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Questions and Answers
What is the purpose of attaching unique color dyes to ddNTPs in DNA sequencing?
What is the purpose of attaching unique color dyes to ddNTPs in DNA sequencing?
- To label and identify the terminated fragments (correct)
- To label and identify the single-stranded cDNA fragments
- To label and identify the RNA fragments
- To label and identify the double-stranded cDNA fragments
Which color dye is attached to ddATP in DNA sequencing?
Which color dye is attached to ddATP in DNA sequencing?
- Blue
- Green (correct)
- Black
- Red
What is the significance of marking the 3' end of each cDNA fragment with a dye in DNA sequencing?
What is the significance of marking the 3' end of each cDNA fragment with a dye in DNA sequencing?
- To identify the length of each fragment
- To identify the 5' end of each fragment
- To identify the origin of the DNA sample
- To identify the sequence (correct)
How is the sequence obtained in DNA sequencing?
How is the sequence obtained in DNA sequencing?
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What is the order in which the fragments separate during electrophoresis in DNA sequencing?
What is the order in which the fragments separate during electrophoresis in DNA sequencing?
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What type of fragments are produced in DNA sequencing?
What type of fragments are produced in DNA sequencing?
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