Podcast
Questions and Answers
What is the purpose of the coagulase test in identifying bacteria?
What is the purpose of the coagulase test in identifying bacteria?
The coagulase test differentiates Staphylococcus aureus, which produces coagulase and causes agglutination, from other Staphylococcus species that do not.
In the slide coagulase test, what indicates a positive result?
In the slide coagulase test, what indicates a positive result?
A positive result is indicated by the agglutination or clumping of cocci within 5-10 seconds.
What materials are required to perform the slide coagulase test?
What materials are required to perform the slide coagulase test?
The materials required include a clean microscope slide, a pipette for adding plasma, an inoculating needle, and mammalian plasma.
What distinguishes the results of the positive and negative controls in the coagulase test?
What distinguishes the results of the positive and negative controls in the coagulase test?
Signup and view all the answers
What role does mammalian plasma play in the coagulase test?
What role does mammalian plasma play in the coagulase test?
Signup and view all the answers
The positive control organism used in the coagulase test is ______.
The positive control organism used in the coagulase test is ______.
Signup and view all the answers
The coagulase test involves the use of citrated mammalian ______.
The coagulase test involves the use of citrated mammalian ______.
Signup and view all the answers
A negative result in the slide coagulase test indicates ______ when using Staphylococcus epidermidis.
A negative result in the slide coagulase test indicates ______ when using Staphylococcus epidermidis.
Signup and view all the answers
The slide coagulase test can show positive agglutination within ______ seconds.
The slide coagulase test can show positive agglutination within ______ seconds.
Signup and view all the answers
In the coagulase test procedure, a small amount of growth is taken from a ______ plate.
In the coagulase test procedure, a small amount of growth is taken from a ______ plate.
Signup and view all the answers
Study Notes
Coagulase Test Procedure
- Equipment required: Safety cabinet, Bunsen burner, pipettes, small test tubes, glass slide, pointed glass rod
- Reagents needed: Citrated mammalian plasma
- Specimens: Isolated colonies of culture (samples A & B), positive control ( Staphylococcus aureus), negative control (e.g., Streptococcus spp. or S. epidermidis)
Slide Coagulase Test
- Place bacterial growth from the culture plate onto a microscope slide
- Add mammalian plasma and mix thoroughly with a needle
- Agglutination (clumping) of cocci within 5-10 seconds indicates a positive result
Tube Coagulase Test (Method not detailed)
- (Procedure not provided for this method)
Observation and Interpretation
- Positive result: Agglutination or clumping of cocci within 5-10 seconds. This is indicative of Staphylococcus aureus.
- Negative result: No agglutination or clumping. This typically indicates Staphylococcus epidermidis.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
This quiz covers the procedure for performing the coagulase test, including equipment, reagents, and interpretation of results. Learn how to differentiate between Staphylococcus aureus and other species through agglutination. Perfect for microbiology students and laboratory technicians.
