Podcast
Questions and Answers
What is the main purpose of Thin Layer Chromatography (TLC)?
What is the main purpose of Thin Layer Chromatography (TLC)?
- To monitor the progress of a reaction (correct)
- To speed up the reaction time
- To increase the cost of separation
- To combine components of a mixture
Why is TLC considered an analytical tool widely used?
Why is TLC considered an analytical tool widely used?
- Because it is expensive
- Due to its complexity
- For its high sensitivity and speed of separation (correct)
- For its large scale purification capabilities
What is the function of the TLC chamber in Thin Layer Chromatography (TLC)?
What is the function of the TLC chamber in Thin Layer Chromatography (TLC)?
- To apply the stationary phase
- To maintain a uniform environment for spot development (correct)
- To speed up the migration of compounds
- To prevent the separation of components
Why is it important for TLC plates to have a thin layer of stationary phase?
Why is it important for TLC plates to have a thin layer of stationary phase?
What principle does TLC operate on?
What principle does TLC operate on?
What is the goal of TLC in terms of separated spots?
What is the goal of TLC in terms of separated spots?
What is the purpose of using a particulate-free and high purity mobile phase in Thin Layer Chromatography (TLC)?
What is the purpose of using a particulate-free and high purity mobile phase in Thin Layer Chromatography (TLC)?
What is the main function of the moistened filter paper placed in the TLC chamber during the TLC procedure?
What is the main function of the moistened filter paper placed in the TLC chamber during the TLC procedure?
In Thin Layer Chromatography (TLC), why is it important to ensure that the sample spots are well above the level of the mobile phase?
In Thin Layer Chromatography (TLC), why is it important to ensure that the sample spots are well above the level of the mobile phase?
What is the significance of the Retention Factor (Rf) in Thin Layer Chromatography (TLC)?
What is the significance of the Retention Factor (Rf) in Thin Layer Chromatography (TLC)?
How does a TLC plate coated with silica particles affect the movement of polar and non-polar molecules?
How does a TLC plate coated with silica particles affect the movement of polar and non-polar molecules?
What can cause fluctuations in Retention Factor (Rf) values from one TLC experiment to another?
What can cause fluctuations in Retention Factor (Rf) values from one TLC experiment to another?