Chromatography Quiz: Principles and Applications

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Questions and Answers

What is the stationary phase in chromatography?

A gas or a liquid which flows continuously around the stationary phase

The individual components of a mixture

The component of the mixture being separated

A solid or liquid supported on a solid (correct)

What is the mobile phase in chromatography?

The component of the mixture being separated

The individual components of a mixture

A gas or a liquid which flows continuously around the stationary phase (correct)

A solid or liquid supported on a solid

What is the purpose of chromatography?

To identify a single component in a mixture

To mix different chemical substances together

To analyze solid substances only

To separate a mixture of chemical substances into its individual components (correct)

What is the main function of chromatographic techniques?

Enable the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis Signup and view all the answers

What does the mobile phase do in chromatography?

Carries the components with it as it flows continuously around the stationary phase Signup and view all the answers

What is the eluent in liquid chromatography?

The liquid solvent Signup and view all the answers

What is the purpose of the mobile phase in chromatography?

To separate and analyze the mixture's components Signup and view all the answers

What determines the separation of molecules from each other in chromatography?

Molecular weight differences Signup and view all the answers

What characteristic leads some components of a mixture to stay longer in the stationary phase during chromatography?

Polarity Signup and view all the answers

In which type of chromatography is paper used as the stationary phase?

Thin layer chromatography Signup and view all the answers

What is the primary purpose of chromatography?

To identify components of a mixture Signup and view all the answers

In chromatography, what is the role of the stationary phase?

To interact with the components of the mixture Signup and view all the answers

Which type of chromatography uses a solid stationary phase?

Thin-layer chromatography Signup and view all the answers

What is the main function of the mobile phase in chromatography?

To interact with the stationary phase Signup and view all the answers

How does chromatography enable qualitative and quantitative analysis?

By identifying and purifying the components of a mixture Signup and view all the answers

What is the substance that stays fixed inside the column in chromatography?

Stationary phase Signup and view all the answers

What is the carrier portion of the mobile phase in chromatography?

Eluent Signup and view all the answers

In chromatography, what specifically includes both the analytes and solutes passing through the column?

Eluate/elute Signup and view all the answers

What term is used for the distance traveled by the sample divided by the distance traveled by the solvent in chromatography?

Retention factor (Rf) Signup and view all the answers

What determines the separation of molecules from each other in chromatography?

The type of interaction between stationary phase, mobile phase, and substances contained in the mixture Signup and view all the answers

Study Notes

Chromatography Basics

Chromatography is a laboratory technique used to separate, identify, and quantify the components of a mixture.

Phases in Chromatography

The mobile phase is a substance that moves through the chromatography system, carrying the analytes (substances being analyzed) with it.
The mobile phase can be a liquid or a gas, depending on the type of chromatography.
The stationary phase is a substance that remains fixed in place, interacting with the analytes as they pass through.

Function of Phases

The mobile phase transports the analytes through the system, allowing them to interact with the stationary phase.
The stationary phase separates the analytes based on their properties, such as adsorption, partition, or affinity.

Purpose of Chromatography

The primary purpose of chromatography is to separate, identify, and quantify the components of a mixture.

Types of Chromatography

Paper chromatography uses paper as the stationary phase.
Solid-phase chromatography uses a solid stationary phase.

Chromatography Terms

The eluent is the solvent used in liquid chromatography to carry the analytes through the system.
The carrier portion of the mobile phase is the solvent that carries the analytes.
The term "retention factor" is used to describe the distance traveled by the sample divided by the distance traveled by the solvent.

Separation Mechanism

Separation of molecules is determined by their interactions with the stationary phase, such as adsorption, partition, or affinity.
The characteristic that leads some components to stay longer in the stationary phase is their higher affinity for the stationary phase.

Analysis

Chromatography enables qualitative and quantitative analysis by separating the analytes and allowing their detection and measurement.

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Description

Test your knowledge of chromatography principles, classifications, and applications with this quiz. Learn to separate various mixtures using chromatography and explore its analytical techniques.