Podcast
Questions and Answers
What is the Michaelis Menten constant (Km) defined as?
What is the Michaelis Menten constant (Km) defined as?
- The substrate concentration that gives 50% of the maximum velocity (correct)
- The time required for the enzyme to catalyze a reaction
- The concentration of the enzyme in a saturated solution
- The maximum velocity of the enzyme-substrate reaction
What does Vmax represent in the Michaelis Menten model?
What does Vmax represent in the Michaelis Menten model?
- The amount of substrate required for the enzyme to catalyze a reaction
- The maximum velocity that can be obtained when the enzyme is saturated by the substrate (correct)
- The maximum enzyme concentration in a reaction
- The time taken for the enzyme to reach its maximum activity
How is the Michaelis Menten Model different from the time course of an enzymatic reaction graph?
How is the Michaelis Menten Model different from the time course of an enzymatic reaction graph?
- The MM graph includes time and product on the x and y axes, whereas the time course graph includes substrate concentration and reaction velocity (correct)
- The MM graph represents the rate of an enzymatic reaction, whereas the time course graph represents enzyme concentration
- The MM graph is used to calculate Km, whereas the time course graph is used to calculate Vmax
- The MM graph shows the initial velocity, whereas the time course graph shows the final velocity
What can be inferred from a high Vmax in an enzyme-catalyzed reaction?
What can be inferred from a high Vmax in an enzyme-catalyzed reaction?
In which scenario can Vmax be achieved in an enzyme-catalyzed reaction?
In which scenario can Vmax be achieved in an enzyme-catalyzed reaction?
How is the Michaelis Menten graph used to understand enzyme kinetics?
How is the Michaelis Menten graph used to understand enzyme kinetics?
What is the purpose of plotting the reciprocal of the MM graph?
What is the purpose of plotting the reciprocal of the MM graph?
What information does the Michaelis Menten constant (Km) provide about the enzyme kinetics?
What information does the Michaelis Menten constant (Km) provide about the enzyme kinetics?
What is the definition of turnover number/catalytic constant (Kcat)?
What is the definition of turnover number/catalytic constant (Kcat)?
What happens when the enzyme is totally saturated by the substrate, in relation to Kcat?
What happens when the enzyme is totally saturated by the substrate, in relation to Kcat?
How can Vmax be found using the MM graph?
How can Vmax be found using the MM graph?
What is the relationship between K2 and Kcat in the equilibrium equation?
What is the relationship between K2 and Kcat in the equilibrium equation?
What does the specificity constant describe?
What does the specificity constant describe?
Why is it difficult to evaluate the Michaelis Menten constant (Km) using the MM graph?
Why is it difficult to evaluate the Michaelis Menten constant (Km) using the MM graph?
What aspect of enzyme performance does a low Km indicate?
What aspect of enzyme performance does a low Km indicate?
'Highly efficient enzymes have a high Kcat and a low Km' - What does this statement imply?
'Highly efficient enzymes have a high Kcat and a low Km' - What does this statement imply?
