12 Questions
Match the following forces that drive the separation in chromatography with their description:
Relative affinities of components of the mixture toward mobile phase and stationary phase = Principle of chromatography Interaction of the sample with the mobile phase and stationary phase = Principle of chromatography Change of physical properties of mixture between mobile and stationary layers = Principle of chromatography Distribution of analyte between the mobile phase and stationary phase = Principle of chromatography
Match the types of chromatography with their characteristics:
Column chromatography = Separation technique using a column filled with stationary phase material Gas chromatography = Separation technique based on vaporization and volatilization of components Thin-layer chromatography = Separation technique using a thin layer of adsorbent material on a glass or plastic plate Liquid chromatography = Separation technique using liquid as the mobile phase
Match the following terms used in chromatography with their meanings:
Chromatography = Color writing method Stationary phase = Phase that does not move in the separation process Mobile phase = Phase that travels over or through the stationary phase Supercritical fluids = Type of mobile phase in chromatography
Match the following chromatography techniques with their descriptions:
Column Chromatography = Involves filling a column with stationary phase and using solvent as mobile phase Thin Layer Chromatography (TLC) = Uses a thin stationary phase supported by an inert backing to separate components of a mixture Paper Chromatography = Separates components based on their affinity to paper as stationary phase and solvent as mobile phase Gas Chromatography = Utilizes gas as the mobile phase to separate compounds based on their interaction with the stationary phase
Match the following terms with their definitions in chromatography:
Stationary Phase = The immobile phase in chromatography that interacts with the mobile phase and separates components Mobile Phase = The moving phase in chromatography that carries the sample through the stationary phase for separation Chromatography = A technique used to separate components of a mixture based on their affinities to stationary and mobile phases Principle of Chromatography = Compounds have different affinities for the stationary and mobile phases, affecting their migration speed
Match the following actions with the appropriate steps in column chromatography:
Filling column with silica powder = Creating the stationary phase in column chromatography Pouring sample mixture into the top of the column = Introducing the mixture to be separated in column chromatography Opening tap at the bottom while pouring solvent = Allowing the solvent to carry the sample through silica powder in column chromatography Saturating silica powder with solvent = Preparing the mobile phase in column chromatography
Match the forces involved in chromatography with their description:
Adsorption = Attraction of analytes to the stationary phase Partitioning = Distribution of analytes between mobile and stationary phases Ion exchange = Separation based on charge interactions Size exclusion = Separation based on molecular size
Match the types of chromatography with their primary principle:
Column Chromatography = Separation based on adsorption and partitioning Ion Exchange Chromatography = Separation based on charge interactions Size Exclusion Chromatography = Separation based on molecular size Affinity Chromatography = Separation based on specific binding interactions
Match the components of chromatography with their roles:
Stationary Phase = Provides surface for analyte interaction Mobile Phase = Aids in elution of separated components Developing Agent = Assists in forming bands in the sample Eluting Agent = Removes separated components from the column
Match the characteristics of a good stationary phase with their descriptions:
Uniform shape and size = Particles should be consistent in dimensions Mechanical stability = Solid material should withstand pressure during chromatography Chemically inert = Should not react with solvents or compounds being separated Allow free flow of mobile phase = Should not impede the movement of solvents and analytes
Match the solvents with their function in chromatography:
Ethanol = Used as a mobile phase solvent Acetone = Aids in eluting separated components Water = Can be introduced in the column as a solvent-sample mixture Acetic Acid = Helps in separation of components by forming bands
Match the meaning of chromatography terms with their definitions:
Adsorption Chromatography = Separation based on surface interactions Partitioning Chromatography = Separation based on distribution between phases Ion Exchange Chromatography = Separation based on charge interactions Affinity Chromatography = Separation based on specific binding interactions
Learn about the chromatographic technique, an analytical method used for separating and identifying components in a mixture. Understand the principles behind the separation process involving stationary and mobile phases.
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