Chromatography: Principles and Applications Quiz
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Questions and Answers

What is the stationary phase in chromatography?

  • A gas or a liquid which flows continuously around the stationary phase
  • The individual components of a mixture
  • The component of the mixture being separated
  • A solid or liquid supported on a solid (correct)

    • What is the mobile phase in chromatography?

  • The component of the mixture being separated
  • The individual components of a mixture
  • A gas or a liquid which flows continuously around the stationary phase (correct)
  • A solid or liquid supported on a solid

    • What is the purpose of chromatography?

  • To identify a single component in a mixture
  • To mix different chemical substances together
  • To analyze solid substances only
  • To separate a mixture of chemical substances into its individual components (correct)

    • What is the main function of chromatographic techniques?

    <p>Enable the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis</p> Signup and view all the answers

    What does the mobile phase do in chromatography?

    <p>Carries the components with it as it flows continuously around the stationary phase</p> Signup and view all the answers

    What is the eluent in liquid chromatography?

    <p>The liquid solvent</p> Signup and view all the answers

    What is the purpose of the mobile phase in chromatography?

    <p>To separate and analyze the mixture's components</p> Signup and view all the answers

    What determines the separation of molecules from each other in chromatography?

    <p>Molecular weight differences</p> Signup and view all the answers

    What characteristic leads some components of a mixture to stay longer in the stationary phase during chromatography?

    <p>Polarity</p> Signup and view all the answers

    In which type of chromatography is paper used as the stationary phase?

    <p>Thin layer chromatography</p> Signup and view all the answers

    What is the primary purpose of chromatography?

    <p>To identify components of a mixture</p> Signup and view all the answers

    In chromatography, what is the role of the stationary phase?

    <p>To interact with the components of the mixture</p> Signup and view all the answers

    Which type of chromatography uses a solid stationary phase?

    <p>Thin-layer chromatography</p> Signup and view all the answers

    What is the main function of the mobile phase in chromatography?

    <p>To interact with the stationary phase</p> Signup and view all the answers

    How does chromatography enable qualitative and quantitative analysis?

    <p>By identifying and purifying the components of a mixture</p> Signup and view all the answers

    What is the substance that stays fixed inside the column in chromatography?

    <p>Stationary phase</p> Signup and view all the answers

    What is the carrier portion of the mobile phase in chromatography?

    <p>Eluent</p> Signup and view all the answers

    In chromatography, what specifically includes both the analytes and solutes passing through the column?

    <p>Eluate/elute</p> Signup and view all the answers

    What term is used for the distance traveled by the sample divided by the distance traveled by the solvent in chromatography?

    <p>Retention factor (Rf)</p> Signup and view all the answers

    What determines the separation of molecules from each other in chromatography?

    <p>The type of interaction between stationary phase, mobile phase, and substances contained in the mixture</p> Signup and view all the answers

    Study Notes

    Chromatography Basics

    • Chromatography is a laboratory technique used to separate, identify, and quantify the components of a mixture.

    Phases in Chromatography

    • The mobile phase is a substance that moves through the chromatography system, carrying the analytes (substances being analyzed) with it.
    • The mobile phase can be a liquid or a gas, depending on the type of chromatography.
    • The stationary phase is a substance that remains fixed in place, interacting with the analytes as they pass through.

    Function of Phases

    • The mobile phase transports the analytes through the system, allowing them to interact with the stationary phase.
    • The stationary phase separates the analytes based on their properties, such as adsorption, partition, or affinity.

    Purpose of Chromatography

    • The primary purpose of chromatography is to separate, identify, and quantify the components of a mixture.

    Types of Chromatography

    • Paper chromatography uses paper as the stationary phase.
    • Solid-phase chromatography uses a solid stationary phase.

    Chromatography Terms

    • The eluent is the solvent used in liquid chromatography to carry the analytes through the system.
    • The carrier portion of the mobile phase is the solvent that carries the analytes.
    • The term "retention factor" is used to describe the distance traveled by the sample divided by the distance traveled by the solvent.

    Separation Mechanism

    • Separation of molecules is determined by their interactions with the stationary phase, such as adsorption, partition, or affinity.
    • The characteristic that leads some components to stay longer in the stationary phase is their higher affinity for the stationary phase.

    Analysis

    • Chromatography enables qualitative and quantitative analysis by separating the analytes and allowing their detection and measurement.

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    Description

    Test your knowledge of chromatography principles, classifications, and applications with this quiz. Learn to separate various mixtures using chromatography and explore its analytical techniques.

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