Podcast
Questions and Answers
What is the purpose of supplementing the medium with 1-2% FCS if CHO cells are not doubling every 14-17 hours?
What is the purpose of supplementing the medium with 1-2% FCS if CHO cells are not doubling every 14-17 hours?
- To maintain the cells in a suspended state
- To enhance cell growth and division (correct)
- To induce differentiation of the CHO cells
- To inhibit cell proliferation
Which element in the CMV vectors is responsible for replication in bacterial cells?
Which element in the CMV vectors is responsible for replication in bacterial cells?
- f1 origin
- pre-pro-trypsin leader (PPT) sequence
- hGH polyA
- β-lactamase gene (correct)
What does the SV40 replication origin contribute to in SV40 replication permissive cells?
What does the SV40 replication origin contribute to in SV40 replication permissive cells?
- Stabilization of plasmids
- Inhibition of transcription
- Promotion of protein secretion
- Enhancement of DNA replication (correct)
Which component directs the secretion of fusion proteins into the culture medium for purification?
Which component directs the secretion of fusion proteins into the culture medium for purification?
What is the function of the hCMV promoter regulatory region in the mammalian expression system?
What is the function of the hCMV promoter regulatory region in the mammalian expression system?
Why are CMV vectors favorable for expressing proteins in mammalian cells?
Why are CMV vectors favorable for expressing proteins in mammalian cells?
In the context of heterologous protein expression, what is the function of the lacI binding site in the lac operon control elements?
In the context of heterologous protein expression, what is the function of the lacI binding site in the lac operon control elements?
What cellular component is crucial for the production of large, complex glycosylated polypeptides in mammalian cell expression systems?
What cellular component is crucial for the production of large, complex glycosylated polypeptides in mammalian cell expression systems?
What modification is necessary to induce constitutive expression in an un-induced state using the lac operon control elements?
What modification is necessary to induce constitutive expression in an un-induced state using the lac operon control elements?
Which component is responsible for the stabilization of plasmids used in bacterial cells for heterologous protein expression?
Which component is responsible for the stabilization of plasmids used in bacterial cells for heterologous protein expression?
What characteristic makes Chinese Hamster Overy (CHO) cells a preferred host cell for mammalian expression systems?
What characteristic makes Chinese Hamster Overy (CHO) cells a preferred host cell for mammalian expression systems?
Which element of the lac operon control system directly affects the expression of the gene of interest in bacterial cells?
Which element of the lac operon control system directly affects the expression of the gene of interest in bacterial cells?
What is the primary control point of the CMV promoter?
What is the primary control point of the CMV promoter?
Which cell type shows a relatively high level of gene expression with the CMV promoter?
Which cell type shows a relatively high level of gene expression with the CMV promoter?
What factor is crucial for the tight control of growth conditions when using the CMV enhancer sequence?
What factor is crucial for the tight control of growth conditions when using the CMV enhancer sequence?
Which transcription factor has a strong preference for the CMV enhancer sequence?
Which transcription factor has a strong preference for the CMV enhancer sequence?
What type of cells show low levels of gene expression with the CMV promoter?
What type of cells show low levels of gene expression with the CMV promoter?
What is one disadvantage associated with the CMV enhancer sequence?
What is one disadvantage associated with the CMV enhancer sequence?
What is one potential issue with using the lac operon control elements for heterologous protein expression?
What is one potential issue with using the lac operon control elements for heterologous protein expression?
What is a characteristic of the T7 RNA Polymerase in the context of heterologous protein expression?
What is a characteristic of the T7 RNA Polymerase in the context of heterologous protein expression?
What is the main role of the lac repressor in the lac operon system?
What is the main role of the lac repressor in the lac operon system?
Why is it important to remove the lacZYA genes when using the lac operon for heterologous protein expression?
Why is it important to remove the lacZYA genes when using the lac operon for heterologous protein expression?
Which statement accurately describes the role of Galactoside permease (lacY) in the lac operon system?
Which statement accurately describes the role of Galactoside permease (lacY) in the lac operon system?
Why is a more tightly controlled promoter-expression-cassette needed when using the lac operon for heterologous protein expression?
Why is a more tightly controlled promoter-expression-cassette needed when using the lac operon for heterologous protein expression?
What is the main purpose of using different restriction enzymes to cut the donor and receiver DNA when cloning a gene?
What is the main purpose of using different restriction enzymes to cut the donor and receiver DNA when cloning a gene?
In the context of biotechnology, what is the role of T7 lysozyme in host cells like E. coli?
In the context of biotechnology, what is the role of T7 lysozyme in host cells like E. coli?
Which type of promoter is commonly used in eukaryotic expression vectors for strong gene expression?
Which type of promoter is commonly used in eukaryotic expression vectors for strong gene expression?
Why is orientation important when cloning genes using different restriction enzymes?
Why is orientation important when cloning genes using different restriction enzymes?
What is one advantage of using mammalian cells as an expression host over bacterial or yeast cells?
What is one advantage of using mammalian cells as an expression host over bacterial or yeast cells?
Why is it necessary to choose the appropriate expression format at the beginning of cloning work in biotechnology?
Why is it necessary to choose the appropriate expression format at the beginning of cloning work in biotechnology?
What are the major disadvantages of using inducible expression systems in biotechnology?
What are the major disadvantages of using inducible expression systems in biotechnology?
Why are stable inducible mammalian expression vectors considered the 'holy grail' for industrial recombinant protein expression?
Why are stable inducible mammalian expression vectors considered the 'holy grail' for industrial recombinant protein expression?
What is a concern associated with stable integration of genes into the genome in biotechnology?
What is a concern associated with stable integration of genes into the genome in biotechnology?
What are some examples of inducible systems used in biotechnology and their respective inducers?
What are some examples of inducible systems used in biotechnology and their respective inducers?
Why is serum batch-to-batch variability a concern in modulating enhancers in biotechnology?
Why is serum batch-to-batch variability a concern in modulating enhancers in biotechnology?
