Microbiology Enzyme Tests
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Questions and Answers

What is the process of microbial growth primarily responsible for?

  • Decreasing in size of population
  • Halting the growth of microbes
  • Reducing the number of viable cells
  • Synthesizing new cell constituents (correct)
  • During which phase of the microbial growth curve does the population growth stop and the curve becomes horizontal?

  • Lag phase
  • Stationary phase (correct)
  • Declining/death phase
  • Log/exponential phase
  • Which factor does NOT affect microbial growth according to the text?

  • Light intensity
  • Temperature
  • Toxic wastes
  • Nutrient availability (correct)
  • What is one of the measurements of microbial growth as mentioned in the text?

    <p>Measuring turbidity of culture</p> Signup and view all the answers

    In what process do bacterial protoplasm undergo compartmentalization and fragmentation forming minute bodies called gonidia?

    <p>Fragmentation</p> Signup and view all the answers

    What is the purpose of the Catalase test?

    <p>To test for the presence of catalase enzyme in an organism</p> Signup and view all the answers

    What does a positive result in the Oxidase test indicate?

    <p>Presence of cytochrome oxidase enzyme</p> Signup and view all the answers

    What is the purpose of the Urease test?

    <p>To demonstrate the production of urease enzyme</p> Signup and view all the answers

    What does a positive Indole test indicate?

    <p>Ability to degrade Tryptophan and produce Indole</p> Signup and view all the answers

    What does H2S production help identify in microorganisms?

    <p>Ability to produce H2S from decomposition of Sulphur compounds</p> Signup and view all the answers

    Study Notes

    Microbial Growth

    • Microbial growth is primarily responsible for decomposition and recycling of organic matter.

    Phases of Microbial Growth

    • During the stationary phase, the population growth stops and the curve becomes horizontal.

    Factors Affecting Microbial Growth

    • pH, temperature, oxygen, and nutrient availability are factors that affect microbial growth.

    Measurements of Microbial Growth

    • One of the measurements of microbial growth is the turbidity of a culture.

    Sporulation

    • In sporulation, bacterial protoplasm undergo compartmentalization and fragmentation, forming minute bodies called gonidia.

    Catalase Test

    • The purpose of the Catalase test is to detect the presence of catalase enzyme in microorganisms.

    Oxidase Test

    • A positive result in the Oxidase test indicates the presence of cytochrome oxidase in microorganisms.

    Urease Test

    • The purpose of the Urease test is to detect the presence of urease enzyme in microorganisms.

    Indole Test

    • A positive Indole test indicates the presence of tryptophanase enzyme in microorganisms.

    H2S Production

    • H2S production helps identify the presence of sulfate-reducing microorganisms.

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    Description

    This quiz covers the two common enzyme tests in microbiology: Catalase test and Coagulase test. Learn about how these tests are performed and what the results indicate.

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