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Questions and Answers
What is the primary reason for adhering to chemical safety protocols in a laboratory?
What is the primary reason for adhering to chemical safety protocols in a laboratory?
Which of the following is considered appropriate Personal Protective Equipment (PPE) to wear in a chemical laboratory?
Which of the following is considered appropriate Personal Protective Equipment (PPE) to wear in a chemical laboratory?
Which of the following activities is NOT allowed in a chemical lab?
Which of the following activities is NOT allowed in a chemical lab?
What is the correct procedure for using a pipette in the laboratory?
What is the correct procedure for using a pipette in the laboratory?
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What should you do immediately after an accident or injury occurs in the lab?
What should you do immediately after an accident or injury occurs in the lab?
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What should be done with coats, bags, and other personal items in the laboratory?
What should be done with coats, bags, and other personal items in the laboratory?
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When should heating apparatus, gas valves, and water faucets be turned off?
When should heating apparatus, gas valves, and water faucets be turned off?
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What is one important precaution to take when working with hot glassware?
What is one important precaution to take when working with hot glassware?
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According to the information, what does chemical toxicity depend on?
According to the information, what does chemical toxicity depend on?
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What is the primary difference between acute and chronic exposure to a substance?
What is the primary difference between acute and chronic exposure to a substance?
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What does the acronym 'SIN' stand for in emergency situations?
What does the acronym 'SIN' stand for in emergency situations?
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The PASS method is associated with which fire safety procedure?
The PASS method is associated with which fire safety procedure?
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What term describes the source of danger, such as a chemical or hot surface?
What term describes the source of danger, such as a chemical or hot surface?
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What action should be taken first if a classmate's clothing catches fire?
What action should be taken first if a classmate's clothing catches fire?
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Which of the following is a physical effect associated with exposure to cryogens?
Which of the following is a physical effect associated with exposure to cryogens?
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What is the relationship between dosage and toxicity?
What is the relationship between dosage and toxicity?
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Repetitive motion, awkward posture, and improper lifting are categorized under which type of hazards?
Repetitive motion, awkward posture, and improper lifting are categorized under which type of hazards?
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What should be done with excess chemicals after weighing?
What should be done with excess chemicals after weighing?
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Which of these is an example of Ionizing radiation?
Which of these is an example of Ionizing radiation?
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Which scenario is an example of chronic exposure, based on the information provided?
Which scenario is an example of chronic exposure, based on the information provided?
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Why should materials never be placed temporarily on the floor in the lab?
Why should materials never be placed temporarily on the floor in the lab?
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Which of the following terms describes substances that can ignite spontaneously?
Which of the following terms describes substances that can ignite spontaneously?
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What is one of the emergency situations listed that could occur?
What is one of the emergency situations listed that could occur?
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When should laboratory equipment be inspected for damage?
When should laboratory equipment be inspected for damage?
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A chemical that results in a physical burn or an explosion belongs to which hazard category?
A chemical that results in a physical burn or an explosion belongs to which hazard category?
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What personal hygiene practice should be followed when handling chemicals in the laboratory?
What personal hygiene practice should be followed when handling chemicals in the laboratory?
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Which term is used to describe chemicals that upon entry to the body can cause allergic reactions?
Which term is used to describe chemicals that upon entry to the body can cause allergic reactions?
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What is the primary reason for holding chemical containers away from the body when transferring?
What is the primary reason for holding chemical containers away from the body when transferring?
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What route of chemical exposure is indicated by breathing in fumes from volatile solvents?
What route of chemical exposure is indicated by breathing in fumes from volatile solvents?
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Which procedure should be followed when disposing of broken glassware?
Which procedure should be followed when disposing of broken glassware?
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Besides fire extinguishers, fire blankets, alarm systems and first aid kits, what other emergency equipment should a laboratory worker be familiar with?
Besides fire extinguishers, fire blankets, alarm systems and first aid kits, what other emergency equipment should a laboratory worker be familiar with?
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What is the appropriate way to handle a solid reagent when removing it from a container?
What is the appropriate way to handle a solid reagent when removing it from a container?
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When should you NOT fight a fire?
When should you NOT fight a fire?
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What should you immediately do if a chemical splashes into your eyes?
What should you immediately do if a chemical splashes into your eyes?
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What is the recommended way to smell a substance in the lab?
What is the recommended way to smell a substance in the lab?
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What is the first action when cleaning a chemical spill?
What is the first action when cleaning a chemical spill?
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If you are fighting a fire and the extinguisher runs out of agent, what should you do?
If you are fighting a fire and the extinguisher runs out of agent, what should you do?
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Why is it important to point the end of a test tube away from yourself and others?
Why is it important to point the end of a test tube away from yourself and others?
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What should you do with clothes contaminated by a chemical spill?
What should you do with clothes contaminated by a chemical spill?
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When treating chemical burns, what is generally the first step?
When treating chemical burns, what is generally the first step?
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Which term describes a substance that causes a rapid toxic effect after exposure?
Which term describes a substance that causes a rapid toxic effect after exposure?
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What is a material that causes chemical burns or eats away at equipment is known as?
What is a material that causes chemical burns or eats away at equipment is known as?
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Which of the following describes a material that forms explosive peroxides when exposed to air?
Which of the following describes a material that forms explosive peroxides when exposed to air?
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What is the lowest temperature at which a vapor given off by a material will ignite?
What is the lowest temperature at which a vapor given off by a material will ignite?
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According to the NFPA 704 labeling system, what does a '4' indicate for flammability?
According to the NFPA 704 labeling system, what does a '4' indicate for flammability?
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What is the primary purpose of secondary container labeling?
What is the primary purpose of secondary container labeling?
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What does the 'OX' symbol indicate on a chemical label?
What does the 'OX' symbol indicate on a chemical label?
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What does the term 'Lacrymator' refer to?
What does the term 'Lacrymator' refer to?
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Study Notes
Chemical Laboratory Safety Orientation
- Chemical laboratory work involves hazardous materials.
- Accident prevention is a shared responsibility among all lab personnel.
- Safe practices are essential, not just preferred.
Fundamentals of Chemical Laboratory Safety
- Every day chemicals used in laboratories can be hazardous.
- Preventing accidents is the responsibility of every lab member.
- Safe practices are crucial in laboratories.
Personal Protection Equipment (PPE)
- Wear appropriate PPE
- Chemical splash goggles
- Chemical-resistant apron
- Shoes that fully cover the whole foot
- Safety gloves
- Face mask (not surgical)
Laboratory Protocol
- Do not engage in unauthorized activities, including practical jokes or unsanctioned experiments.
- Avoid unnecessary running or conversations in the laboratory.
- Prohibit using personal audio or video equipment in the laboratory.
- Do not sit on lab work areas or sinks
- Do not work unsupervised.
General Work Procedures
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Exercise caution when handling hot glass and apparatus.
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Hot glassware looks just like cold glassware, so always treat it carefully.
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Avoid pipetting chemicals by mouth. Always use pipetting aids or suction bulbs.
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Report any spills, incidents, or injuries to the teacher immediately.
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Ensure there are no flammable solvents in the vicinity during flame tests.
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Turn off all heating equipment, gas valves, burners, and water faucets when not in use.
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Store lab coats, bags, and other personal items in designated areas; not on lab benches or aisles.
Housekeeping
- Never place materials, particularly chemicals, on the floor, even temporarily.
- Maintain well-organized workspaces and storage areas free of broken glass, surplus chemicals, and unnecessary items.
- Never obstruct access to exits or emergency equipment.
- Verify all equipment for damage prior to its use.
Hygiene Practices
- Avoid touching your face, eyes, mouth, and body with chemicals.
- Foods and drinks are not permitted within the laboratory area.
- Wash your hands carefully after removing gloves and before leaving the laboratory.
Emergency Procedures
- Know the location of all exits and follow the emergency plan.
- Know how to operate fire extinguishers, fire blankets, alarm systems with pull stations, eyewashes, safety showers, and first aid kits.
Chemical Handling Procedures
- Verify the label before using any chemical to ensure it's the correct substance.
- Utilize spatulas to remove solid reagents from containers.
- Hold containers away from your body when transferring liquids or chemicals.
- Measure only the required amount of chemical and do not return any excess to its original container.
Waste Disposal
- Do not discard chemical waste down the laboratory sinks.
- Dispose of chemical wastes in properly labelled containers.
- Properly dispose of broken glassware and sharp objects (e.g., syringe needles) in designated containers.
- Place waste paper products that aren't chemical laden in the appropriate trash receptacles.
Safety Considerations
- Hazards are danger sources (like chemicals, electricity, hot surfaces, etc.).
- Risk is the likelihood of a hazard occurring, considering the length of exposure.
- Consequence is the outcome or impact of exposure to a hazard.
Physical Hazards
- Heat and fire cause thermal burns.
- Cryogens cause frostbite.
- Ultraviolet exposure causes sunburn.
- Explosions (chemical reactions, compressed gases) lead to projectile injuries, impacts/bruises, or injections.
- Noise leads to hearing damage.
Ergonomic Hazards
- Repetitive motions, heavy lifting, workspaces design (computers, instruments), and awkward postures cause discomfort.
Bio Hazards
- Bloodborne pathogens (AIDS, HIV, hepatitis) are found in clinical chemistry labs and labs involving genetic engineering/cloning, animal work, and zoology.
- Zonotic diseases originate from animals.
Radiation Hazards
- Ionizing radiation (alpha, beta, gamma, X-rays, neutrons) and radioactive isotopes (tritium H-3, carbon C-14, sulfur S-35, phosphorus P-32/33, iodine I-135) are dangerous.
Fire and Explosion Hazards
- Flammable solvents, pyrophoric materials, and spontaneous combustion pose fire hazards.
Special Chemical Substances
- Controlled substances (drugs, psychotropics, hallucinogens, heroin).
- Chemical warfare agents (nerve gas, phosgene, riot control agents) are particularly dangerous.
Chemical Exposure
- Chemical exposures occur via physical and health routes (air, water, food, soil, products).
- Toxicities depend on concentration, frequency, duration, and exposure route.
- Physical hazards include chemical burns (acids/caustics), physical burns (flammables/combustibles), explosions (chemical reactions/compressed gases), and asphyxiation (lack of oxygen).
- Health hazards include irritants, allergens/sensitizers, reproductive/developmental toxins, carcinogens, target organ effects, and chemical asphyxiants.
Routes of Exposure
- Inhalation is a significant mode of chemical exposure, especially when working in the breathing zone. Others include absorption, ingestion, and injection.
How to Avoid Accidents in the Lab
- Point test tubes away from yourself and others.
- Look at test tube contents from the side.
- Tie back loose hair to prevent contact with lab materials.
- Never taste substances without a direct instruction; instead, fan your hand by the substance to direct the odor/smell to your nose.
Chemicals on Skin, Clothing, and Eyes
- Flush spills immediately with a safety shower.
- Launder contaminated clothes separately.
- Rinse any eyes exposed immediately with the safety eyewash.
- For chemical burns, wash with cold water for 10 minutes.
- For organic burns, use soap to emulsify the substance, then wash with water
Spill Cleanup
- Immediately and efficiently clean up spills.
- Absorb excess liquid using absorbent materials.
- Wear appropriate PPE.
- Use tongs/forceps to pick up broken glassware.
- Clean from outside to inside the spill area.
- Avoid dry sweeping.
Material Safety Data Terminology
- Poison: Material with toxic effects.
- Toxin: Poisonous substance.
- Acute: Rapid onset of toxic effects.
- Chronic: Toxic effects develop over time.
- Cumulative: Toxins accumulate in the body.
- Carcinogen: Cancer-causing material.
- Mutagen: Material that causes genetic mutations.
- Teratogen: Material that causes birth defects.
- Caustic: Substance that burns or damages tissue.
- Explosive: Substance that can explode.
- Oxidizer: Substance that can cause other materials to catch fire.
- Peroxide former: Substance that forms peroxides when exposed to air/water
- Flash point: Lowest temperature at which a liquid vaporizes and ignites.
- Corrosive: Damages or destroys materials.
- Lacrimator: Produces eye irritation.
- Flammable: Substance that ignites easily.
- Inflammable: Same as flammable
- Combustible: Substance that catches fire.
Chemical Labeling
- Primary container labels include manufacturer, hazards, and identity.
- Secondary container labels display hazards (descriptions, icons, etc.).
Labeling: NFPA and DOT
- Understanding the NFPA 704 and DOT safety symbols is critical for recognizing chemical hazards and risks.
Fire Safety
- Preventing fires is the best approach.
- Correctly use fire extinguishers, following the PASS method (Pull, Aim, Squeeze, Sweep).
- Know when NOT to tackle a fire (e.g., too large, spreading rapidly, blocking escape routes, inadequate PPE).
- Immediately leave the area in such situations; contact relevant authorities.
- If a person's clothes catch fire, have them STOP, DROP, and ROLL.
- Use a blanket or rug to smother flames, if applicable.
Emergency Situations and Actions
- Be ready for building alarms, earthquakes, and medical emergencies.
- If a criminal act occurs, move to safety and contact relevant authorities.
- Handle chemical or radioactive spills/contamination appropriately and contact relevant authorities.
- In all cases of emergency, follow safety protocols: ensure you are safe first, then assist others.
How to Handle Laboratory Accidents
- Dealing with incidents is just as critical as avoiding them.
Chemical Toxicity
- Understand that all substances are potentially harmful; the dosage determines harm.
- Recognize the distinction between acute (rapid onset) and chronic (prolonged) toxicity.
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Description
Test your knowledge on chemical safety protocols and personal protective equipment in laboratory settings. This quiz will cover crucial procedures, emergency actions, and safety precautions necessary for maintaining a safe laboratory environment. Prepare to ensure your safety and that of others while working with hazardous materials.