Recent Lessons

Show all results for ""

Feature Overview

Ace your exams with our all-in-one platform for creating and sharing quizzes and tests.

Quizzes

Create quizzes and tests automatically from your content using AI.

Flashcards

Automatically turn your notes into digital flashcards.

Share, Export & Embed

Share with classmates or export to Excel and your learning management system.

Stats & Reporting

Auto-grading quizzes and tests with detailed stats and reports.

Mobile Apps

The smarter way to study – wherever you are.

Pricing

Search...

TLC Plate Preparation and Analysis Methods

9 Questions

1 Views

TLC Plate Preparation and Analysis Methods

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of gently blowing onto the applied spot until the solvent evaporates in TLC analysis?

To prevent contamination of the sample

To minimize spot spreading or enlargement (correct)

To speed up the process of solvent evaporation

To aid in the separation of components in the sample

Why is it essential to avoid scratching the surface of the TLC plate during sample application?

To speed up the analysis process

To prevent mixing of the samples

To ensure accurate calculation of Rf values

To avoid damaging the stationary phase (correct)

What is the purpose of marking the final position of the solvent front using a pencil after removing the TLC plate from the tank?

To help visualize the spots on the plate

To track the movement of the solvent front

To determine the Rf values of the samples

To guide in calculating the distance traveled by the solvent (correct)

Why is it recommended to use a designated capillary tube for each reference standard during sample application?

To prevent cross-contamination Signup and view all the answers

In TLC analysis, why should the mobile phase only come up to between 0.5 and 1 cm from the bottom of the plate?

To prevent overdevelopment of the plate Signup and view all the answers

What role does Dragendorff’s reagent play in TLC analysis after drying the plate?

It visualizes spots on the plate for analysis Signup and view all the answers

How does failing to maintain a still chromatography tank affect TLC analysis results?

Results in incomplete separation of components Signup and view all the answers

What impact could a large spot size have on TLC analysis results?

Lead to underestimation of Rf values Signup and view all the answers

Why is it crucial to ensure that all four reference standards and the nettle extract are applied to different crosses on the origin line during TLC analysis?

To prevent contamination between standards and sample Signup and view all the answers

TLC Plate Preparation and Analysis Methods

Choose a study mode

Podcast

Questions and Answers

What is the purpose of gently blowing onto the applied spot until the solvent evaporates in TLC analysis?

Why is it essential to avoid scratching the surface of the TLC plate during sample application?

What is the purpose of marking the final position of the solvent front using a pencil after removing the TLC plate from the tank?

Why is it recommended to use a designated capillary tube for each reference standard during sample application?

In TLC analysis, why should the mobile phase only come up to between 0.5 and 1 cm from the bottom of the plate?

What role does Dragendorff’s reagent play in TLC analysis after drying the plate?

How does failing to maintain a still chromatography tank affect TLC analysis results?

What impact could a large spot size have on TLC analysis results?

Why is it crucial to ensure that all four reference standards and the nettle extract are applied to different crosses on the origin line during TLC analysis?

More Like This

Chromatography TLC Plate Analysis

TLC Licensing Fees and Regulations Quiz

TLC Exam Questions Quiz & Flashcards for NYC

TLC Driver License Exam Quiz & Flashcards - NYC

Upgrade to continue