Chromatographic Technique Basics

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Match the following forces that drive the separation in chromatography with their description:

Relative affinities of components of the mixture toward mobile phase and stationary phase = Principle of chromatography Interaction of the sample with the mobile phase and stationary phase = Principle of chromatography Change of physical properties of mixture between mobile and stationary layers = Principle of chromatography Distribution of analyte between the mobile phase and stationary phase = Principle of chromatography

Match the types of chromatography with their characteristics:

Column chromatography = Separation technique using a column filled with stationary phase material Gas chromatography = Separation technique based on vaporization and volatilization of components Thin-layer chromatography = Separation technique using a thin layer of adsorbent material on a glass or plastic plate Liquid chromatography = Separation technique using liquid as the mobile phase

Match the following terms used in chromatography with their meanings:

Chromatography = Color writing method Stationary phase = Phase that does not move in the separation process Mobile phase = Phase that travels over or through the stationary phase Supercritical fluids = Type of mobile phase in chromatography

Match the following chromatography techniques with their descriptions:

Column Chromatography = Involves filling a column with stationary phase and using solvent as mobile phase Thin Layer Chromatography (TLC) = Uses a thin stationary phase supported by an inert backing to separate components of a mixture Paper Chromatography = Separates components based on their affinity to paper as stationary phase and solvent as mobile phase Gas Chromatography = Utilizes gas as the mobile phase to separate compounds based on their interaction with the stationary phase Signup and view all the answers

Match the following terms with their definitions in chromatography:

Stationary Phase = The immobile phase in chromatography that interacts with the mobile phase and separates components Mobile Phase = The moving phase in chromatography that carries the sample through the stationary phase for separation Chromatography = A technique used to separate components of a mixture based on their affinities to stationary and mobile phases Principle of Chromatography = Compounds have different affinities for the stationary and mobile phases, affecting their migration speed Signup and view all the answers

Match the following actions with the appropriate steps in column chromatography:

Filling column with silica powder = Creating the stationary phase in column chromatography Pouring sample mixture into the top of the column = Introducing the mixture to be separated in column chromatography Opening tap at the bottom while pouring solvent = Allowing the solvent to carry the sample through silica powder in column chromatography Saturating silica powder with solvent = Preparing the mobile phase in column chromatography Signup and view all the answers

Match the forces involved in chromatography with their description:

Adsorption = Attraction of analytes to the stationary phase Partitioning = Distribution of analytes between mobile and stationary phases Ion exchange = Separation based on charge interactions Size exclusion = Separation based on molecular size Signup and view all the answers

Match the types of chromatography with their primary principle:

Column Chromatography = Separation based on adsorption and partitioning Ion Exchange Chromatography = Separation based on charge interactions Size Exclusion Chromatography = Separation based on molecular size Affinity Chromatography = Separation based on specific binding interactions Signup and view all the answers

Match the components of chromatography with their roles:

Stationary Phase = Provides surface for analyte interaction Mobile Phase = Aids in elution of separated components Developing Agent = Assists in forming bands in the sample Eluting Agent = Removes separated components from the column Signup and view all the answers

Match the characteristics of a good stationary phase with their descriptions:

Uniform shape and size = Particles should be consistent in dimensions Mechanical stability = Solid material should withstand pressure during chromatography Chemically inert = Should not react with solvents or compounds being separated Allow free flow of mobile phase = Should not impede the movement of solvents and analytes Signup and view all the answers

Match the solvents with their function in chromatography:

Ethanol = Used as a mobile phase solvent Acetone = Aids in eluting separated components Water = Can be introduced in the column as a solvent-sample mixture Acetic Acid = Helps in separation of components by forming bands Signup and view all the answers

Match the meaning of chromatography terms with their definitions:

Adsorption Chromatography = Separation based on surface interactions Partitioning Chromatography = Separation based on distribution between phases Ion Exchange Chromatography = Separation based on charge interactions Affinity Chromatography = Separation based on specific binding interactions Signup and view all the answers