Podcast
Questions and Answers
If the equilibrium constant (K) for a reaction is very large, which of the following is true?
If the equilibrium constant (K) for a reaction is very large, which of the following is true?
Which of the following is NOT considered in the equilibrium constant expression?
Which of the following is NOT considered in the equilibrium constant expression?
What happens to the equilibrium constant (K) when a reaction is reversed?
What happens to the equilibrium constant (K) when a reaction is reversed?
According to the Brønsted definition, which of the following is a base?
According to the Brønsted definition, which of the following is a base?
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What is a conjugate acid-base pair?
What is a conjugate acid-base pair?
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What is an amphiprotic species?
What is an amphiprotic species?
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Which of the following is an example of a strong acid?
Which of the following is an example of a strong acid?
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Which of the following is NOT a characteristic of a strong base?
Which of the following is NOT a characteristic of a strong base?
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What is the relationship between the solubility of a solute and temperature if the enthalpy of solution (ΔHsoln) is negative?
What is the relationship between the solubility of a solute and temperature if the enthalpy of solution (ΔHsoln) is negative?
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According to Le Châtelier's Principle, what will happen to the equilibrium of a reaction if more reactants are added?
According to Le Châtelier's Principle, what will happen to the equilibrium of a reaction if more reactants are added?
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How does pressure affect the solubility of a gaseous solute in a liquid solvent?
How does pressure affect the solubility of a gaseous solute in a liquid solvent?
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What is the quantitative relationship between pressure and solubility as described by Henry's Law?
What is the quantitative relationship between pressure and solubility as described by Henry's Law?
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What is the primary application of Henry's Law in the field of anesthesia?
What is the primary application of Henry's Law in the field of anesthesia?
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What is the process called when the concentration of a volatile anesthetic in a patient is significantly increased to speed up uptake?
What is the process called when the concentration of a volatile anesthetic in a patient is significantly increased to speed up uptake?
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If the partial pressure of oxygen (PaO2) in arterial blood is 300 mmHg, how much oxygen is dissolved in 100 ml of blood?
If the partial pressure of oxygen (PaO2) in arterial blood is 300 mmHg, how much oxygen is dissolved in 100 ml of blood?
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How much does the dissolved oxygen in the blood increase when the partial pressure of oxygen (PaO2) increases from 100 mmHg to 500 mmHg?
How much does the dissolved oxygen in the blood increase when the partial pressure of oxygen (PaO2) increases from 100 mmHg to 500 mmHg?
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What is the primary characteristic of isotonic solutions?
What is the primary characteristic of isotonic solutions?
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What role does oncotic pressure play in the vascular system?
What role does oncotic pressure play in the vascular system?
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What is the effect of increasing solute concentration on osmotic pressure?
What is the effect of increasing solute concentration on osmotic pressure?
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Why is nitrous oxide contraindicated in patients with pneumothorax?
Why is nitrous oxide contraindicated in patients with pneumothorax?
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What is the primary factor that drives the process of diffusion?
What is the primary factor that drives the process of diffusion?
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Which substance does not penetrate the capillary wall but provides osmotic pressure?
Which substance does not penetrate the capillary wall but provides osmotic pressure?
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What is the primary function of a semipermeable membrane in osmosis?
What is the primary function of a semipermeable membrane in osmosis?
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In the context of capillary dynamics, what happens to fluid upon the delivery of colloids?
In the context of capillary dynamics, what happens to fluid upon the delivery of colloids?
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What is the primary function of a pH buffer solution?
What is the primary function of a pH buffer solution?
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What is the result of adding a strong base to a buffered solution?
What is the result of adding a strong base to a buffered solution?
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What happens to the unionized fraction of a weak base when placed in an environment with pH greater than its pKa?
What happens to the unionized fraction of a weak base when placed in an environment with pH greater than its pKa?
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Which molecules primarily consist of buffers in the human body?
Which molecules primarily consist of buffers in the human body?
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How does blood pH affect enzyme activity?
How does blood pH affect enzyme activity?
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What kind of reaction occurs when a strong acid is added to a buffered solution?
What kind of reaction occurs when a strong acid is added to a buffered solution?
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What role does the respiratory system play in pH balance?
What role does the respiratory system play in pH balance?
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If the pKa of a weak acid is greater than the pH of the solution, what fraction predominates?
If the pKa of a weak acid is greater than the pH of the solution, what fraction predominates?
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What is the primary function of metabolism in cells?
What is the primary function of metabolism in cells?
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Which type of reaction is dehydration synthesis?
Which type of reaction is dehydration synthesis?
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Which of the following statements about ATP is true?
Which of the following statements about ATP is true?
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What role does insulin play in glucose transport?
What role does insulin play in glucose transport?
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What is the common pathway for the transport of carbohydrates into cells?
What is the common pathway for the transport of carbohydrates into cells?
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During the process of phosphorylation, what compound is formed when glucose enters the cell?
During the process of phosphorylation, what compound is formed when glucose enters the cell?
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What differentiates aerobic metabolism from anaerobic metabolism in terms of ATP production?
What differentiates aerobic metabolism from anaerobic metabolism in terms of ATP production?
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Which of the following best describes catabolism?
Which of the following best describes catabolism?
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What describes the behavior of weak acids when dissolved in water?
What describes the behavior of weak acids when dissolved in water?
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How do polyprotic acids, like diprotic acids, behave in terms of donating hydrogen ions?
How do polyprotic acids, like diprotic acids, behave in terms of donating hydrogen ions?
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What is the conjugate base of a strong acid typically characterized by?
What is the conjugate base of a strong acid typically characterized by?
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In an acid-base reaction, what happens to the acid and base?
In an acid-base reaction, what happens to the acid and base?
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Which statement about weak bases is correct?
Which statement about weak bases is correct?
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When comparing the strength of conjugate acid-base pairs, which relationship is accurate?
When comparing the strength of conjugate acid-base pairs, which relationship is accurate?
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Which of the following is true regarding the charges of acids and bases in a reaction?
Which of the following is true regarding the charges of acids and bases in a reaction?
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Which characteristic does not apply to polyprotic acids?
Which characteristic does not apply to polyprotic acids?
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Which of the following factors can affect chemical equilibrium?
Which of the following factors can affect chemical equilibrium?
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Which of the following factors can affect the position of equilibrium in a chemical reaction?
Which of the following factors can affect the position of equilibrium in a chemical reaction?
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Which of the following statements correctly describes the equilibrium constant (K) when it decreases?
Which of the following statements correctly describes the equilibrium constant (K) when it decreases?
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What is the relationship between pH and pOH in an aqueous solution at 25°C?
What is the relationship between pH and pOH in an aqueous solution at 25°C?
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What is the effect of dissolving carbon dioxide in water on the pH of the solution?
What is the effect of dissolving carbon dioxide in water on the pH of the solution?
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Which of the following is NOT true about the pH scale?
Which of the following is NOT true about the pH scale?
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What is the primary reason for the slight alteration in hemoglobin structure in cellular tissue with high carbon dioxide concentration?
What is the primary reason for the slight alteration in hemoglobin structure in cellular tissue with high carbon dioxide concentration?
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What is the relationship between pKa and pKb for a conjugate acid-base pair?
What is the relationship between pKa and pKb for a conjugate acid-base pair?
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What is the primary role of nonmetal oxides in the context of acidity?
What is the primary role of nonmetal oxides in the context of acidity?
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Which of the following statements accurately describes the behavior of carbonic acid (H2CO3) in an aqueous solution?
Which of the following statements accurately describes the behavior of carbonic acid (H2CO3) in an aqueous solution?
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How does the buildup of carbon dioxide in the blood contribute to acidosis?
How does the buildup of carbon dioxide in the blood contribute to acidosis?
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Which type of phospholipid is a component of myelin sheaths surrounding nerve cells?
Which type of phospholipid is a component of myelin sheaths surrounding nerve cells?
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What is a primary consequence of atherosclerosis in large arteries?
What is a primary consequence of atherosclerosis in large arteries?
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Which substance is essential for the synthesis of cholesterol and is inhibited by statins?
Which substance is essential for the synthesis of cholesterol and is inhibited by statins?
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Which factor is NOT associated with leading to atherosclerosis?
Which factor is NOT associated with leading to atherosclerosis?
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Which of the following hormones can be synthesized from cholesterol?
Which of the following hormones can be synthesized from cholesterol?
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What is a significant feature of regulatory proteins like G Protein Coupled Receptors?
What is a significant feature of regulatory proteins like G Protein Coupled Receptors?
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Which of the following dietary changes is considered most important in the prevention of atherosclerosis?
Which of the following dietary changes is considered most important in the prevention of atherosclerosis?
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What is the process by which fatty acids are converted to Acetyl-CoA?
What is the process by which fatty acids are converted to Acetyl-CoA?
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What percentage of body solids are made up of proteins?
What percentage of body solids are made up of proteins?
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What are ketone bodies primarily produced from during periods of carbohydrate scarcity?
What are ketone bodies primarily produced from during periods of carbohydrate scarcity?
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What role does lipoprotein lipase play in the metabolism of triglycerides?
What role does lipoprotein lipase play in the metabolism of triglycerides?
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What happens to triglycerides in fat cells when there is an increased need for lipids in the body?
What happens to triglycerides in fat cells when there is an increased need for lipids in the body?
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Which hormone is responsible for activating triglyceride lipase to increase free fatty acids in the blood?
Which hormone is responsible for activating triglyceride lipase to increase free fatty acids in the blood?
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What is the primary storage form of fat in adipose tissue?
What is the primary storage form of fat in adipose tissue?
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What percentage of calories from fats is typical in the American diet?
What percentage of calories from fats is typical in the American diet?
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What metabolic pathway does glycerol enter after being converted from triglycerides?
What metabolic pathway does glycerol enter after being converted from triglycerides?
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What is the primary role of albumin in the bloodstream?
What is the primary role of albumin in the bloodstream?
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Which type of amino acids must be obtained from the diet due to the body's inability to synthesize them?
Which type of amino acids must be obtained from the diet due to the body's inability to synthesize them?
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What occurs during the process of deamination?
What occurs during the process of deamination?
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What happens to amino acids when their concentrations exceed the renal threshold for reabsorption?
What happens to amino acids when their concentrations exceed the renal threshold for reabsorption?
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What is the consequence of consuming less than 20-30 g of protein per day?
What is the consequence of consuming less than 20-30 g of protein per day?
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Which enzymes are primarily responsible for the deamination of amino acids?
Which enzymes are primarily responsible for the deamination of amino acids?
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What is ketogenesis?
What is ketogenesis?
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What stimulates the transport of amino acids into cells?
What stimulates the transport of amino acids into cells?
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What is the primary function of glycogenolysis?
What is the primary function of glycogenolysis?
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How many ATP molecules are produced during glycolysis from one mole of glucose?
How many ATP molecules are produced during glycolysis from one mole of glucose?
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Which compound is formed when Acetyl-CoA combines with oxaloacetic acid in the Citric Acid Cycle?
Which compound is formed when Acetyl-CoA combines with oxaloacetic acid in the Citric Acid Cycle?
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What occurs during the process of oxidative phosphorylation?
What occurs during the process of oxidative phosphorylation?
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In glycolysis, glucose is split into how many molecules of pyruvic acid?
In glycolysis, glucose is split into how many molecules of pyruvic acid?
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What is the efficiency of ATP formation per mole of glucose during glycolysis?
What is the efficiency of ATP formation per mole of glucose during glycolysis?
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What is the net reaction produced in the Citric Acid Cycle per molecule of glucose?
What is the net reaction produced in the Citric Acid Cycle per molecule of glucose?
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What is the approximate number of calories released from one gram-mole of glucose during its oxidation?
What is the approximate number of calories released from one gram-mole of glucose during its oxidation?
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Flashcards
Metabolism
Metabolism
The chemical processes that allow cells to live by converting food energy into usable forms.
Anabolism
Anabolism
A metabolic process that involves the synthesis of complex molecules from simpler ones, requiring energy input.
Catabolism
Catabolism
A metabolic process that involves the breakdown of complex molecules into simpler ones, releasing energy.
Dehydration Synthesis
Dehydration Synthesis
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Hydrolysis
Hydrolysis
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ATP
ATP
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Glucose Transport
Glucose Transport
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Phosphorylation
Phosphorylation
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ΔHsoln
ΔHsoln
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Le Châtelier’s Principle
Le Châtelier’s Principle
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Effect of Pressure on Gas Solubility
Effect of Pressure on Gas Solubility
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Henry's Law
Henry's Law
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Overpressurizing
Overpressurizing
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Dissolved O2 Calculation
Dissolved O2 Calculation
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Total O2 Dissolved Increase
Total O2 Dissolved Increase
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Gas Compressibility
Gas Compressibility
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Tonicity
Tonicity
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Isotonic Solutions
Isotonic Solutions
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Osmosis
Osmosis
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Osmotic Pressure
Osmotic Pressure
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Oncotic Pressure
Oncotic Pressure
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Colligative Properties
Colligative Properties
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Diffusion
Diffusion
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Apneic Oxygenation
Apneic Oxygenation
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Equilibrium Constant (K)
Equilibrium Constant (K)
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Kforward and Kreverse
Kforward and Kreverse
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Arrhenius Acid Definition
Arrhenius Acid Definition
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Arrhenius Base Definition
Arrhenius Base Definition
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Brønsted Acid
Brønsted Acid
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Brønsted Base
Brønsted Base
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Conjugate Acid-Base Pair
Conjugate Acid-Base Pair
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Strong Acids
Strong Acids
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Weak Acids
Weak Acids
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Weak Bases
Weak Bases
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Polyprotic Acids
Polyprotic Acids
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Acid-Base Reactions
Acid-Base Reactions
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Dynamic Equilibrium
Dynamic Equilibrium
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Diprotic Acid
Diprotic Acid
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Triprotic Acid
Triprotic Acid
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pH Buffer
pH Buffer
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Weak Acid and Conjugate Base
Weak Acid and Conjugate Base
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Henderson–Hasselbalch Equation
Henderson–Hasselbalch Equation
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Ionized vs Unionized
Ionized vs Unionized
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Respiratory System and pH Balance
Respiratory System and pH Balance
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Role of Buffers
Role of Buffers
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Hydrogen Ion Interaction
Hydrogen Ion Interaction
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Acid-Base Disturbances
Acid-Base Disturbances
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Study Notes
Chemical Reactions and Metabolism
- Metabolism describes the chemical processes that keep cells alive.
- Anabolism is the synthesis of molecules, whereas catabolism is the breakdown of molecules.
- Energy production involves oxidation of proteins, carbohydrates, and fats to make ATP.
- Energy usage involves processes like active transport, muscle contraction, synthesis of molecules, and cell growth.
- Chemical energy in food must be released slowly to fuel cellular processes like membrane pumps, protein synthesis, and muscle contractions.
Carbohydrate Metabolism and Formation of ATP
- Glucose (C6H12O6) is the most important carbohydrate for energy transport into cells.
- After absorption, most fructose and galactose convert to glucose in the liver.
- Glucose enters the cell through facilitated diffusion with a carrier protein via cotransport or facilitated diffusion.
- Phosphorylation converts glucose to glucose-6-phosphate (immediately used or stored as glycogen).
- Glycogenesis forms glycogen (a starch).
- Glycogenolysis breaks glycogen into glucose.
- Glycolysis splits glucose into two pyruvate molecules, producing 2 ATP + 4 H.
- Efficiency of ATP formation during glycolysis is 43%.
- Pyruvate molecules combined with Coenzyme A to form Acetyl-CoA.
- Krebs Cycle, also known as Citric Acid Cycle, starts with Acetyl CoA combines with oxaloacetic acid to form citric acid.
- Krebs Cycle releases 24 hydrogen atoms per molecule of glucose, forming up to 24 ATP later.
- Oxidative phosphorylation, consisting of Electron Transport Chain & chemiosmosis, is oxygen-dependent, generating 34 more ATPs.
- Aerobic metabolism yields much more ATP than anaerobic.
- Total ATP production from glucose: 38 ATP.
Energy Foods
- Fats, carbohydrates, and proteins can be oxidized by cells to release large amounts of energy.
- Phosphate bonds in ATP have high energy content.
Coupled Reactions
- Chemical energy in foods must be released slowly.
- Energy release is coupled with cellular processes like membrane pumps and protein synthesis.
ATP - Adenosine Triphosphate
- ATP transfers energy between coupled reactions.
- Present throughout the cell, storing energy in high-energy bonds.
- Each high-energy bond in a mole of ATP stores 12,000 calories.
Glucose (Dextrose)
- After absorption from the GI tract, fructose and galactose are converted to glucose in the liver.
- Glucose is the final common pathway for carbohydrate transport into cells.
Transport of Glucose Through Cell Membrane
- Active co-transport or facilitated diffusion with a carrier protein is necessary.
- Insulin facilitates this process.
Phosphorylation
- Immediately upon entering cells, glucose combines with a phosphate radical to form glucose-6-phosphate.
- Glucose-6-phosphate is used immediately for cellular energy or stored as glycogen.
Glycogenesis
- Formation of glycogen, a starch.
Glycogenolysis
- Breakdown of glycogen to glucose in the liver.
- Makes glucose available, using phosphorylation via phosphorylase.
- Hormones (epinephrine and glucagon) activate phosphorylase.
Release of Energy from Glucose
- Glycolysis, the citric acid cycle, and the electron transport chain release energy from glucose.
Glycolytic Pathway
- One gram-mole of glucose releases 686,000 calories, and only 12,000 calories are needed to form one gram-mole of ATP.
- Enzymes oxidize glucose in small steps, maximizing energy capture as ATP.
- Energy is released in packets, yielding 38 ATP per mole of glucose.
Glycolysis
- The most important process for releasing energy from glucose
- Involves 10 steps
- Ends with the formation of 2 pyruvic acid molecules + 2 ATP + 4 H.
- Happens in cytoplasm.
- 2 Pyruvic acid molecules combine with Coenzyme A to form Acetyl-CoA.
Citric Acid Cycle (Krebs Cycle)
- Occurs in the matrix of mitochondria.
- Acetyl CoA combines with oxaloacetic acid to form citric acid.
- The cycle begins and ends with oxaloacetic acid production.
- Releases 24 hydrogen atoms per glucose molecule, leading to ATP generation.
Citric Acid Cycle (Krebs Cycle) (Hydrogen atoms)
- 20 hydrogen atoms combine with NAD+ via dehydrogenase.
- Carbon dioxide and hydrogen atoms are released during the next stages.
- 2 molecules of ATP are produced.
Net Reaction per Molecule of Glucose in Citric Acid Cycle
- The reaction can be summarized as: 2 Acetyl-CoA + 6 H2O + 2 ADP → 4 CO2 + 16 H+ + 2 CoA + 2 ATP.
Oxidative Phosphorylation
- Oxygen is needed.
- NADH splits into NAD+, H+, and e- by hydrogen oxidation.
- Electrons enter the electron transport chain.
- Energy released is captured as a proton gradient to create ATP.
- The entire process is called chemiosmosis.
Summary of ATP Formation
- Glycolysis yields 4 ATP (2 used), and 2 ATP from the Krebs Cycle, 34 ATP from the Electron Transport Chain.
- In total 38 ATP from one glucose molecule.
- Glucose oxidation is approximately 66% efficient (in comparison to total energy released).
- Feedback controls start or stop ATP formation.
Anaerobic Glycolysis
- Results from a lack of oxygen.
- Pyruvic acid + NADH + H+ → lactic acid.
- Measuring lactic acid in the OR reflects cell oxygen usage.
- When oxygen is available, lactic acid re-enters the glucose pathway to produce energy.
Storage of Glucose
- Glycogen stores fill up first, followed by fat storage.
Gluconeogenesis
- When glucose isn't available, it's synthesized from fats (glycerol) and proteins (amino acids).
- Low blood glucose triggers the release of cortisol.
- Cortisol promotes protein breakdown to amino acids, suitable for liver glucose conversion.
- This is a catabolic process.
Lipid Metabolism
- Lipids are cellular fuels, with high energy content.
- Lipids are the most efficient form of cellular energy storage.
- Major lipids include triglycerides, phospholipids, and cholesterol.
Fatty Acids
- Fatty acids are simple, long-chain hydrocarbon carboxylic acids.
- They contain the carboxyl group (-COOH).
- Examples of fatty acids include Palmitic acid (CH3(CH2)14COOH).
Triglycerides
- Triglycerides are three long-chain fatty acid molecules attached to a glycerol molecule.
- Glycerol is a triple alcohol (OH).
- Tristearin is an example.
Absorption of Fats
- Dietary triglycerides are broken down into monoglycerides and fatty acids in the intestines.
- These are repackaged as chylomicrons, absorbed into the lymphatic system.
- Plasma becomes turbid following a high-fat meal.
Uptake Into Cells
- Lipoprotein lipase within capillary endothelium (liver cells) hydrolyzes triglycerides into fatty acids and glycerol.
- Fatty acids diffuse into cells.
- Once inside, fatty acids are resynthesized into triglycerides for storage.
Transport Through Body
- Fat cells hydrolyze triglycerides into fatty acids and glycerol when lipids are needed.
- These components enter the bloodstream and combine with albumin to form free fatty acids.
Fat Deposits
- Adipose tissue is called fat deposits or simple tissue fat.
- Fats are also stored in the liver (potentially leading to non-alcoholic steatohepatitis (NASH)).
- Adipose tissue fat cells are typically 80–95% triglycerides.
- Fat deposits are responsive to hormonal and other factors.
Use of Triglycerides for Energy
- Up to 50% of the calories in the typical American diet come from fats.
- Triglycerides are hydrolyzed into fatty acids and glycerol.
- Glycerol is transformed into glycerol-3-phosphate and enters the glycolytic pathway for glucose utilization.
- Fatty acids enter mitochondria for oxidation.
Beta-Oxidation
- Fatty acids are converted to acetyl-CoA through beta-oxidation, a multi-step process.
- Acetyl-CoA enters the citric acid cycle in carbohydrate metabolism.
- Beta-oxidation of stearic acid produces 148 molecules of ATP (after initial 2 ATP use, leaving a 146 net gain).
Ketosis
- Fats are oxidized to fuel the body when carbohydrates are unavailable (like in the Atkins diet).
- High concentrations of B-hydroxybutyric acid, acetoacetic acid, and acetone form ketone bodies.
- Causes include starvation, diabetes mellitus, and high-fat, low-carb diets.
Regulation of Fat Utilization
- Epinephrine and norepinephrine activate triglyceride lipase, increasing free fatty acid production.
- Corticotropin and glucocorticoid release (from the anterior pituitary and adrenal cortex) increase fatty acid use.
- Insulin release decreases.
Phospholipids
- Phospholipids contain a fatty acid molecule, phosphoric acid radical, and a nitrogenous base.
- Three types are lecithins, cephalins, and sphingomyelins.
Uses of Phospholipids
- Essential components of cell membranes.
- Found in lipoproteins.
- Involved in clotting (thromboplastin).
- Constituents of myelin sheaths in nerve cells.
- Act as phosphate radical donors.
Uses of Cholesterol
- Forms cholic acid (bile) for fat digestion.
- Converted into adrenocortical hormones like cortisol.
- Precursor for sex hormones (estrogen, progesterone, and testosterone).
- Forms a waterproof layer in the skin (corneum).
Atherosclerosis
- A disease characterized by fatty lesions (atheromatous plaques) accumulating in large arteries.
- Arterosclerosis leads to vessel thickening and stiffening.
- Plaques are mainly composed of cholesterol.
- Connective tissue within plaques causes vessel wall stiffening (sclerotic).
- Plaque can occlude vessels, causing rupture.
Factors that Lead to Atherosclerosis
- Physical inactivity and obesity.
- Familial history.
- Diabetes mellitus.
- High blood pressure (hypertension).
- High blood lipids (hyperlipidemia).
- Smoking.
Prevention of Atherosclerosis
- Consume a low-fat diet (most important).
- Do not smoke.
- Exercise regularly.
- Manage blood pressure.
- Manage blood glucose levels.
- Include oat bran in diet to bind bile acids in the gut.
- Statins inhibit HGM-CoA reductase and reduce cholesterol synthesis.
Protein Metabolism
- Proteins, comprising 3/4th of body solids, are made from 20 amino acids linked by peptide bonds and hydrogen bonds.
- Structural components, enzymes, oxygen transport, nucleoproteins, muscle contraction, and cellular functions are protein-dependent.
Regulatory Protein
- Regulatory proteins transmit messages from a chemical signal to other parts of cells.
- G protein-coupled receptors transmit messages through cell membranes.
- Ligands bind to receptors, activating G protein-linked intracellular events.
- These messages lead to communication between cells.
Transport of Amino Acids
- Proteins are digested into amino acids in the gastrointestinal tract.
- Protein digestion/absorption takes 2-3 hours.
- Active transport and facilitated diffusion move amino acids into cells.
- Excess amino acids exceed renal threshold and excretion occurs in urine.
Storage of Amino Acids
- Amino acids immediately enter cells after digestion, becoming new proteins.
- Low blood amino acid levels trigger their transport out of the cells.
Major Plasma Proteins
- Albumin - colloid osmotic pressure
- Globulins - enzymes, immune system
- Fibrinogen - coagulation
Dietary Amino Acids
- 10 essential amino acids must be ingested, as the body cannot synthesize them.
- 10 other (nonessential) amino acids can be synthesized by the body, but adequate amounts are needed for protein synthesis.
Use of Proteins for Energy
- Protein breakdown, or deamination, occurs in the liver.
- Removal of the amino group (NH2) from amino acids.
- Aminotransferases are enzymes responsible for deamination.
Urea
- Ammonia, a neurotoxin from deamination, is converted to urea in the liver for removal from the blood.
- Urea is excreted by the kidneys.
Oxidation of Deaminated Amino Acids
- Deaminated keto acids are degraded into substances that can enter the citric acid cycle.
- These substances are oxidized to produce ATP.
- This conversion to keto acids or fatty acids is called ketogenesis.
Obligatory Degradation of Proteins
- 20-30 g of protein is degraded to amino acids and oxidized daily.
- Protein intake less than that level leads to starvation. - Carbohydrates are protein sparers.
Hormonal Regulation of Protein Metabolism
- Growth hormone promotes amino acid transport into cells for protein synthesis.
- Insulin increases amino acid transport into cells.
- Glucocorticoids increase the breakdown of extrahepatic proteins.
- Testosterone promotes protein deposition in muscle tissue.
- Thyroxine (T4) increases the rate of metabolism.
Aqueous Solutions & Concentrations: Solubility, Diffusion, and Osmosis
- Definitions and types of solutions.
- Solubility is the amount of one substance that dissolves in another at a particular temperature.
- Diffusion is the net movement of molecules down their concentration gradient.
- Osmosis is the movement of water across a semipermeable membrane in response to concentration.
Solution Concentrations
- Molarity: moles of solute per liter of solution.
- Molality: moles of solute per kilogram of solvent.
- Percent by weight to volume (%w/v): grams of solute per 100 mL solution.
- Percent by volume (%v/v): milliliters of solute per 100 ml of solution.
- Normality (acid-base): equivalents of solute per liter of solution; analogous to molarity (but based on chemical reactivity, not mass).
- Parts per million (ppm): grams of solute per 1,000,000 grams of solution.
Solubility
- Solubility is enhanced by similar intermolecular interactions between substances.
- "Like dissolves like" (polar dissolves polar; nonpolar dissolves nonpolar).
- Factors affecting solubility include intermolecular interactions, temperature, and pressure.
- A saturated solution contains the maximum amount of a solute in a given solvent at a given temperature.
Solubility & Temperature
- Solubility typically increases with increasing temperature for solids and liquids but decreases for gases.
Energy Changes & the Solution Process
- Breaking bonds requires energy (endothermic); Forming bonds releases energy (exothermic).
- Heat of solution is the energy change during solute dissolving in a solvent.
- The enthalpy of solution (ΔHsoln) is numerically equal to the heat change, if the pressure remains constant.
Energy Changes & the Solution Process
- The solution process can be endothermic or exothermic, depending on the relative energies of bond breaking and bond formation.
- Le Châtelier's principle states that systems at equilibrium respond to stress by shifting to restore equilibrium.
Colligative Properties of Solutions
- Vapor pressure depression describes how solute particles hinder solvent molecule escape, decreasing solution vapor pressure.
- Boiling point elevation is the resultant increase in boiling temperature caused by solute presence.
- Freezing point depression is the drop in freezing temperature due to solute interference.
- Diffusion is the net movement of molecules to minimize concentration gradients.
- Fick's law quantifies gas diffusion across membranes, considering partial pressure gradients, membrane solubility, area, thickness, and molecular weight.
- Osmosis is water movement across semipermeable membranes due to solute concentration differences.
- Osmotic and oncotic pressures balance fluid forces in capillary exchange. Differences in these pressures regulate fluid movement between blood and tissues.
Other Acidic Species
- Nonmetal oxides dissolved in water forms acidic solutions.
- CO2 is a critical example, forming carbonic acid (H2CO3) when dissolved in water, impacting blood pH and oxygen transport.
Buffers
- A buffer solution maintains a stable pH when acids or bases are added.
- It contains a weak acid and its conjugate base or a weak base and its conjugate acid.
- Buffers resist changes in pH by neutralizing added acids or bases.
Henderson-Hasselbalch Equation
- The Henderson-Hasselbalch equation calculates the pH of a buffer solution considering the pKa of the weak acid and the ratio of the concentrations of the weak acid and its conjugate base.
- The pH relative to the pKa determines whether the ionized or unionized form of a weak acid or base predominates.
- The degree of ionization of a molecule increases as the pKa moves further from the physiological pH.
Acid-Base Balance & Respiratory System
- The respiratory system and kidneys work together to maintain normal blood pH.
- The primary buffers are bicarbonate, phosphate, and proteins.
- Key variables for assessing acid-base balance are blood pH, PaCO2 (partial pressure of carbon dioxide) and HCO3 (bicarbonate).
- The respiratory system compensates for metabolic acid/base disturbances by altering ventilation.
Other Important Concepts
- Anion gap calculation helps evaluate the cause of metabolic acidosis.
- Interpretation of blood gases (ABGs) clarifies acid-base imbalances.
- Treatment for blood gas abnormalities involves adjusting ventilation for respiratory issues and addressing circulatory problems for metabolic issues.
Diffusion/Anesthesia
- Diffusion is a passive process driven by entropy.
- Gases/liquids distribute uniformly over time.
- Diffusion rates depend on the medium.
- Nitrous oxide's diffusion into air-filled cavities is relevant clinically and can cause tissue damage.
- Fick's Law describes gas diffusion across biological tissues, impacted by partial pressure, membrane solubility, area, thickness, and molecular weight.
- Understanding Fick's law is crucial for a variety of medical scenarios.
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Description
Test your understanding of equilibrium constants, acid-base theories, and solubility principles in this comprehensive chemistry quiz. It covers key concepts such as the Brønsted definition, Le Châtelier's Principle, and Henry's Law. Evaluate your knowledge of strong acids, bases, and the effects of temperature and pressure on solubility.