To obtain the sequence (correct)
- To identify the 5' end of each fragment
How are the DNA fragments separated in a DNA sequencing reaction?
How are the DNA fragments separated in a DNA sequencing reaction?
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What is the order in which the fragments separate during electrophoresis used for?
What is the order in which the fragments separate during electrophoresis used for?
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Which type of DNA fragments are produced in a DNA sequencing reaction?
Which type of DNA fragments are produced in a DNA sequencing reaction?
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Which color dye is used to label ddTTP?
Which color dye is used to label ddTTP?
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What is the purpose of gel electrophoresis in a DNA sequencing reaction?
What is the purpose of gel electrophoresis in a DNA sequencing reaction?
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Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
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What is the purpose of ddGTP in a DNA sequencing reaction?
What is the purpose of ddGTP in a DNA sequencing reaction?
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Which color dye is used to label ddGTP?
Which color dye is used to label ddGTP?
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