Separation of Mixture using Thin Layer Chromatography

In Thin Layer Chromatography (TLC), the mobile phase is supported by an inert backing.

TLC may be performed on the analytical scale to purify large amounts of a compound.

The goal of TLC is to obtain blurred, poorly separated spots.

TLC plates have a thick layer of stationary phase applied on their whole surface layer.

The TLC chamber is used to prevent the evaporation of solvents during the development of the TLC plate.

In Thin Layer Chromatography (TLC), the compound with different affinities for the mobile and stationary phases will migrate at the same speed.

The mobile phase in Thin Layer Chromatography (TLC) should be particulate-free for proper development of spots.

The stationary phase in TLC is applied uniformly on the plate, then allowed to dry and stabilize before applying the sample spots.

A thin mark at the top of the plate is made with a pencil for applying the sample spots in TLC.

The TLC plate should be immersed in the solvent during the development process to ensure accurate results.

Edge effect in TLC can be avoided by placing a moistened filter paper in the mobile phase on the outer wall of the chamber.

The behavior of a compound on a TLC plate is usually described in terms of its Relative Factor (RF) value.

Rf value is a constant value for a compound, regardless of the chromatography conditions.

In TLC, the spot containing a nonpolar molecule rises quickly with the solvent front.

Phenol, being a polar molecule, rises quickly with the solvent front in TLC.

The solvent system used in TLC has no effect on the separation of compounds on the TLC plate.

Changing the temperature during a TLC experiment will not alter the Rf values of compounds.

The thickness of the adsorbent layer in TLC has no impact on the separation of compounds.