Clinical Laboratory Safety and Procedures Quiz

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Questions and Answers

What is the primary function of type 3 water in a clinical laboratory?

Rinsing glassware and calibrating thermometers.
Maintaining specific temperatures in incubators and freezers.
Providing feed water to type 1 systems and cell culture incubators.
Filling autoclaves, heating baths, and humidifiers. (correct)

Which of the following best describes a solute?

A biologic fluid.
A substance that is dissolved in a liquid. (correct)
A type of solution.
The liquid in which a substance is dissolved.

What is the standard temperature of calibration mentioned for Class A glassware?

20 °C (correct)
0 °C
37 °C
25 °C

Which of the following is NOT a characteristic of a solution?

Temperature. (B) Signup and view all the answers

If a temperature is measured as 68°F, what is the equivalent temperature in Celsius?

20°C (D) Signup and view all the answers

If a clinical laboratory requires a temperature of 298K for a procedure, what would be the equivalent temperature in Celsius?

25 °C (B) Signup and view all the answers

Which type of thermometer is being replaced due to environmental concerns?

Liquid-in-glass thermometer containing mercury (A) Signup and view all the answers

Why is it important for clinical thermometers to be calibrated?

To ensure accurate temperature measurements by referencing an NIST certified thermometer (B) Signup and view all the answers

What is the primary purpose of a chemical fume hood?

To contain and expel noxious and hazardous fumes. (A) Signup and view all the answers

What is a critical factor to consider when operating a fume hood?

Establishing and marking a maximum operating sash height. (A) Signup and view all the answers

How should the ventilation of a fume hood be evaluated?

By measuring face velocity with a calibrated meter. (C) Signup and view all the answers

What is the recommended range for face velocity at the opening of a fume hood?

100 to 120 feet per minute. (D) Signup and view all the answers

When should the face velocity of a fume hood be measured?

Periodically. (A) Signup and view all the answers

What is the primary purpose of safety glasses, goggles, or visors in a laboratory?

To prevent splashing into the eyes and face. (C) Signup and view all the answers

Why are contact lenses not considered appropriate eye protection in the lab?

They do not offer splash or impact protection. (C) Signup and view all the answers

What is the purpose of an Exposure Control Plan regarding bloodborne pathogens?

To minimize the risk of blood or other potentially infectious material exposure. (B) Signup and view all the answers

What is the primary hazard associated with centrifuging biologic specimens?

The creation of finely dispersed aerosols that may contain infectious agents. (A) Signup and view all the answers

What is a recommended practice to minimize the risk of aerosol transmission when working with a standard centrifuge?

Wait several minutes after completion of the spin before opening the lid. (D) Signup and view all the answers

What is the most suitable alternative when a standard centrifuge is not ideal?

A sealed-cup centrifuge to prevent aerosol leaks. (D) Signup and view all the answers

What is the first action you should take when a spill occurs?

Alert others in the area about the spill. (D) Signup and view all the answers

When dealing with a spill that involves broken glass in the lab, which method of cleanup is recommended?

Use mechanical devices to pick up broken glass before other procedures. (A) Signup and view all the answers

Which of these is recommended for absorbing liquid spills?

Paper towels, gauze pads, or tissue. (B) Signup and view all the answers

After absorbing a spill, what is the next step to ensure the area is thoroughly cleaned?

Clean the area with an aqueous detergent. (A) Signup and view all the answers

According to the text which fire extinguisher is recommended for use in computer equipment areas?

Halogenated hydrocarbon extinguisher. (B) Signup and view all the answers

What is a primary characteristic of reactive chemicals?

They spontaneously ignite or explode or emit gases under specific conditions. (C) Signup and view all the answers

Which of the following actions should be taken first in the event of a chemical spill?

Assist or evacuate personnel from the area. (A) Signup and view all the answers

Which type of glove material is NOT acceptable for phlebotomy procedures?

All of the provided options are acceptable. (A) Signup and view all the answers

What is the most commonly used antiseptic for routine blood collection?

70% isopropyl alcohol (B) Signup and view all the answers

How does ambient temperature affect the draw volume of evacuated blood collection tubes?

Higher temperatures result in a reduced draw volume. (A) Signup and view all the answers

How does high altitude affect the draw volume of evacuated blood collection tubes?

High altitude leads to a lower draw volume. (C) Signup and view all the answers

Which of the following is NOT listed as an environmental factor associated with evacuated blood collection tubes?

Air pressure (A) Signup and view all the answers

What precaution should be taken when using disposable gloves for phlebotomy?

Ensure the gloves are disposable and non-sterile. (B) Signup and view all the answers

When using tubes with clot activators, what is the approximate clotting time?

5 minutes (B) Signup and view all the answers

Which of the following is the first step in sample collection?

Proper patient identification (C) Signup and view all the answers

A phlebotomist is having trouble finding an appropriate vein. After attempting the median cubital, what is the second vein of choice?

Cephalic Vein (B) Signup and view all the answers

Which type of specimen contains both plasma and cells?

Whole blood (D) Signup and view all the answers

What type of tube is typically used first in the Evacuated Tube System (ETS) order of draw?

Blood culture tube (B) Signup and view all the answers

In a dermal puncture, which of the following tubes is collected after the blood smear/slide?

EDTA tubes (A) Signup and view all the answers

How long does it take for a plain red-stoppered tube with no additives to clot completely?

60 minutes (D) Signup and view all the answers

Why are tubes containing gels not used for blood bank (BB) or immunological testing?

They may interfere with immunologic reactions (B) Signup and view all the answers

What is the primary function of the bevel on a phlebotomy needle?

To facilitate smooth insertion into the skin and vein. (B) Signup and view all the answers

Which needle gauge is considered standard for most routine adult antecubital venipunctures?

21-gauge (C) Signup and view all the answers

What is the recommended maximum time for tourniquet application during blood collection?

1 minute (60 seconds) (D) Signup and view all the answers

What ratio of blood to anticoagulant is required when using a light blue-top tube for coagulation testing?

9:1 (C) Signup and view all the answers

Which type of tube is primarily used for glucose measurements due to its antiglycolytic properties?

Gray-top tube (D) Signup and view all the answers

What is the mechanism of action of heparin as an anticoagulant?

It accelerates the action of antithrombin III (D) Signup and view all the answers

For which specific purpose are black-top tubes used?

Westergren ESR (D) Signup and view all the answers

Which of the following is a common cause of erroneous test results due to improper phlebotomy technique?

Prolonged tourniquet application results in hemoconcentration (C) Signup and view all the answers

What does the term 'lumen' refer to when discussing needles?

The internal space inside the needle (D) Signup and view all the answers

What is the effect of using a blood pressure cuff as a tourniquet, and what is the recommended pressure?

To provide a similar pressure to a standard tourniquet, 40 to 60 mmHg (B) Signup and view all the answers

What is the main purpose of the clot activator in red/gray- and gold-top tubes (SSTs)?

To accelerate the rate of clot formation (D) Signup and view all the answers

In obese patients, what is the recommended range for blood pressure cuff tourniquet pressure?

40 mmHg (B) Signup and view all the answers

What is the primary purpose of using multiple sample needles in the Evacuated Tube System (ETS)?

To enable multiple tubes to be filled from one venipuncture (D) Signup and view all the answers

What is the significance of the thixotropic gel found in red/gray- and gold-top SST tubes?

To separate serum from cellular components, potentially causing falsely lowered results with some drugs (D) Signup and view all the answers

Why is the use of sodium heparin preferred over lithium heparin for certain tests?

Because sodium heparin does not affect the levels of trace elements (D) Signup and view all the answers

Flashcards

Solute

The substance that gets dissolved into a liquid.

Solvent

The liquid that dissolves the solute.