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Questions and Answers
What is the purpose of gently blowing onto the applied spot until the solvent evaporates in TLC analysis?
What is the purpose of gently blowing onto the applied spot until the solvent evaporates in TLC analysis?
- To prevent contamination of the sample
- To minimize spot spreading or enlargement (correct)
- To speed up the process of solvent evaporation
- To aid in the separation of components in the sample
Why is it essential to avoid scratching the surface of the TLC plate during sample application?
Why is it essential to avoid scratching the surface of the TLC plate during sample application?
- To speed up the analysis process
- To prevent mixing of the samples
- To ensure accurate calculation of Rf values
- To avoid damaging the stationary phase (correct)
What is the purpose of marking the final position of the solvent front using a pencil after removing the TLC plate from the tank?
What is the purpose of marking the final position of the solvent front using a pencil after removing the TLC plate from the tank?
- To help visualize the spots on the plate
- To track the movement of the solvent front
- To determine the Rf values of the samples
- To guide in calculating the distance traveled by the solvent (correct)
Why is it recommended to use a designated capillary tube for each reference standard during sample application?
Why is it recommended to use a designated capillary tube for each reference standard during sample application?
In TLC analysis, why should the mobile phase only come up to between 0.5 and 1 cm from the bottom of the plate?
In TLC analysis, why should the mobile phase only come up to between 0.5 and 1 cm from the bottom of the plate?
What role does Dragendorff’s reagent play in TLC analysis after drying the plate?
What role does Dragendorff’s reagent play in TLC analysis after drying the plate?
How does failing to maintain a still chromatography tank affect TLC analysis results?
How does failing to maintain a still chromatography tank affect TLC analysis results?
What impact could a large spot size have on TLC analysis results?
What impact could a large spot size have on TLC analysis results?
Why is it crucial to ensure that all four reference standards and the nettle extract are applied to different crosses on the origin line during TLC analysis?
Why is it crucial to ensure that all four reference standards and the nettle extract are applied to different crosses on the origin line during TLC analysis?
