Chemical Safety in Laboratories
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Questions and Answers

What is the primary reason for adhering to chemical safety protocols in a laboratory?

  • To prevent accidents and ensure everyone's safety (correct)
  • To impress visitors
  • To finish experiments more quickly
  • To make the lab look professional
  • Which of the following is considered appropriate Personal Protective Equipment (PPE) to wear in a chemical laboratory?

  • Any comfortable mask, gloves, and regular clothing
  • Sunglasses, open-toe shoes, and a lab coat
  • Chemical splash goggles, chemical resistant apron, closed-toe shoes, and safety gloves (correct)
  • Surgical mask, sandals, and a cotton apron
  • Which of the following activities is NOT allowed in a chemical lab?

  • Carefully handling laboratory glassware
  • Following approved experimental procedures
  • Engaging in practical jokes (correct)
  • Reporting all spills immediately to the teacher
  • What is the correct procedure for using a pipette in the laboratory?

    <p>Use a pipetted aid or suction bulb (B)</p> Signup and view all the answers

    What should you do immediately after an accident or injury occurs in the lab?

    <p>Report it to your teacher immediately (D)</p> Signup and view all the answers

    What should be done with coats, bags, and other personal items in the laboratory?

    <p>They should be placed in designated areas away from benches and aisles (C)</p> Signup and view all the answers

    When should heating apparatus, gas valves, and water faucets be turned off?

    <p>Whenever they are not in use (B)</p> Signup and view all the answers

    What is one important precaution to take when working with hot glassware?

    <p>Handle it carefully knowing that it looks just like cold glassware (A)</p> Signup and view all the answers

    According to the information, what does chemical toxicity depend on?

    <p>Concentration (dose), frequency, duration, and route of exposure. (B)</p> Signup and view all the answers

    What is the primary difference between acute and chronic exposure to a substance?

    <p>Acute exposure is short-term and severe, while chronic exposure is long-term and may have less obvious beginning symptoms. (D)</p> Signup and view all the answers

    What does the acronym 'SIN' stand for in emergency situations?

    <p>Safety, Isolation, Notification. (C)</p> Signup and view all the answers

    The PASS method is associated with which fire safety procedure?

    <p>Using a fire extinguisher. (D)</p> Signup and view all the answers

    What term describes the source of danger, such as a chemical or hot surface?

    <p>Hazard (A)</p> Signup and view all the answers

    What action should be taken first if a classmate's clothing catches fire?

    <p>Smother the flames using a blanket or rug. (D)</p> Signup and view all the answers

    Which of the following is a physical effect associated with exposure to cryogens?

    <p>Frostbite (B)</p> Signup and view all the answers

    What is the relationship between dosage and toxicity?

    <p>The right dose is what determines if a substance is safe or toxic. (D)</p> Signup and view all the answers

    Repetitive motion, awkward posture, and improper lifting are categorized under which type of hazards?

    <p>Ergonomic hazards (C)</p> Signup and view all the answers

    What should be done with excess chemicals after weighing?

    <p>Discard the excess according to the established protocols. (A)</p> Signup and view all the answers

    Which of these is an example of Ionizing radiation?

    <p>X-rays (D)</p> Signup and view all the answers

    Which scenario is an example of chronic exposure, based on the information provided?

    <p>A laboratory technician is exposed to low levels of dust over several months. (C)</p> Signup and view all the answers

    Why should materials never be placed temporarily on the floor in the lab?

    <p>To prevent accidental spills or trips. (C)</p> Signup and view all the answers

    Which of the following terms describes substances that can ignite spontaneously?

    <p>Pyrophoric (C)</p> Signup and view all the answers

    What is one of the emergency situations listed that could occur?

    <p>A power outage. (B)</p> Signup and view all the answers

    When should laboratory equipment be inspected for damage?

    <p>Prior to each use, every time. (A)</p> Signup and view all the answers

    A chemical that results in a physical burn or an explosion belongs to which hazard category?

    <p>Physical Hazard (A)</p> Signup and view all the answers

    What personal hygiene practice should be followed when handling chemicals in the laboratory?

    <p>Wash hands after removing gloves and before exiting. (A)</p> Signup and view all the answers

    Which term is used to describe chemicals that upon entry to the body can cause allergic reactions?

    <p>Sensitizer (C)</p> Signup and view all the answers

    What is the primary reason for holding chemical containers away from the body when transferring?

    <p>To minimize the risk of chemical exposure. (A)</p> Signup and view all the answers

    What route of chemical exposure is indicated by breathing in fumes from volatile solvents?

    <p>Inhalation (A)</p> Signup and view all the answers

    Which procedure should be followed when disposing of broken glassware?

    <p>Place it immediately in designated containers. (A)</p> Signup and view all the answers

    Besides fire extinguishers, fire blankets, alarm systems and first aid kits, what other emergency equipment should a laboratory worker be familiar with?

    <p>Eye washes and safety showers. (D)</p> Signup and view all the answers

    What is the appropriate way to handle a solid reagent when removing it from a container?

    <p>Use a spatula. (A)</p> Signup and view all the answers

    When should you NOT fight a fire?

    <p>When the fire is spreading beyond its origin. (B)</p> Signup and view all the answers

    What should you immediately do if a chemical splashes into your eyes?

    <p>Use the safety eyewash immediately and thoroughly. (D)</p> Signup and view all the answers

    What is the recommended way to smell a substance in the lab?

    <p>Quickly waft the air above the substance towards your nose. (B)</p> Signup and view all the answers

    What is the first action when cleaning a chemical spill?

    <p>Absorb the excess spill and surround the area with absorbent material. (D)</p> Signup and view all the answers

    If you are fighting a fire and the extinguisher runs out of agent, what should you do?

    <p>Leave the area immediately. (D)</p> Signup and view all the answers

    Why is it important to point the end of a test tube away from yourself and others?

    <p>To avoid any accidental projections of the contents. (B)</p> Signup and view all the answers

    What should you do with clothes contaminated by a chemical spill?

    <p>Launder them separately or discard according to MSDS. (D)</p> Signup and view all the answers

    When treating chemical burns, what is generally the first step?

    <p>Wash the affected area with cold water for 10 minutes. (B)</p> Signup and view all the answers

    Which term describes a substance that causes a rapid toxic effect after exposure?

    <p>Acute (C)</p> Signup and view all the answers

    What is a material that causes chemical burns or eats away at equipment is known as?

    <p>Caustic (A)</p> Signup and view all the answers

    Which of the following describes a material that forms explosive peroxides when exposed to air?

    <p>Peroxide former (D)</p> Signup and view all the answers

    What is the lowest temperature at which a vapor given off by a material will ignite?

    <p>Flash point (D)</p> Signup and view all the answers

    According to the NFPA 704 labeling system, what does a '4' indicate for flammability?

    <p>Below 73°F (D)</p> Signup and view all the answers

    What is the primary purpose of secondary container labeling?

    <p>To display the chemical identity and associated hazards (C)</p> Signup and view all the answers

    What does the 'OX' symbol indicate on a chemical label?

    <p>Oxidizing agent (A)</p> Signup and view all the answers

    What does the term 'Lacrymator' refer to?

    <p>A material that causes irritation to eyes, skin, or respiratory system. (B)</p> Signup and view all the answers

    Study Notes

    Chemical Laboratory Safety Orientation

    • Chemical laboratory work involves hazardous materials.
    • Accident prevention is a shared responsibility among all lab personnel.
    • Safe practices are essential, not just preferred.

    Fundamentals of Chemical Laboratory Safety

    • Every day chemicals used in laboratories can be hazardous.
    • Preventing accidents is the responsibility of every lab member.
    • Safe practices are crucial in laboratories.

    Personal Protection Equipment (PPE)

    • Wear appropriate PPE
      • Chemical splash goggles
      • Chemical-resistant apron
      • Shoes that fully cover the whole foot
      • Safety gloves
      • Face mask (not surgical)

    Laboratory Protocol

    • Do not engage in unauthorized activities, including practical jokes or unsanctioned experiments.
    • Avoid unnecessary running or conversations in the laboratory.
    • Prohibit using personal audio or video equipment in the laboratory.
    • Do not sit on lab work areas or sinks
    • Do not work unsupervised.

    General Work Procedures

    • Exercise caution when handling hot glass and apparatus.

    • Hot glassware looks just like cold glassware, so always treat it carefully.

    • Avoid pipetting chemicals by mouth. Always use pipetting aids or suction bulbs.

    • Report any spills, incidents, or injuries to the teacher immediately.

    • Ensure there are no flammable solvents in the vicinity during flame tests.

    • Turn off all heating equipment, gas valves, burners, and water faucets when not in use.

    • Store lab coats, bags, and other personal items in designated areas; not on lab benches or aisles.

    Housekeeping

    • Never place materials, particularly chemicals, on the floor, even temporarily.
    • Maintain well-organized workspaces and storage areas free of broken glass, surplus chemicals, and unnecessary items.
    • Never obstruct access to exits or emergency equipment.
    • Verify all equipment for damage prior to its use.

    Hygiene Practices

    • Avoid touching your face, eyes, mouth, and body with chemicals.
    • Foods and drinks are not permitted within the laboratory area.
    • Wash your hands carefully after removing gloves and before leaving the laboratory.

    Emergency Procedures

    • Know the location of all exits and follow the emergency plan.
    • Know how to operate fire extinguishers, fire blankets, alarm systems with pull stations, eyewashes, safety showers, and first aid kits.

    Chemical Handling Procedures

    • Verify the label before using any chemical to ensure it's the correct substance.
    • Utilize spatulas to remove solid reagents from containers.
    • Hold containers away from your body when transferring liquids or chemicals.
    • Measure only the required amount of chemical and do not return any excess to its original container.

    Waste Disposal

    • Do not discard chemical waste down the laboratory sinks.
    • Dispose of chemical wastes in properly labelled containers.
    • Properly dispose of broken glassware and sharp objects (e.g., syringe needles) in designated containers.
    • Place waste paper products that aren't chemical laden in the appropriate trash receptacles.

    Safety Considerations

    • Hazards are danger sources (like chemicals, electricity, hot surfaces, etc.).
    • Risk is the likelihood of a hazard occurring, considering the length of exposure.
    • Consequence is the outcome or impact of exposure to a hazard.

    Physical Hazards

    • Heat and fire cause thermal burns.
    • Cryogens cause frostbite.
    • Ultraviolet exposure causes sunburn.
    • Explosions (chemical reactions, compressed gases) lead to projectile injuries, impacts/bruises, or injections.
    • Noise leads to hearing damage.

    Ergonomic Hazards

    • Repetitive motions, heavy lifting, workspaces design (computers, instruments), and awkward postures cause discomfort.

    Bio Hazards

    • Bloodborne pathogens (AIDS, HIV, hepatitis) are found in clinical chemistry labs and labs involving genetic engineering/cloning, animal work, and zoology.
    • Zonotic diseases originate from animals.

    Radiation Hazards

    • Ionizing radiation (alpha, beta, gamma, X-rays, neutrons) and radioactive isotopes (tritium H-3, carbon C-14, sulfur S-35, phosphorus P-32/33, iodine I-135) are dangerous.

    Fire and Explosion Hazards

    • Flammable solvents, pyrophoric materials, and spontaneous combustion pose fire hazards.

    Special Chemical Substances

    • Controlled substances (drugs, psychotropics, hallucinogens, heroin).
    • Chemical warfare agents (nerve gas, phosgene, riot control agents) are particularly dangerous.

    Chemical Exposure

    • Chemical exposures occur via physical and health routes (air, water, food, soil, products).
    • Toxicities depend on concentration, frequency, duration, and exposure route.
    • Physical hazards include chemical burns (acids/caustics), physical burns (flammables/combustibles), explosions (chemical reactions/compressed gases), and asphyxiation (lack of oxygen).
    • Health hazards include irritants, allergens/sensitizers, reproductive/developmental toxins, carcinogens, target organ effects, and chemical asphyxiants.

    Routes of Exposure

    • Inhalation is a significant mode of chemical exposure, especially when working in the breathing zone. Others include absorption, ingestion, and injection.

    How to Avoid Accidents in the Lab

    • Point test tubes away from yourself and others.
    • Look at test tube contents from the side.
    • Tie back loose hair to prevent contact with lab materials.
    • Never taste substances without a direct instruction; instead, fan your hand by the substance to direct the odor/smell to your nose.

    Chemicals on Skin, Clothing, and Eyes

    • Flush spills immediately with a safety shower.
    • Launder contaminated clothes separately.
    • Rinse any eyes exposed immediately with the safety eyewash.
    • For chemical burns, wash with cold water for 10 minutes.
    • For organic burns, use soap to emulsify the substance, then wash with water

    Spill Cleanup

    • Immediately and efficiently clean up spills.
    • Absorb excess liquid using absorbent materials.
    • Wear appropriate PPE.
    • Use tongs/forceps to pick up broken glassware.
    • Clean from outside to inside the spill area.
    • Avoid dry sweeping.

    Material Safety Data Terminology

    • Poison: Material with toxic effects.
    • Toxin: Poisonous substance.
    • Acute: Rapid onset of toxic effects.
    • Chronic: Toxic effects develop over time.
    • Cumulative: Toxins accumulate in the body.
    • Carcinogen: Cancer-causing material.
    • Mutagen: Material that causes genetic mutations.
    • Teratogen: Material that causes birth defects.
    • Caustic: Substance that burns or damages tissue.
    • Explosive: Substance that can explode.
    • Oxidizer: Substance that can cause other materials to catch fire.
    • Peroxide former: Substance that forms peroxides when exposed to air/water
    • Flash point: Lowest temperature at which a liquid vaporizes and ignites.
    • Corrosive: Damages or destroys materials.
    • Lacrimator: Produces eye irritation.
    • Flammable: Substance that ignites easily.
    • Inflammable: Same as flammable
    • Combustible: Substance that catches fire.

    Chemical Labeling

    • Primary container labels include manufacturer, hazards, and identity.
    • Secondary container labels display hazards (descriptions, icons, etc.).

    Labeling: NFPA and DOT

    • Understanding the NFPA 704 and DOT safety symbols is critical for recognizing chemical hazards and risks.

    Fire Safety

    • Preventing fires is the best approach.
    • Correctly use fire extinguishers, following the PASS method (Pull, Aim, Squeeze, Sweep).
    • Know when NOT to tackle a fire (e.g., too large, spreading rapidly, blocking escape routes, inadequate PPE).
    • Immediately leave the area in such situations; contact relevant authorities.
    • If a person's clothes catch fire, have them STOP, DROP, and ROLL.
    • Use a blanket or rug to smother flames, if applicable.

    Emergency Situations and Actions

    • Be ready for building alarms, earthquakes, and medical emergencies.
    • If a criminal act occurs, move to safety and contact relevant authorities.
    • Handle chemical or radioactive spills/contamination appropriately and contact relevant authorities.
    • In all cases of emergency, follow safety protocols: ensure you are safe first, then assist others.

    How to Handle Laboratory Accidents

    • Dealing with incidents is just as critical as avoiding them.

    Chemical Toxicity

    • Understand that all substances are potentially harmful; the dosage determines harm.
    • Recognize the distinction between acute (rapid onset) and chronic (prolonged) toxicity.

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