Chromatography Principles and Methods
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Questions and Answers

What is the term for the time between the sample injection and the maximum response for the solute's peak?

  • Column Efficiency
  • Chromatographic Resolution
  • Theoretical Plates
  • Retention Time (correct)
  • What is the term for the discrete section of a chromatographic column where there is equilibrium between the solute present in the stationary phase and the solute present in the mobile phase?

  • Retention Time
  • Column Efficiency
  • Chromatographic Resolution
  • Theoretical Plates (correct)
  • Which detection method is based on the principle of refractive index and is a universal detector?

  • RI (correct)
  • FD
  • UV
  • MS
  • What is the term for the separation of two components based on their interactions with the stationary and mobile phases in a liquid chromatographic process?

    <p>Chromatographic Resolution</p> Signup and view all the answers

    Which type of chromatography is based on the difference in capacity factors and distribution coefficients of the analytes using liquid stationary and mobile phases?

    <p>Partition Chromatography</p> Signup and view all the answers

    What is the type of chromatography where polar solvents elute later than non-polar lipophilic ones?

    <p>Normal Phase Chromatography</p> Signup and view all the answers

    What is the primary basis of separation in column chromatography?

    <p>Difference in size and charges of the solute</p> Signup and view all the answers

    What is the term used to describe the efficiency of a column in chromatography?

    <p>Theoretical plates</p> Signup and view all the answers

    What is the type of chromatography that uses a gas as the mobile phase?

    <p>Gas Chromatography</p> Signup and view all the answers

    What is the advantage of Thin-Layer Chromatography?

    <p>Simple, rapid, and cheap</p> Signup and view all the answers

    What is the effect of increasing the number of theoretical plates in a column?

    <p>Chromatographic peaks become narrower</p> Signup and view all the answers

    What is the material used as the stationary phase in Thin-Layer Chromatography?

    <p>Silica or alumina</p> Signup and view all the answers

    What is the primary principle of chromatography?

    <p>Differential adsorption between a stationary phase and a mobile phase</p> Signup and view all the answers

    What determines the rate at which a solute moves through a chromatography system?

    <p>The solute's time spent in the stationary phase</p> Signup and view all the answers

    What is the purpose of the stationary phase in chromatography?

    <p>To facilitate the separation of the sample components</p> Signup and view all the answers

    What is the result of a solute partitioning between the mobile phase and the stationary phase?

    <p>The solute is distributed between the mobile phase and the stationary phase</p> Signup and view all the answers

    What are the three main types of chromatography?

    <p>Gas, liquid, and supercritical fluid chromatography</p> Signup and view all the answers

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