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Questions and Answers
What is the primary purpose of conducting lab tests?
What is the primary purpose of conducting lab tests?
To screen for disease, evaluate drug therapy, confirm a diagnosis, or rule out a clinical problem.
How do normal ranges differ from reference ranges in lab tests?
How do normal ranges differ from reference ranges in lab tests?
Normal ranges are based on healthy individuals, while reference ranges vary by age and gender.
What condition might a lower albumin level indicate?
What condition might a lower albumin level indicate?
Malnutrition or liver disease.
Why is it important to interpret lab results in context?
Why is it important to interpret lab results in context?
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What are critical values in laboratory diagnostics?
What are critical values in laboratory diagnostics?
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What is the role of albumin in the body?
What is the role of albumin in the body?
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Identify one interfering factor that may affect albumin test results.
Identify one interfering factor that may affect albumin test results.
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What lab tests are primarily used to measure chemical components in the body?
What lab tests are primarily used to measure chemical components in the body?
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What enzyme is primarily responsible for converting ammonia to urea in the body?
What enzyme is primarily responsible for converting ammonia to urea in the body?
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What effect can smoking have on ammonia blood levels?
What effect can smoking have on ammonia blood levels?
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What is a common critical threshold for total bilirubin in adults?
What is a common critical threshold for total bilirubin in adults?
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What is the range for normal total bilirubin levels in adults?
What is the range for normal total bilirubin levels in adults?
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Which syndrome is associated with defective bilirubin conjugation and may lead to jaundice?
Which syndrome is associated with defective bilirubin conjugation and may lead to jaundice?
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How can strenuous exercise affect ammonia test results?
How can strenuous exercise affect ammonia test results?
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What is the role of the kidneys concerning urea and ammonia?
What is the role of the kidneys concerning urea and ammonia?
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What pathological condition can cause elevated levels of conjugated bilirubin?
What pathological condition can cause elevated levels of conjugated bilirubin?
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What dietary factors can interfere with ammonia test results?
What dietary factors can interfere with ammonia test results?
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How does newborn hemolysis relate to bilirubin production?
How does newborn hemolysis relate to bilirubin production?
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What condition can lead to decreased hepatic clearance of bilirubin?
What condition can lead to decreased hepatic clearance of bilirubin?
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What is one effect of broad-spectrum antibiotics on ammonia levels?
What is one effect of broad-spectrum antibiotics on ammonia levels?
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In cases of jaundice, what are the potential anatomical sites of pathology for elevated bilirubin?
In cases of jaundice, what are the potential anatomical sites of pathology for elevated bilirubin?
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What is an inference drawn from elevated indirect bilirubin levels?
What is an inference drawn from elevated indirect bilirubin levels?
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What are some drugs associated with isolated elevated bilirubin levels?
What are some drugs associated with isolated elevated bilirubin levels?
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Which medical conditions can lead to conjugated hyperbilirubinemia?
Which medical conditions can lead to conjugated hyperbilirubinemia?
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Describe the solubility characteristics of unconjugated bilirubin.
Describe the solubility characteristics of unconjugated bilirubin.
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What is the primary form of conjugated bilirubin found in the body?
What is the primary form of conjugated bilirubin found in the body?
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How does bilirubin enter the small intestine?
How does bilirubin enter the small intestine?
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What causes the characteristic colors of urine and stool related to bilirubin?
What causes the characteristic colors of urine and stool related to bilirubin?
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At what bilirubin level is jaundice typically observed?
At what bilirubin level is jaundice typically observed?
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What is the significance of dark urine in hyperbilirubinemia?
What is the significance of dark urine in hyperbilirubinemia?
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Name a symptom that can be seen in chronic liver disease.
Name a symptom that can be seen in chronic liver disease.
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What test type is bilirubin testing usually performed alongside?
What test type is bilirubin testing usually performed alongside?
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What is the primary cause of Crigler-Najjar syndrome, and how does it differ between type I and type II?
What is the primary cause of Crigler-Najjar syndrome, and how does it differ between type I and type II?
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How does Gilbert syndrome differ from Crigler-Najjar syndrome despite both involving decreased UGT activity?
How does Gilbert syndrome differ from Crigler-Najjar syndrome despite both involving decreased UGT activity?
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What distinguishes primary sclerosing cholangitis (PSC) from other forms of biliary obstruction?
What distinguishes primary sclerosing cholangitis (PSC) from other forms of biliary obstruction?
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What is the underlying genetic defect in Dubin-Johnson syndrome (DJS) and how does it present biochemically?
What is the underlying genetic defect in Dubin-Johnson syndrome (DJS) and how does it present biochemically?
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What are the key clinical features of primary biliary cirrhosis?
What are the key clinical features of primary biliary cirrhosis?
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In patients with Wilson disease, what is the typical biochemical finding, and what is the treatment?
In patients with Wilson disease, what is the typical biochemical finding, and what is the treatment?
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How does Rotor syndrome compare to Dubin-Johnson syndrome in terms of genetic defect and clinical presentation?
How does Rotor syndrome compare to Dubin-Johnson syndrome in terms of genetic defect and clinical presentation?
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What factors contribute to the pathogenesis of TPN-induced cholestasis?
What factors contribute to the pathogenesis of TPN-induced cholestasis?
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What is AIDS cholangiopathy, and what is its primary cause?
What is AIDS cholangiopathy, and what is its primary cause?
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What defines benign recurrent intrahepatic cholestasis (BRIC) and how does it impact liver health?
What defines benign recurrent intrahepatic cholestasis (BRIC) and how does it impact liver health?
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What distinguishes conjugated hyperbilirubinemia from unconjugated hyperbilirubinemia in clinical terms?
What distinguishes conjugated hyperbilirubinemia from unconjugated hyperbilirubinemia in clinical terms?
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Identify the major categories contributing to unconjugated hyperbilirubinemia.
Identify the major categories contributing to unconjugated hyperbilirubinemia.
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What are the common forms of biliary obstruction and their implications?
What are the common forms of biliary obstruction and their implications?
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What is the main purpose of measuring albumin levels in laboratory tests?
What is the main purpose of measuring albumin levels in laboratory tests?
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Which of the following factors may interfere with albumin test results?
Which of the following factors may interfere with albumin test results?
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What condition can lead to an abnormally high level of albumin in serum?
What condition can lead to an abnormally high level of albumin in serum?
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What is indicated by a lower than normal albumin level?
What is indicated by a lower than normal albumin level?
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How are normal ranges for laboratory tests typically established?
How are normal ranges for laboratory tests typically established?
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What is the consequence of failing to act on critical lab values?
What is the consequence of failing to act on critical lab values?
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In what way can reference ranges vary?
In what way can reference ranges vary?
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What is an important consideration for follow-up assessments post-lab tests?
What is an important consideration for follow-up assessments post-lab tests?
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Which of the following drugs is known to cause isolated elevated bilirubin levels?
Which of the following drugs is known to cause isolated elevated bilirubin levels?
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What is the primary process through which bilirubin is conjugated in the liver?
What is the primary process through which bilirubin is conjugated in the liver?
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What is the expected appearance of feces in a condition with absent urobilinogens?
What is the expected appearance of feces in a condition with absent urobilinogens?
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Which of the following symptoms is most commonly associated with jaundice due to hyperbilirubinemia?
Which of the following symptoms is most commonly associated with jaundice due to hyperbilirubinemia?
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What is the primary cause of unconjugated hyperbilirubinemia?
What is the primary cause of unconjugated hyperbilirubinemia?
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What characteristic feature differentiates dark urine in conjugated hyperbilirubinemia from that in unconjugated hyperbilirubinemia?
What characteristic feature differentiates dark urine in conjugated hyperbilirubinemia from that in unconjugated hyperbilirubinemia?
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Which condition is NOT a known cause of conjugated hyperbilirubinemia?
Which condition is NOT a known cause of conjugated hyperbilirubinemia?
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What laboratory tests are typically conducted alongside bilirubin testing?
What laboratory tests are typically conducted alongside bilirubin testing?
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What process allows the reabsorption of urobilinogen back to the liver after intestinal bacteria convert bilirubin?
What process allows the reabsorption of urobilinogen back to the liver after intestinal bacteria convert bilirubin?
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What is the main indication for measuring ammonia levels in the blood?
What is the main indication for measuring ammonia levels in the blood?
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Which of the following factors can contribute to elevated bilirubin levels?
Which of the following factors can contribute to elevated bilirubin levels?
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What range is considered critical for total bilirubin levels in adults?
What range is considered critical for total bilirubin levels in adults?
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How does smoking affect ammonia levels in the blood?
How does smoking affect ammonia levels in the blood?
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Which of the following pathologies is most likely to cause elevated unconjugated bilirubin?
Which of the following pathologies is most likely to cause elevated unconjugated bilirubin?
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What factor may lead to decreased hepatic clearance of bilirubin?
What factor may lead to decreased hepatic clearance of bilirubin?
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What is a common cause of conjugated hyperbilirubinemia?
What is a common cause of conjugated hyperbilirubinemia?
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Which type of bilirubin is primarily associated with liver dysfunction?
Which type of bilirubin is primarily associated with liver dysfunction?
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What is the primary metabolic process associated with ammonia in the body?
What is the primary metabolic process associated with ammonia in the body?
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What role does the liver play in the metabolism of bilirubin?
What role does the liver play in the metabolism of bilirubin?
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What is the potential impact of using broad-spectrum antibiotics on bilirubin levels?
What is the potential impact of using broad-spectrum antibiotics on bilirubin levels?
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What condition may indicate the presence of elevated direct bilirubin levels?
What condition may indicate the presence of elevated direct bilirubin levels?
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What is a prevalent condition associated with unconjugated hyperbilirubinemia in newborns?
What is a prevalent condition associated with unconjugated hyperbilirubinemia in newborns?
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What laboratory test is often performed alongside bilirubin testing for a comprehensive evaluation?
What laboratory test is often performed alongside bilirubin testing for a comprehensive evaluation?
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What is the main difference between type I and type II Crigler-Najjar syndrome?
What is the main difference between type I and type II Crigler-Najjar syndrome?
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Which condition is characterized by a mutation in the promoter region affecting UGT activity?
Which condition is characterized by a mutation in the promoter region affecting UGT activity?
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What is the primary treatment for type I Crigler-Najjar syndrome?
What is the primary treatment for type I Crigler-Najjar syndrome?
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Which factor is associated with conjugated hyperbilirubinemia due to liver infiltration?
Which factor is associated with conjugated hyperbilirubinemia due to liver infiltration?
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What is the characteristic finding in Dubin-Johnson syndrome?
What is the characteristic finding in Dubin-Johnson syndrome?
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What usually causes total parenteral nutrition (TPN)–induced cholestasis?
What usually causes total parenteral nutrition (TPN)–induced cholestasis?
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What is a key clinical feature of primary biliary cirrhosis?
What is a key clinical feature of primary biliary cirrhosis?
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Which condition is associated with biliary obstruction secondary to infection?
Which condition is associated with biliary obstruction secondary to infection?
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Which of the following conditions does NOT typically require medical treatment?
Which of the following conditions does NOT typically require medical treatment?
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What is a major risk factor for cholangiocarcinoma in patients with PSC?
What is a major risk factor for cholangiocarcinoma in patients with PSC?
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What type of bilirubin is elevated in chronic hepatitis infections?
What type of bilirubin is elevated in chronic hepatitis infections?
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Which syndrome is characterized by recurrent jaundice and pruritus but resolves without liver damage?
Which syndrome is characterized by recurrent jaundice and pruritus but resolves without liver damage?
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What role does phenobarbital play in the management of hyperbilirubinemia?
What role does phenobarbital play in the management of hyperbilirubinemia?
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Study Notes
Chemistry Tests
-
Albumin (AL)
- Normal Range: 35-50 g/L
- Used to diagnose and monitor liver diseases, impaired nutrition, chronic edema, and cancer
- Albumin is a protein made in the liver and maintains colloidal osmotic pressure
- Lower levels can indicate: malnutrition, liver disease, ascites, inflammatory disease, autoimmune disorder, GI malabsorption syndromes, or pregnancy
- Higher levels can indicate: severe dehydration
-
Ammonia (AMM, NH3)
- Normal Range: 6-47 mcmol/L
- Measures the amount of ammonia in the blood
- Ammonia is created by the breakdown of protein; the liver converts ammonia to urea
- Used to diagnose severe liver diseases
- Lower levels can indicate: Essential or malignant hypertension
- Higher levels can indicate: Liver disease (cirrhosis or hepatitis), Reye syndrome, heart failure, kidney failure, severe bleeding from the stomach or intestines
-
Bilirubin
- Normal Ranges:
- Adult: Total bilirubin: 0.3-1.0 mg/dL or 5.1-17 μmol/L, Indirect bilirubin: 0.2-0.8 mg/dL or 3.4-12.0 μmol/L, Direct bilirubin: 0.1-0.3 mg/dL or 1.7-5.1 μmol/L
- Newborn: Total bilirubin: 1.0-12.0 mg/dL or 17.1-205 μmol/L
- Breakdown product of heme catabolism
- Measured as direct (DBil) and total value bilirubin (TBil)
- Elevated levels (>2.5-3 mg/dL) cause jaundice and can be classified into prehepatic, hepatic, or posthepatic
- Unconjugated hyperbilirubinemia is common in newborns and can be related to higher hematocrit and decreased UGT activity
- Conjugated hyperbilirubinemia is common in individuals with hepatocellular injuries and biliary obstruction
- Normal Ranges:
-
Amylase (AMY)
- Normal Range:
- Serum Amylase: 25-129 IU/L
- Urine Amylase: 1-17 IU/L
- An enzyme produced in the pancreas and salivary glands
- Primarily used to diagnose pancreatitis, but also used for salivary gland disorders
- Lower Levels:
- Can indicate: Liver disease, cystic fibrosis, malnutrition, chronic kidney disease
- Higher Levels:
- Can indicate: Pancreatitis, salivary gland disorders, perforated peptic ulcer, bowel obstruction, or macroamylasemia
- Normal Range:
-
Aspartate Aminotransferase (AST)
- Normal Range: 10-34 IU/L
- An enzyme found in the liver and other organs
- Used to detect liver damage, but also used to monitor heart attack severity and muscle damage
- Lower Levels:
- Can indicate: Vitamin B6 deficiency
- Higher Levels:
- Can indicate: Liver damage, heart attack, muscle damage, and other conditions
-
Creatinine (CRE)
- Normal Range:
- Male: 53-106 mcmol/L
- Female: 44-80 mcmol/L
- A waste product produced by muscle metabolism
- Eliminated by the kidneys in urine
- Used to evaluate kidney function
- Lower Levels:
- Can indicate: Muscle wasting, malnutrition, or pregnancy
- Higher Levels:
- Can indicate: Kidney disease, dehydration, or muscle injury
- Normal Range:
-
Creatinine Kinase (CK)
- Normal Range:
- Male: 24-195 IU/L
- Female: 14-160 IU/L
- An enzyme found in muscle tissue
- Levels increase when muscles are injured, such as in heart attacks, muscular dystrophy, and rhabdomyolysis
- Lower Levels:
- Can indicate: Hypothyroidism
- Higher Levels:
- Can indicate: Heart attack, muscle injury (due to exercise, trauma, or genetic disorders), muscular dystrophy, rhabdomyolysis, hypothyroidism, and other conditions
- Normal Range:
-
Gamma-Glutamyl Transferase (GGT)
- Normal Range: 5-50 IU/L
- An enzyme found in the liver and other tissues
- Increased levels can indicate alcoholic liver disease or blockage of the bile ducts
- Lower Levels:
- Can indicate: Vitamin B12 deficiency
- Higher Levels:
- Can indicate: Alcohol abuse, liver disease, hepatitis, biliary obstruction, and other conditions
-
Glucose (GLU)
- Normal Range: 4.0-7.0 mmol/L
- A sugar that is the body's primary source of energy
- Used to screen for diabetes and monitor blood sugar control in people with diabetes
- Lower Levels:
- Can indicate: Hypoglycemia
- Higher Levels:
- Can indicate: Hyperglycemia, diabetes, and other conditions
-
Lactate Dehydrogenase (LD)
- Normal Range: 0.6-2.4 mmol/L
- An enzyme found in many tissues, including the heart, liver, skeletal muscles, and red blood cells
- Elevated levels can indicate tissue damage, such as in heart attacks, liver diseases, and muscular dystrophies
- Lower Levels:
- Can indicate: Anemia, Vitamin B12 deficiency
- Higher Levels:
- Can indicate: Heart attack, liver damage, muscular dystrophy, cancer, and other conditions
-
Lipase (LPS)
- Normal Range: 0-160 IU/L
- An enzyme produced in the pancreas
- Increased levels can indicate pancreatitis
- Lower Levels:
- Can indicate: Pancreatic insufficiency, malnutrition, or cystic fibrosis
- Higher Levels:
- Can indicate: Pancreatitis, acute or chronic, pancreatitis, intestinal obstruction, and other conditions
-
Phosphate (PHO)
- Normal Range: 0.8-1.5 mmol/L
- A mineral found in bone, teeth, and other tissues
- Used to evaluate kidney function, bone health, and electrolyte balance
- Lower Levels:
- Can indicate: Malnutrition, alcoholism, or vitamin D deficiency
- Higher Levels:
- Can indicate: Kidney failure, hypoparathyroidism, and other conditions
-
Potassium (POT)
- Normal Range: 3.5-5.3 mmol/L
- An electrolyte that is essential for normal nerve and muscle function
- Used to evaluate electrolyte balance and kidney function
- Lower Levels:
- Can indicate: Hypokalemia, diuretic use, and other conditions
- Higher Levels:
- Can indicate: Hyperkalemia, kidney failure, and other conditions
-
Sodium (SOD)
- Normal Range: 135-145 mmol/L
- A mineral that is essential for normal nerve and muscle function
- Used to evaluate electrolyte balance and kidney function
- Lower Levels:
- Can indicate: Hyponatremia, dehydration, low blood volume, heart failure, and other conditions
- Higher Levels:
- Can indicate: Hypernatremia, dehydration, and other conditions
-
Total Protein (TP)
- Normal Range: 60–80 g/L
- A measure of the total amount of protein in the blood
- Used to evaluate liver function, hydration status, and nutritional status
- Lower Levels:
- Can indicate: Malnutrition, liver disease, kidney disease, blood loss, or severe burns
- Higher Levels:
- Can indicate: Dehydration, multiple myeloma, and other conditions
-
Urea (URE)
- Normal Range: 2.5-8.0 mmol/L
- A waste product produced by the breakdown of protein
- Eliminated by the kidneys in urine
- Used to evaluate kidney function, hydration status, and protein intake
- Lower Levels:
- Can indicate: Liver disease, malnutrition, or pregnancy
- Higher Levels:
- Can indicate: Kidney disease, dehydration, high protein intake, or gastrointestinal bleeding
Hematology Tests
-
Complete Blood Count (CBC)
- Used to evaluate the number of red blood cells, white blood cells, and platelets in the blood.
- Red blood cells : Transport Oxygen
- White blood cells: Fight infection
- Platelets: Help stop bleeding
- CBC is a routine test for blood clotting disorders, and infections.
-
Erythrocyte Sedimentation Rate (ESR)
- Measures the rate at which red blood cells settle to the bottom of a test tube.
- A higher ESR can indicate inflammation or infection.
-
Hemoglobin (HGB)
- Measures the amount of hemoglobin in the blood. Hemoglobin is a protein in red blood cells that carries oxygen.
- Used to evaluate anemia.
-
Hematocrit (HCT)
- Measures the percentage of red blood cells in the blood.
- Used to evaluate anemia.
-
Platelet Count (PLT)
- Measures the number of platelets in the blood.
- Used to evaluate bleeding disorders.
-
White Blood Count (WBC)
- Measures the number of white blood cells in the blood.
- Used to evaluate infections.
- Higher levels can indicate infection.
Common Urine Tests
-
Urinalysis (UA)
- A routine test that checks the physical, chemical, and microscopic properties of urine.
- UA is performed in conjunction with CBCs to evaluate an individual's health status and assess various parameters including kidney function.
- Abnormal UA results can indicate infection, kidney disease, or other conditions.
-
Urine Culture
- Used to detect bacteria in the urine
- Performed when a UTI is suspected.
-
Urine Protein
- Measures the amount of protein in the urine.
- Elevated levels can indicate kidney disease.,
- It is also performed to monitor the progress of kidney disease.
-
Urine Glucose
- Measures the amount of glucose in the urine.
- Elevated levels can indicate diabetes.
-
Ketones in Urine
- Measures the amount of ketones in the urine.
- Ketones are produced when the body does not have enough glucose for fuel.
- Elevated levels can indicate diabetes or starvation.
Laboratory tests for diagnostics
- Laboratory tests are used to screen for disease, evaluate drug therapy, confirm a diagnosis, or rule out a clinical problem.
- Normal ranges are established by testing a large group of healthy individuals to determine what a typical “normal” result is.
- Reference ranges can vary depending on age and gender.
- Critical Values indicate that immediate action must be taken as the results are dangerously abnormal and may be life-threatening if corrective action is not taken.
Chemistry Tests
Albumin (AL)
- Measures the amount of albumin in the blood.
- Albumin maintains colloidal osmotic pressure.
- Normal Range: 35–50 g/L
- Indications: Used to diagnose and monitor diseases of the liver, impaired nutrition, chronic edema, and cancer.
- Interfering Factors: Drugs that interrupt normal serum electrophoretic patterns (e.g., Aspirin, bicarbonates, corticosteroids, salicylates).
Ammonia (AMM, NH3)
- Measures the amount of ammonia in the blood.
- Normal Range: 6–47 mcmol/L
- Indications: Used to diagnose severe liver diseases.
- Interfering Factors: Smoking, Eating high-protein or low-protein diet, Drugs that increase blood ammonia (e.g., Loop diuretics, narcotics, thiazides, alcohol), Drugs that decrease blood ammonia (e.g., broad spectrum antibiotics, levodopa, potassium salts), Strenuous exercise before test.
Bilirubin
- Breakdown product of heme catabolism.
- Normal Range: 0.3-1.0 mg/dL or 5.1-17 μmol/L (SI units)
- Possible critical values: Total bilirubin >12 mg/dL (Adult), Total bilirubin >15 mg/dL (Newborn)
- Elevated bilirubin levels: Elevated bilirubin levels (>2.5-3 mg/dL ) cause jaundice and can be classified into prehepatic (increased bilirubin production), hepatic (liver dysfunction), or posthepatic (duct obstruction).
Amylase (AMY)
- Normal Range: Serum Amylase:
- Indications: Used to diagnose pancreatitis, perforated peptic ulcer, intestinal obstruction, cholecystitis, salivary gland disorders (mumps, sialadenitis), and macroamylasemia.
- Interfering Factors: Recent surgery, trauma, certain drugs, and conditions such as diabetic ketoacidosis.
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Description
This quiz focuses on key chemistry tests, specifically Albumin and Ammonia levels in the blood. It explores their normal ranges, significance in diagnosing liver diseases, nutritional status, and other health conditions. Test your knowledge on the implications of abnormal results.