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Color-coded ddNTPs

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Questions and Answers

What is the purpose of attaching unique color dyes to ddNTPs in DNA sequencing?

To label and identify the terminated fragments (correct)

To label and identify the single-stranded cDNA fragments

To label and identify the RNA fragments

To label and identify the double-stranded cDNA fragments

Which color dye is attached to ddATP in DNA sequencing?

Blue

Green (correct)

Black

Red

What is the significance of marking the 3' end of each cDNA fragment with a dye in DNA sequencing?

To identify the length of each fragment

To identify the 5' end of each fragment

To identify the origin of the DNA sample

To identify the sequence (correct)

How is the sequence obtained in DNA sequencing?

By gel/capillary electrophoresis Signup and view all the answers

What is the order in which the fragments separate during electrophoresis in DNA sequencing?

The order in which they were terminated Signup and view all the answers

What type of fragments are produced in DNA sequencing?

Single-stranded cDNA fragments Signup and view all the answers

Color-coded ddNTPs

Choose a study mode

Podcast

Questions and Answers

What is the purpose of attaching unique color dyes to ddNTPs in DNA sequencing?

Which color dye is attached to ddATP in DNA sequencing?

What is the significance of marking the 3' end of each cDNA fragment with a dye in DNA sequencing?

How is the sequence obtained in DNA sequencing?

What is the order in which the fragments separate during electrophoresis in DNA sequencing?

What type of fragments are produced in DNA sequencing?

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