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Separating Components with Thin Layer Chromatography

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Separating Components with Thin Layer Chromatography

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Questions and Answers

What is the main purpose of Thin Layer Chromatography (TLC)?

To monitor the progress of a reaction (correct)

To speed up the reaction time

To increase the cost of separation

To combine components of a mixture

Why is TLC considered an analytical tool widely used?

Because it is expensive

Due to its complexity

For its high sensitivity and speed of separation (correct)

For its large scale purification capabilities

What is the function of the TLC chamber in Thin Layer Chromatography (TLC)?

To apply the stationary phase

To maintain a uniform environment for spot development (correct)

To speed up the migration of compounds

To prevent the separation of components

Why is it important for TLC plates to have a thin layer of stationary phase?

To ensure uniform thickness for separation (C) Signup and view all the answers

What principle does TLC operate on?

Compound's affinities for mobile and stationary phases (A) Signup and view all the answers

What is the goal of TLC in terms of separated spots?

To obtain well-defined, well-separated spots (C) Signup and view all the answers

What is the purpose of using a particulate-free and high purity mobile phase in Thin Layer Chromatography (TLC)?

To improve the development of TLC spots (B) Signup and view all the answers

What is the main function of the moistened filter paper placed in the TLC chamber during the TLC procedure?

To create a humidity-controlled environment (C) Signup and view all the answers

In Thin Layer Chromatography (TLC), why is it important to ensure that the sample spots are well above the level of the mobile phase?

To allow for better sample separation (A) Signup and view all the answers

What is the significance of the Retention Factor (Rf) in Thin Layer Chromatography (TLC)?

It represents a constant value for each compound under constant conditions (C) Signup and view all the answers

How does a TLC plate coated with silica particles affect the movement of polar and non-polar molecules?

It retains non-polar molecules due to hydrogen bonding interactions with silica (B) Signup and view all the answers

What can cause fluctuations in Retention Factor (Rf) values from one TLC experiment to another?

Changes in the thickness of the adsorbent layer on the TLC plate (B) Signup and view all the answers