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Questions and Answers
What is the principle of TLC?
What happens to a less polar compound in TLC?
How are individual separated compounds visualized in TLC?
What is used as the solid phase in TLC?
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How is Rƒ (Retardation Factor) value calculated in TLC?
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What is the main purpose of using Thin Layer Chromatography (TLC)?
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What is the role of the stationary phase in Thin Layer Chromatography (TLC)?
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Which material can be used as the stationary phase in Thin Layer Chromatography (TLC)?
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What is the mobile phase in Thin Layer Chromatography (TLC)?
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What can Thin Layer Chromatography (TLC) be used to determine?
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Study Notes
Principle of TLC
- The principle of TLC is based on the difference in the partitioning behavior of the components of a mixture between the stationary phase and the mobile phase.
Separation and Visualization
- A less polar compound moves faster and farther up the TLC plate, while a more polar compound moves slower and stays closer to the starting point.
- Individual separated compounds are visualized in TLC by using a chemical reagent or by exposing the plate to iodine vapor or ultraviolet light.
Stationary Phase
- The solid phase used in TLC is usually a thin layer of silica gel or alumina on a glass plate or plastic sheet.
- The stationary phase is responsible for separating the components of a mixture based on their polarity.
Rƒ (Retardation Factor) Value
- The Rƒ value is calculated by dividing the distance traveled by the compound by the distance traveled by the solvent front.
Main Purpose of TLC
- The main purpose of using TLC is to identify, separate, and quantify the components of a mixture.
Role of Stationary Phase
- The role of the stationary phase is to separate the components of a mixture based on their polarity, allowing them to be visualized and characterized.
Stationary Phase Materials
- Silica gel, alumina, and cellulose can be used as the stationary phase in TLC.
Mobile Phase
- The mobile phase in TLC is a solvent that moves up the plate, carrying the components of the mixture with it.
Applications of TLC
- TLC can be used to determine the purity of a substance, identify the components of a mixture, and monitor the progress of a reaction.
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Description
Explore the fundamentals of Thin Layer Chromatography (TLC) in the context of Biotechnology Chapter 2. Learn about the principles and applications of TLC and its significance in biotechnological processes.