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Questions and Answers
What is the purpose of attaching unique color dyes to ddNTPs?
What is the purpose of attaching unique color dyes to ddNTPs?
- To label and identify the terminated fragments (correct)
- To prevent the fragments from separating
- To enhance the amplification of fragments
- To label and identify the undamaged fragments
Which color dye is used to label ddATP?
Which color dye is used to label ddATP?
- Black
- Blue
- Red
- Green (correct)
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
What is the significance of marking the 3’ end of each cDNA fragment with a dye?
- To distinguish between single-stranded and double-stranded DNA
- To determine the length of each fragment
- To obtain the sequence (correct)
- To identify the 5' end of each fragment
How are the DNA fragments separated in a DNA sequencing reaction?
How are the DNA fragments separated in a DNA sequencing reaction?
What is the order in which the fragments separate during electrophoresis used for?
What is the order in which the fragments separate during electrophoresis used for?
Which type of DNA fragments are produced in a DNA sequencing reaction?
Which type of DNA fragments are produced in a DNA sequencing reaction?
Which color dye is used to label ddTTP?
Which color dye is used to label ddTTP?
What is the purpose of gel electrophoresis in a DNA sequencing reaction?
What is the purpose of gel electrophoresis in a DNA sequencing reaction?
Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?
What is the purpose of ddGTP in a DNA sequencing reaction?
What is the purpose of ddGTP in a DNA sequencing reaction?
Which color dye is used to label ddGTP?
Which color dye is used to label ddGTP?
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