Chromatography Principles and Methods

Chromatography Principles and Methods

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@ComfyLearning2093

Questions and Answers

What is the term for the time between the sample injection and the maximum response for the solute's peak?

Retention Time

What is the term for the discrete section of a chromatographic column where there is equilibrium between the solute present in the stationary phase and the solute present in the mobile phase?

Theoretical Plates

Which detection method is based on the principle of refractive index and is a universal detector?

RI

What is the term for the separation of two components based on their interactions with the stationary and mobile phases in a liquid chromatographic process?

<p>Chromatographic Resolution</p> Signup and view all the answers

Which type of chromatography is based on the difference in capacity factors and distribution coefficients of the analytes using liquid stationary and mobile phases?

<p>Partition Chromatography</p> Signup and view all the answers

What is the type of chromatography where polar solvents elute later than non-polar lipophilic ones?

<p>Normal Phase Chromatography</p> Signup and view all the answers

What is the primary basis of separation in column chromatography?

<p>Difference in size and charges of the solute</p> Signup and view all the answers

What is the term used to describe the efficiency of a column in chromatography?

<p>Theoretical plates</p> Signup and view all the answers

What is the type of chromatography that uses a gas as the mobile phase?

<p>Gas Chromatography</p> Signup and view all the answers

What is the advantage of Thin-Layer Chromatography?

<p>Simple, rapid, and cheap</p> Signup and view all the answers

What is the effect of increasing the number of theoretical plates in a column?

<p>Chromatographic peaks become narrower</p> Signup and view all the answers

What is the material used as the stationary phase in Thin-Layer Chromatography?

<p>Silica or alumina</p> Signup and view all the answers

What is the primary principle of chromatography?

<p>Differential adsorption between a stationary phase and a mobile phase</p> Signup and view all the answers

What determines the rate at which a solute moves through a chromatography system?

<p>The solute's time spent in the stationary phase</p> Signup and view all the answers

What is the purpose of the stationary phase in chromatography?

<p>To facilitate the separation of the sample components</p> Signup and view all the answers

What is the result of a solute partitioning between the mobile phase and the stationary phase?

<p>The solute is distributed between the mobile phase and the stationary phase</p> Signup and view all the answers

What are the three main types of chromatography?

<p>Gas, liquid, and supercritical fluid chromatography</p> Signup and view all the answers

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