Podcast
Questions and Answers
What is the purpose of the sample in chromatography?
What is the purpose of the sample in chromatography?
- To identify the stationary phase
- To separate the components
- To analyze the mobile phase
- To introduce the mixture into the system (correct)
What role does the solvent play in chromatography?
What role does the solvent play in chromatography?
- It acts as the stationary phase
- It measures the components
- It analyses the sample
- It creates the mobile phase (correct)
Which chromatography technique is known for its ability to separate and purify proteins?
Which chromatography technique is known for its ability to separate and purify proteins?
- Gas chromatography
- Ion-exchange chromatography
- Affinity chromatography (correct)
- Reverse-phase chromatography
What component of a mixture moves faster in chromatography if it is less attracted to the stationary phase?
What component of a mixture moves faster in chromatography if it is less attracted to the stationary phase?
What is the purpose of a mobile phase in high performance liquid chromatography?
What is the purpose of a mobile phase in high performance liquid chromatography?
Flashcards
Purpose of the sample
Purpose of the sample
To introduce the mixture into the chromatography system for separation and analysis.
Solvent's role in chromatography
Solvent's role in chromatography
It acts as the mobile phase, carrying the sample components through the stationary phase.
Affinity chromatography
Affinity chromatography
A separation technique based on highly specific interactions between a protein and a binding partner immobilized on a solid support.
Faster movement in chromatography
Faster movement in chromatography
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Mobile phase purpose in HPLC
Mobile phase purpose in HPLC
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Study Notes
Chromatography Basics
- Sample introduction is the primary purpose of the sample in chromatography
Chromatography Process
- Solvent plays a crucial role in creating the mobile phase in chromatography
Protein Separation and Purification
- Affinity chromatography technique is particularly effective in separating and purifying proteins
Component Migration
- Lighter components of a mixture migrate faster in chromatography due to lesser attraction to the stationary phase
High-Performance Liquid Chromatography (HPLC)
- Mobile phase in HPLC carries the sample through the column, facilitating the separation process
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