Coagulase Test for Bacteria Identification
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Questions and Answers

What is the purpose of the coagulase test in identifying bacteria?

The coagulase test differentiates Staphylococcus aureus, which produces coagulase and causes agglutination, from other Staphylococcus species that do not.

In the slide coagulase test, what indicates a positive result?

A positive result is indicated by the agglutination or clumping of cocci within 5-10 seconds.

What materials are required to perform the slide coagulase test?

The materials required include a clean microscope slide, a pipette for adding plasma, an inoculating needle, and mammalian plasma.

What distinguishes the results of the positive and negative controls in the coagulase test?

<p>The positive control (Staphylococcus aureus) shows clumping while the negative control (Staphylococcus epidermidis) shows no clumping.</p> Signup and view all the answers

What role does mammalian plasma play in the coagulase test?

<p>Mammalian plasma serves as the reagent that reacts with the bacteria to demonstrate the presence of coagulase through agglutination.</p> Signup and view all the answers

The positive control organism used in the coagulase test is ______.

<p>Staphylococcus aureus</p> Signup and view all the answers

The coagulase test involves the use of citrated mammalian ______.

<p>plasma</p> Signup and view all the answers

A negative result in the slide coagulase test indicates ______ when using Staphylococcus epidermidis.

<p>no agglutination</p> Signup and view all the answers

The slide coagulase test can show positive agglutination within ______ seconds.

<p>5-10</p> Signup and view all the answers

In the coagulase test procedure, a small amount of growth is taken from a ______ plate.

<p>culture</p> Signup and view all the answers

Flashcards

Coagulase Test

A laboratory test used to detect the presence of coagulase enzyme in bacteria, specifically Staphylococcus.

Slide Coagulase Test

A method to detect coagulase enzyme using a microscope slide and plasma.

Positive Result (Slide)

Clumping of cocci (bacteria) within 5-10 seconds on the slide indicates coagulase presence.

Negative Result (Slide)

No clumping of cocci after 5-10 seconds on the slide signifies the absence of coagulase.

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Staphylococcus aureus

A bacterium that commonly tests positive for coagulase, showing clumping.

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Tube Coagulase Test

This method uses test tubes to detect the coagulase enzyme by observing clot formation in mammalian plasma.

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Coagulase Enzyme Function

The coagulase enzyme helps bacteria to clot blood plasma by converting fibrinogen into fibrin, forming small clots around bacteria.

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What is the purpose of using citrated plasma?

Citrated plasma is used because it's treated with citrate to prevent the natural clotting of the plasma, facilitating the detection of bacterial coagulase.

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What is a positive tube coagulase test?

A positive tube coagulase test shows the formation of a visible clot in the plasma within 24 hours, indicating the presence of coagulase enzyme.

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Why is it important to use controls?

Controls (positive and negative) are used to verify the test procedure and ensure accurate results. A positive control confirms that the test works, while a negative control ensures no false positive results.

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Study Notes

Coagulase Test Procedure

  • Equipment required: Safety cabinet, Bunsen burner, pipettes, small test tubes, glass slide, pointed glass rod
  • Reagents needed: Citrated mammalian plasma
  • Specimens: Isolated colonies of culture (samples A & B), positive control ( Staphylococcus aureus), negative control (e.g., Streptococcus spp. or S. epidermidis)

Slide Coagulase Test

  • Place bacterial growth from the culture plate onto a microscope slide
  • Add mammalian plasma and mix thoroughly with a needle
  • Agglutination (clumping) of cocci within 5-10 seconds indicates a positive result

Tube Coagulase Test (Method not detailed)

  • (Procedure not provided for this method)

Observation and Interpretation

  • Positive result: Agglutination or clumping of cocci within 5-10 seconds. This is indicative of Staphylococcus aureus.
  • Negative result: No agglutination or clumping. This typically indicates Staphylococcus epidermidis.

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Description

This quiz covers the procedure for performing the coagulase test, including equipment, reagents, and interpretation of results. Learn how to differentiate between Staphylococcus aureus and other species through agglutination. Perfect for microbiology students and laboratory technicians.

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