16 Questions
What is the Michaelis Menten constant (Km) defined as?
The substrate concentration that gives 50% of the maximum velocity
What does Vmax represent in the Michaelis Menten model?
The maximum velocity that can be obtained when the enzyme is saturated by the substrate
How is the Michaelis Menten Model different from the time course of an enzymatic reaction graph?
The MM graph includes time and product on the x and y axes, whereas the time course graph includes substrate concentration and reaction velocity
What can be inferred from a high Vmax in an enzyme-catalyzed reaction?
The enzyme has high catalytic activity
In which scenario can Vmax be achieved in an enzyme-catalyzed reaction?
At excessively high concentration of substrates
How is the Michaelis Menten graph used to understand enzyme kinetics?
It illustrates how the rate of an enzymatic reaction depends on the concentration of the enzyme and substrate
What is the purpose of plotting the reciprocal of the MM graph?
To find the Vmax
What information does the Michaelis Menten constant (Km) provide about the enzyme kinetics?
Affinity of the enzymes for the substrate
What is the definition of turnover number/catalytic constant (Kcat)?
The number of substrate molecules converted into product in a unit time by one enzyme molecule
What happens when the enzyme is totally saturated by the substrate, in relation to Kcat?
Kcat remains constant
How can Vmax be found using the MM graph?
By finding the x-intercept of the MM graph
What is the relationship between K2 and Kcat in the equilibrium equation?
They are equal in value
What does the specificity constant describe?
The ratio between Kcat and Km
Why is it difficult to evaluate the Michaelis Menten constant (Km) using the MM graph?
Vmax is an unknown asymptote
What aspect of enzyme performance does a low Km indicate?
High affinity for substrate
'Highly efficient enzymes have a high Kcat and a low Km' - What does this statement imply?
Km is inversely proportional to Kcat
Explore the regulation and biological catalysts of enzymes in this lecture covering the Michaelis Menten Model and its implications on enzyme kinetics. Understand the relationship between enzyme and substrate concentrations and the concept of Vmax.
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