Podcast
Questions and Answers
What is genetic engineering primarily concerned with?
What is genetic engineering primarily concerned with?
- Extracting genes from non-living organisms
- Producing genetically identical organisms
- Increasing the genetic diversity of a species
- Manipulating genes in living organisms (correct)
Which process allows for the expression of a transferred gene in a recipient organism?
Which process allows for the expression of a transferred gene in a recipient organism?
- Gene synthesis
- Transformation (correct)
- Gene silencing
- Genetic modification
What type of DNA is produced when genes from different organisms are combined?
What type of DNA is produced when genes from different organisms are combined?
- Recombinant DNA (correct)
- Mitochondrial DNA
- Genomic DNA
- Transgenic DNA
Why is human insulin produced in genetically modified bacteria rather than extracted from animals?
Why is human insulin produced in genetically modified bacteria rather than extracted from animals?
What component of mRNA is essential for synthesizing the insulin gene?
What component of mRNA is essential for synthesizing the insulin gene?
What differentiates prokaryotic genes from eukaryotic genes?
What differentiates prokaryotic genes from eukaryotic genes?
What is a significant outcome of gene therapy?
What is a significant outcome of gene therapy?
Which of the following best describes the role of reverse transcriptase in genetic technology?
Which of the following best describes the role of reverse transcriptase in genetic technology?
What is the purpose of adding sticky ends to DNA molecules?
What is the purpose of adding sticky ends to DNA molecules?
Which enzyme is primarily responsible for copying DNA during PCR?
Which enzyme is primarily responsible for copying DNA during PCR?
What characteristic of plasmids makes them suitable as vectors for gene transfer?
What characteristic of plasmids makes them suitable as vectors for gene transfer?
What is the primary function of the polymerase chain reaction (PCR)?
What is the primary function of the polymerase chain reaction (PCR)?
What role does DNA ligase play in the insertion of the insulin gene into the plasmid?
What role does DNA ligase play in the insertion of the insulin gene into the plasmid?
What method is used to identify E. coli that have successfully taken up plasmids?
What method is used to identify E. coli that have successfully taken up plasmids?
Why is Taq polymerase used in PCR?
Why is Taq polymerase used in PCR?
Which of the following is NOT a step in cloning DNA using plasmids?
Which of the following is NOT a step in cloning DNA using plasmids?
What role do restriction enzymes play in gel electrophoresis?
What role do restriction enzymes play in gel electrophoresis?
Why were antibiotic resistance genes introduced alongside the insulin gene in plasmids?
Why were antibiotic resistance genes introduced alongside the insulin gene in plasmids?
How does the size of DNA fragments affect their movement during gel electrophoresis?
How does the size of DNA fragments affect their movement during gel electrophoresis?
What occurs during the fermentation process after bacteria have taken up the plasmids?
What occurs during the fermentation process after bacteria have taken up the plasmids?
What is the purpose of primers in the PCR process?
What is the purpose of primers in the PCR process?
What is the proteome?
What is the proteome?
What happens to DNA when an electrical potential difference is applied during gel electrophoresis?
What happens to DNA when an electrical potential difference is applied during gel electrophoresis?
What causes the different speeds of DNA fragments during gel electrophoresis?
What causes the different speeds of DNA fragments during gel electrophoresis?
How can knowing DNA sequences of different Plasmodium species be beneficial?
How can knowing DNA sequences of different Plasmodium species be beneficial?
Which DNA alteration methods are possible with CRISPR-Cas9 technology?
Which DNA alteration methods are possible with CRISPR-Cas9 technology?
What is an advantage of insulin produced by genetically engineered E. coli?
What is an advantage of insulin produced by genetically engineered E. coli?
Which of the following proteins is produced by GM organisms for treating haemophilia?
Which of the following proteins is produced by GM organisms for treating haemophilia?
What role does AI play in the development of medical proteins?
What role does AI play in the development of medical proteins?
What is a potential future application of genetic databases in medicine?
What is a potential future application of genetic databases in medicine?
How does understanding the proteome contribute to health and metabolism studies?
How does understanding the proteome contribute to health and metabolism studies?
Which of the following statements about the genetic code is true?
Which of the following statements about the genetic code is true?
What type of reproduction do bacteria primarily undergo?
What type of reproduction do bacteria primarily undergo?
Why is a promoter important in genetic engineering?
Why is a promoter important in genetic engineering?
What is the role of marker genes in genetic engineering?
What is the role of marker genes in genetic engineering?
What is a concern associated with using antibiotic resistance genes as markers?
What is a concern associated with using antibiotic resistance genes as markers?
What technology is associated with gene editing for correcting specific DNA sequences?
What technology is associated with gene editing for correcting specific DNA sequences?
What is the first step in the CRISPR-Cas9 gene editing process?
What is the first step in the CRISPR-Cas9 gene editing process?
Where do the genes coding for green fluorescent protein (GFP) originate from?
Where do the genes coding for green fluorescent protein (GFP) originate from?
What does the presence of GFP indicate in genetically modified bacteria?
What does the presence of GFP indicate in genetically modified bacteria?
What is the primary reason for the limited supply of insulin from animal sources?
What is the primary reason for the limited supply of insulin from animal sources?
Which of the following is a method of genetic screening?
Which of the following is a method of genetic screening?
What does preimplantation genetic diagnosis involve?
What does preimplantation genetic diagnosis involve?
What ethical dilemma might arise from genetic screening in pregnant women?
What ethical dilemma might arise from genetic screening in pregnant women?
How can genetic screening benefit individuals with a family history of Huntington's disease?
How can genetic screening benefit individuals with a family history of Huntington's disease?
What is one possible outcome for someone who has the BRCA1 or BRCA2 alleles?
What is one possible outcome for someone who has the BRCA1 or BRCA2 alleles?
What role does a genetic counselor play in genetic screening?
What role does a genetic counselor play in genetic screening?
Which of the following describes a common concern regarding harvesting insulin from animals?
Which of the following describes a common concern regarding harvesting insulin from animals?
Flashcards
Genetic Engineering
Genetic Engineering
The manipulation of genes in living organisms. This involves inserting genes from one organism into another, potentially from different species. Genes can also be artificially synthesized and inserted.
Recombinant DNA
Recombinant DNA
DNA created by combining sequences from different organisms. This is achieved through genetic engineering techniques.
What is the purpose of genetic engineering?
What is the purpose of genetic engineering?
To introduce new traits, repair genetic defects, produce useful products, or study gene function.
What is insulin?
What is insulin?
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What happens in type I diabetes?
What happens in type I diabetes?
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What is reverse transcriptase?
What is reverse transcriptase?
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What is cDNA?
What is cDNA?
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What is the difference between eukaryotic and prokaryotic genes?
What is the difference between eukaryotic and prokaryotic genes?
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cDNA
cDNA
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Sticky ends
Sticky ends
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Restriction endonuclease
Restriction endonuclease
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Plasmid
Plasmid
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Vector
Vector
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Antibiotic resistance gene
Antibiotic resistance gene
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Fermenter
Fermenter
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Genetically Modified Bacteria
Genetically Modified Bacteria
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Promoter
Promoter
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What is the role of the promoter in genetically modified organisms?
What is the role of the promoter in genetically modified organisms?
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Marker Genes
Marker Genes
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What is the concern with using antibiotic resistance genes as markers?
What is the concern with using antibiotic resistance genes as markers?
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CRISPR-Cas9 technology
CRISPR-Cas9 technology
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Guide RNA
Guide RNA
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Cas9 enzyme
Cas9 enzyme
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CRISPR-Cas9
CRISPR-Cas9
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What can CRISPR-Cas9 do?
What can CRISPR-Cas9 do?
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Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)
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What does PCR rely on?
What does PCR rely on?
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Primer in PCR
Primer in PCR
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Taq polymerase
Taq polymerase
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Gel Electrophoresis
Gel Electrophoresis
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Restriction enzymes
Restriction enzymes
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Proteome
Proteome
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Genetic Databases
Genetic Databases
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Drug Development (with Databases)
Drug Development (with Databases)
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Disease Susceptibility
Disease Susceptibility
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Protein Modelling
Protein Modelling
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Gene Function
Gene Function
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Species Classification
Species Classification
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Human Protein Production (GM)
Human Protein Production (GM)
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Genetic Screening
Genetic Screening
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Carrier
Carrier
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Preimplantation Genetic Diagnosis (PGD)
Preimplantation Genetic Diagnosis (PGD)
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Prenatal Testing
Prenatal Testing
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Huntington's Disease
Huntington's Disease
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BRCA1/BRCA2 Alleles
BRCA1/BRCA2 Alleles
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Genetic Counselor
Genetic Counselor
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Ethical Issues in Genetic Screening
Ethical Issues in Genetic Screening
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Study Notes
Classification, Biodiversity and Conservation
- Biologists classify organisms based on their evolutionary relationships.
- The smallest classification group is the species.
- Defining species can be complex. Multiple species concepts exist.
- The biological species concept defines species based on interbreeding and fertile offspring.
- The biological species concept may not apply to asexual organisms or extinct species.
- The morphological species concept classifies organisms by shared physical characteristics.
- This can be problematic because similar-looking organisms may not be able to interbreed.
- The ecological species concept classifies organisms based on their ecological niches.
- Organisms in the same niche share similar ecological roles and resources.
Biodiversity
- Biodiversity is the variety of ecosystems, habitats, species, and genotypes in an area.
- An ecosystem includes all the living and non-living components in a defined area.
- Each species within an ecosystem plays a role, known as a niche.
- Niches are defined by factors like nutrition, food web position, and interaction with the environment.
- Biodiversity measurements can focus on ecosystems, species, and genetic variation.
- Species richness is the number of different species, reflecting biodiversity.
- Sampling techniques, like quadrats and transects, can assess biodiversity.
- Frame quadrats sample organisms within defined area samples
Extinction
- Species extinction is a natural process.
- Mass extinctions are events that involve the loss of many species.
- Factors that contribute to extinctions include climate change, competition, and habitat degradation.
Conservation
- Conservation aims to maintain or increase biodiversity in a given area.
- Extinction is a threat impacting biodiversity.
- Reasons for maintaining biodiversity include ecosystem stability and human dependency on species.
Endangered Species
- Species are endangered when their numbers have fallen to levels threatening their long-term survival.
- Factors like hunting and habitat loss are among the main causes of endangered species.
- Conservation efforts, like national parks, captive breeding programs and assisted reproduction, are used to maintain or enhance endangered species populations.
Gene Technology
- Genetic engineering manipulates genes within organisms.
- Genes can be transferred between species.
- Gene technology allows the production of recombinant DNA.
- Recombinant DNA is used to create organisms producing desired traits or proteins. Examples include insulin production, or solving genetic diseases.
- Techniques like Polymerase Chain Reaction (PCR) and Gel Electrophoresis are important aspects of the process.
- Gene editing is an advanced technique enabling precise alterations to DNA sequences.
- Ethical concerns arise from gene technology's application, including potential impacts on ecosystems and organisms.
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