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Chromatography TLC Plate Analysis
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Chromatography TLC Plate Analysis

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Questions and Answers

What percentage of the TLC plate is visible?

  • 36%
  • 41% (correct)
  • 46%
  • 31%
  • What is the code 'Vodafone 36 Vodafone 46 Ο Δ' referring to on the TLC plate?

  • Brands (correct)
  • Chemical compositions
  • Experimental procedures
  • Temperature conditions
  • What does the number '8173834' represent in SDOC-AABC8173834...?

  • Temperature reading
  • Unique identifier (correct)
  • Timestamp
  • Laboratory location
  • At what position is the SDOC-AABC8173834... located on the TLC plate?

    <p>Middle</p> Signup and view all the answers

    From which points can the TLC plate be viewed?

    <p>Top and bottom</p> Signup and view all the answers

    What is a recommended technique to keep spots small and compact during solvent evaporation?

    <p>Applying the sample all at once</p> Signup and view all the answers

    Why is it advised to use cold or hot air during solvent evaporation?

    <p>To evaporate the solvent</p> Signup and view all the answers

    If a sample is applied all at once without any drying time in between, what might happen?

    <p>The spots will diffuse</p> Signup and view all the answers

    What could be a consequence of not allowing any drying time between sample applications?

    <p>Increased risk of spot diffusion</p> Signup and view all the answers

    In what way does applying a sample in several portions impact the final outcome?

    <p>It helps in keeping the spots small and compact</p> Signup and view all the answers

    What kind of stationary phases are typically used for achieving good separation?

    <p>Polar</p> Signup and view all the answers

    Which type of mobile phases is recommended for achieving good separation?

    <p>Low polarity</p> Signup and view all the answers

    What is a key characteristic of the stationary phases used for achieving good separation?

    <p>Fairly polar</p> Signup and view all the answers

    Which type of mobile phase would likely not be effective for achieving good separation?

    <p>Highly polar</p> Signup and view all the answers

    How does using hexane as a mobile phase contribute to separation efficiency?

    <p>By maintaining low polarity</p> Signup and view all the answers

    What is the primary reason for competition in surface adsorption chromatography?

    <p>For the stationary phase adsorption sites</p> Signup and view all the answers

    What factor determines the choice of the stationary phase?

    <p>Polarity of the target solutes</p> Signup and view all the answers

    In surface adsorption chromatography, which components compete for adsorption sites?

    <p>Materials to be separated and the stationary phase</p> Signup and view all the answers

    What role does the mobile phase play in surface adsorption chromatography?

    <p>It facilitates separation by competing for sites</p> Signup and view all the answers

    Why is it important to consider the polarity of the target solutes in chromatography?

    <p>To optimize the interaction between solutes and stationary phase</p> Signup and view all the answers

    How does the polarity of the target solutes influence the choice of stationary phase?

    <p>It impacts how well solutes are retained and separated</p> Signup and view all the answers

    How does competition for adsorption sites impact the efficiency of surface adsorption chromatography?

    <p>It minimizes the resolution of peaks</p> Signup and view all the answers

    In chromatography, what role does the polarity of target solutes play in column efficiency?

    <p>Improves peak resolution</p> Signup and view all the answers

    Which factor determines the success of separation in surface adsorption chromatography?

    <p>The strength of interaction between phases</p> Signup and view all the answers

    What can happen if the polarity of the stationary phase does not match that of the target solutes?

    <p>Decreased retention of solutes</p> Signup and view all the answers

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