Color-coded ddNTPs
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Questions and Answers

What is the purpose of attaching unique color dyes to ddNTPs?

  • To label and identify the terminated fragments (correct)
  • To prevent the fragments from separating
  • To enhance the amplification of fragments
  • To label and identify the undamaged fragments

    • Which color dye is used to label ddATP?

  • Black
  • Blue
  • Red
  • Green (correct)

    • What is the significance of marking the 3’ end of each cDNA fragment with a dye?

  • To distinguish between single-stranded and double-stranded DNA
  • To determine the length of each fragment
  • To obtain the sequence (correct)
  • To identify the 5' end of each fragment

    • How are the DNA fragments separated in a DNA sequencing reaction?

    <p>Gel electrophoresis or capillary electrophoresis</p> Signup and view all the answers

    What is the order in which the fragments separate during electrophoresis used for?

    <p>To obtain the sequence</p> Signup and view all the answers

    Which type of DNA fragments are produced in a DNA sequencing reaction?

    <p>Single-stranded cDNA fragments</p> Signup and view all the answers

    Which color dye is used to label ddTTP?

    <p>Red</p> Signup and view all the answers

    What is the purpose of gel electrophoresis in a DNA sequencing reaction?

    <p>To separate the DNA fragments by size</p> Signup and view all the answers

    Which end of the cDNA fragment is marked with a dye in a DNA sequencing reaction?

    <p>3' end</p> Signup and view all the answers

    What is the purpose of ddGTP in a DNA sequencing reaction?

    <p>To label and identify the terminated fragments</p> Signup and view all the answers

    Which color dye is used to label ddGTP?

    <p>Black</p> Signup and view all the answers

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