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Questions and Answers
What is the primary advantage of measuring the increase in product rather than the decrease in substrate in enzyme reactions?
What is the primary advantage of measuring the increase in product rather than the decrease in substrate in enzyme reactions?
What is the effect of increasing the ionic strength of a solution on enzyme activity?
What is the effect of increasing the ionic strength of a solution on enzyme activity?
Which of the following is NOT a suitable anticoagulant for measuring enzyme activity in a blood sample?
Which of the following is NOT a suitable anticoagulant for measuring enzyme activity in a blood sample?
What is the effect of a 10°C increase in temperature on enzyme activity?
What is the effect of a 10°C increase in temperature on enzyme activity?
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What type of enzyme inhibitor competes with the substrate for binding to the active site of the enzyme?
What type of enzyme inhibitor competes with the substrate for binding to the active site of the enzyme?
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What is the pH range in which most enzyme reactions occur?
What is the pH range in which most enzyme reactions occur?
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What type of inhibitor binds to an enzyme different from the catalytic site, removing cofactors in the process?
What type of inhibitor binds to an enzyme different from the catalytic site, removing cofactors in the process?
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Which class of enzyme catalyzes the transfer of a group, other than hydrogen, from one substrate to another?
Which class of enzyme catalyzes the transfer of a group, other than hydrogen, from one substrate to another?
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What is the purpose of the last number in the Enzyme Commission of the International Union of Biochemistry standardized designation?
What is the purpose of the last number in the Enzyme Commission of the International Union of Biochemistry standardized designation?
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What type of enzyme catalyzes the removal of groups from substrates without hydrolysis, resulting in a double bond product?
What type of enzyme catalyzes the removal of groups from substrates without hydrolysis, resulting in a double bond product?
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What is the function of an uncompetitive inhibitor?
What is the function of an uncompetitive inhibitor?
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What is the primary function of ligase enzymes?
What is the primary function of ligase enzymes?
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What is the primary advantage of using Alanine Aminotransferase (ALT) over Aspartate Aminotransferase (AST) in diagnosing liver disease?
What is the primary advantage of using Alanine Aminotransferase (ALT) over Aspartate Aminotransferase (AST) in diagnosing liver disease?
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Which of the following methods is used to detect Alanine Aminotransferase (ALT) activity?
Which of the following methods is used to detect Alanine Aminotransferase (ALT) activity?
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What is the main difference between the substrate used in the Bodansky method and the Shinowara Jones method?
What is the main difference between the substrate used in the Bodansky method and the Shinowara Jones method?
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Why do ALT elevations tend to remain longer than AST elevations in acute inflammatory conditions of the liver?
Why do ALT elevations tend to remain longer than AST elevations in acute inflammatory conditions of the liver?
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What is the primary concern when measuring ALT activity in patients with viral hepatitis?
What is the primary concern when measuring ALT activity in patients with viral hepatitis?
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Which of the following is a characteristic of AST that distinguishes it from ALT?
Which of the following is a characteristic of AST that distinguishes it from ALT?
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What is the effect on reaction speed when the substrate concentration is doubled in a first-order reaction?
What is the effect on reaction speed when the substrate concentration is doubled in a first-order reaction?
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Which type of inhibitor binds to the active site of the enzyme?
Which type of inhibitor binds to the active site of the enzyme?
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What is the unit of enzyme activity that represents the amount of enzyme needed to catalyze the conversion of 1 mole of substrate to products per second?
What is the unit of enzyme activity that represents the amount of enzyme needed to catalyze the conversion of 1 mole of substrate to products per second?
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What is the conversion rate of 1 IU to kat?
What is the conversion rate of 1 IU to kat?
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What is the method of measuring enzyme activity that involves measuring the rate of disappearance of substrate per unit time?
What is the method of measuring enzyme activity that involves measuring the rate of disappearance of substrate per unit time?
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What is the term for the substrate that is acted upon by the enzyme and is incubated with enzymes present in the patient's serum?
What is the term for the substrate that is acted upon by the enzyme and is incubated with enzymes present in the patient's serum?
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What is the primary reason for separating serum from clot quickly in LD isoenzyme measurement?
What is the primary reason for separating serum from clot quickly in LD isoenzyme measurement?
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In which condition is a flipped pattern of LD1 > LD2 typically observed?
In which condition is a flipped pattern of LD1 > LD2 typically observed?
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What is the primary isoenzyme fraction in the sera of healthy individuals?
What is the primary isoenzyme fraction in the sera of healthy individuals?
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Which of the following isoenzymes is NOT typically used to test liver disorders?
Which of the following isoenzymes is NOT typically used to test liver disorders?
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What is the primary reason for avoiding hemolysis in LD isoenzyme measurement?
What is the primary reason for avoiding hemolysis in LD isoenzyme measurement?
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What is the primary characteristic of LD5 isoenzyme in terms of temperature?
What is the primary characteristic of LD5 isoenzyme in terms of temperature?
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