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TLC Plate Preparation and Analysis Methods

9 Questions

1 Views

TLC Plate Preparation and Analysis Methods

Created by

@EnterprisingRomanesque1303

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Questions and Answers

What is the purpose of gently blowing onto the applied spot until the solvent evaporates in TLC analysis?

To prevent contamination of the sample

To minimize spot spreading or enlargement (correct)

To speed up the process of solvent evaporation

To aid in the separation of components in the sample

Why is it essential to avoid scratching the surface of the TLC plate during sample application?

To speed up the analysis process

To prevent mixing of the samples

To ensure accurate calculation of Rf values

To avoid damaging the stationary phase (correct)

What is the purpose of marking the final position of the solvent front using a pencil after removing the TLC plate from the tank?

To help visualize the spots on the plate

To track the movement of the solvent front

To determine the Rf values of the samples

To guide in calculating the distance traveled by the solvent (correct)

Why is it recommended to use a designated capillary tube for each reference standard during sample application?

<p>To prevent cross-contamination</p> Signup and view all the answers

In TLC analysis, why should the mobile phase only come up to between 0.5 and 1 cm from the bottom of the plate?

<p>To prevent overdevelopment of the plate</p> Signup and view all the answers

What role does Dragendorff’s reagent play in TLC analysis after drying the plate?

<p>It visualizes spots on the plate for analysis</p> Signup and view all the answers

How does failing to maintain a still chromatography tank affect TLC analysis results?

<p>Results in incomplete separation of components</p> Signup and view all the answers

What impact could a large spot size have on TLC analysis results?

<p>Lead to underestimation of Rf values</p> Signup and view all the answers

Why is it crucial to ensure that all four reference standards and the nettle extract are applied to different crosses on the origin line during TLC analysis?

<p>To prevent contamination between standards and sample</p> Signup and view all the answers

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