Chromatography Exam Questions
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Questions and Answers

What is the purpose of the sample in chromatography?

  • To identify the stationary phase
  • To separate the components
  • To analyze the mobile phase
  • To introduce the mixture into the system (correct)
  • What role does the solvent play in chromatography?

  • It acts as the stationary phase
  • It measures the components
  • It analyses the sample
  • It creates the mobile phase (correct)
  • Which chromatography technique is known for its ability to separate and purify proteins?

  • Gas chromatography
  • Ion-exchange chromatography
  • Affinity chromatography (correct)
  • Reverse-phase chromatography
  • What component of a mixture moves faster in chromatography if it is less attracted to the stationary phase?

    <p>The lighter component</p> Signup and view all the answers

    What is the purpose of a mobile phase in high performance liquid chromatography?

    <p>To carry the sample through the column</p> Signup and view all the answers

    Study Notes

    Chromatography Basics

    • Sample introduction is the primary purpose of the sample in chromatography

    Chromatography Process

    • Solvent plays a crucial role in creating the mobile phase in chromatography

    Protein Separation and Purification

    • Affinity chromatography technique is particularly effective in separating and purifying proteins

    Component Migration

    • Lighter components of a mixture migrate faster in chromatography due to lesser attraction to the stationary phase

    High-Performance Liquid Chromatography (HPLC)

    • Mobile phase in HPLC carries the sample through the column, facilitating the separation process

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    Description

    Quiz on chromatography techniques, including the purpose of samples, solvents, and affinity chromatography. Test your knowledge of chromatography principles and concepts.

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