Cellular Organelles Overview

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Questions and Answers

What is the primary function of the nucleus in a cell?

  • To serve as a structural framework for the cell
  • To control cell activities by directing protein synthesis (correct)
  • To store waste products
  • To produce energy through cellular respiration

What is the role of the nucleolus within the nucleus?

  • To synthesize lipids
  • To regulate the passage of materials
  • To produce messenger RNA
  • To synthesize ribosomal RNA (correct)

Which cellular organelle is responsible for converting hydrogen peroxide to water?

  • Lysosomes
  • Peroxisomes (correct)
  • Mitochondria
  • Golgi apparatus

What is the structure and function of the centrosome in animal cells?

<p>A microtubule-organizing center containing centrioles (A)</p> Signup and view all the answers

What are the three kinds of fibers that make up the cytoskeleton?

<p>Microfilaments, intermediate filaments, and microtubules (B)</p> Signup and view all the answers

What provides structural support and motility to the cell?

<p>Cytoskeleton (D)</p> Signup and view all the answers

Which of the following statements about microfilaments is true?

<p>They contain the protein myosin in addition to actin (B)</p> Signup and view all the answers

What feature of the nuclear envelope allows substances to enter and exit the nucleus?

<p>Pores that allow material flow (D)</p> Signup and view all the answers

What function do intermediate filaments serve in muscle cells?

<p>Reinforce cell shape and anchor organelles (A)</p> Signup and view all the answers

Which of the following structures is composed of tubulin?

<p>Microtubules (C)</p> Signup and view all the answers

What is the primary role of the extracellular matrix (ECM) in animal cells?

<p>To support and regulate cell structure (B)</p> Signup and view all the answers

How do cilia and flagella achieve movement?

<p>Through the bending of motor proteins called dynein (A)</p> Signup and view all the answers

Which of the following best describes the 9 + 2 pattern found in cilia and flagella?

<p>Nine pairs of microtubule doublets surrounding a central pair (B)</p> Signup and view all the answers

In muscle cell contraction, what role do myosin filaments play?

<p>Generate force by interacting with actin filaments (D)</p> Signup and view all the answers

What is the function of integrins in the extracellular matrix?

<p>To connect the ECM to the plasma membrane (C)</p> Signup and view all the answers

What characteristic distinguishes microfilaments from other cytoskeletal elements?

<p>They are composed of actin subunits and aid in muscle contraction. (B)</p> Signup and view all the answers

What is the primary function of tight junctions in epithelial cells?

<p>Preventing leakage of extracellular fluid (D)</p> Signup and view all the answers

Which type of cell junction is primarily responsible for connecting adjacent cells and ensuring structural cohesion in tissues under mechanical stress?

<p>Desmosomes (A)</p> Signup and view all the answers

During cell fractionation, which component is likely to be rich in ribosomes after centrifugation?

<p>Pellet rich in microsomes (C)</p> Signup and view all the answers

What role do gap junctions play in animal tissues?

<p>Mediating rapid exchange of small molecules (A)</p> Signup and view all the answers

In cell fractionation, what is the purpose of homogenization?

<p>To mix the cellular components evenly (D)</p> Signup and view all the answers

Which of the following statements about anchoring junctions is true?

<p>They connect to components of the extracellular matrix. (C)</p> Signup and view all the answers

Which centrifuge setting would be expected to yield a pellet rich in nuclei?

<p>1,000 g for 10 min (D)</p> Signup and view all the answers

What is the primary function of the cytoskeleton in maintaining cell structure?

<p>Providing mechanical support (C)</p> Signup and view all the answers

What distinguishes the gap junctions from other types of cell junctions?

<p>They allow for the exchange of ions and small molecules. (C)</p> Signup and view all the answers

Which fractional step in centrifugation is typically associated with isolating mitochondria?

<p>80,000 g for 60 min (C)</p> Signup and view all the answers

Flashcards

What is the nucleus?

The nucleus is the cell's control center, containing most of the cell's DNA and directing protein synthesis via making mRNA. DNA is associated with proteins in structures called chromosomes. Each chromosome is composed of a single DNA molecule associated with proteins.

What is the nuclear envelope?

The nuclear envelope is a double membrane with pores allowing material to flow in and out of the nucleus. It's attached to the endoplasmic reticulum.

What is the nucleolus?

The nucleolus is a prominent structure in the nucleus responsible for ribosomal RNA (rRNA) synthesis.

What are peroxisomes?

Peroxisomes are single-membrane bound compartments that produce hydrogen peroxide and convert it to water. They perform reactions with diverse functions.

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What is the cytoskeleton?

The cytoskeleton is a network of protein fibers providing structural support and enabling motility. It interacts with motor proteins for cellular regulation and movement

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What are microfilaments?

Microfilaments (actin filaments) are involved in cell shape and motility. They contain the protein myosin for movement.

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What are microtubules?

Microtubules are hollow tubes, larger than microfilaments, involved in cell shape, movement, and vesicle transport. They are composed of tubulin protein

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What are centrosomes and centrioles?

Centrosomes are microtubule-organizing centers in many cells, often containing a pair of centrioles in animal cells which are involved in cell division

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Actin filaments in muscle cells

Actin filaments are abundant in muscle cells and arranged in parallel lines. Their primary function is in muscle contraction.

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Function of intermediate filaments

Intermediate filaments provide structural support to cells, helping maintain their shape and anchoring organelles in place.

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Function of microtubules

Microtubules, made of tubulin protein, provide rigidity to cells and act as tracks for moving organelles within the cell.

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How do muscles contract?

Muscle contraction involves the sliding of actin and myosin filaments past each other, powered by myosin motor proteins.

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What are cilia and flagella?

Cilia and flagella are hair-like projections found on some cells. They are composed of microtubules and aid in movement by bending.

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Microtubule arrangement in cilia and flagella

Cilia and flagella have a characteristic 9 + 2 arrangement of microtubules, with a central pair surrounded by nine microtubule doublets.

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What is the extracellular matrix (ECM)?

The extracellular matrix (ECM) is a network of molecules outside animal cells, providing support, holding cells together, and acting as a barrier for the plasma membrane.

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Function of integrins

Integrins are proteins that connect the ECM to the cytoskeleton, providing a link between the cell's internal structure and its surroundings.

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Cell junctions

Specialized connections between adjacent cells that allow for communication, interaction, and adhesion. They create a functional unit by coordinating cellular activities.

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Tight junctions

Prevent leakage of extracellular fluid across a layer of epithelial cells. They act like tight seals.

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Anchoring junctions

Anchored to each other and to components of the extracellular matrix. They provide structural support and withstand mechanical stress.

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Gap junctions

Channels that physically connect adjacent cells, allowing the rapid exchange of small molecules. Important for communication and coordination.

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Cell fractionation

A process that separates cells into their major organelles. Used to study the functions of different cellular components.

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Homogenate

A mixture of broken-down cells with organelles released into the solution.

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Differential centrifugation

A method that uses a centrifuge to separate organelles based on their size and density. It uses different speeds and durations of centrifugation.

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Supernatant

The liquid portion that floats on top of the pellet after centrifugation. Contains smaller organelles or components.

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Pellet

The solid mass of material that settles at the bottom of the centrifuge tube.

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Glycoprotein

A complex of proteins and carbohydrates found on the exterior surface of the plasma membrane. They help in cell recognition and adhesion.

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Study Notes

Cellular Organelles

  • Cells contain various organelles, including the nucleus, peroxisomes, and the endoplasmic reticulum.

Nucleus

  • The nucleus acts as the cell's control center.
  • It contains most of the cell's DNA.
  • DNA directs protein synthesis through messenger RNA (mRNA).
  • DNA is associated with proteins in structures known as chromosomes.
  • Each chromosome is made up of a single DNA molecule.
  • The nucleus has a double membrane called the nuclear envelope.
  • The nuclear envelope contains pores that allow material to enter and exit the nucleus.
  • It is connected to the endoplasmic reticulum.
  • The nucleolus is inside the nucleus and is responsible for synthesizing ribosomal RNA (rRNA).

Peroxisomes

  • Peroxisomes are specialized metabolic compartments.
  • They are enclosed by a single membrane.
  • They produce hydrogen peroxide.
  • They convert hydrogen peroxide into water.
  • Peroxisomes carry out various reactions.

Cell Fractionation

  • Cell fractionation separates organelles from one another.
  • Centrifuges are used to separate cellular components.
  • This method allows scientists to determine the function of certain organelles.
  • Biochemistry and cytology aid in correlating cell function with structure.

Centrosomes and Centrioles

  • Microtubules grow out of centrosomes, which are located near the nucleus.
  • Centrioles, consisting of nine triplets of microtubules arranged in a ring, are found in animal cells within the centrosome.

Cytoskeleton

  • The cytoskeleton is a network of protein fibers that help organize the cell's structure and activities.
  • The cytoskeleton includes microfilaments (actin filaments), intermediate filaments, and microtubules.
  • Microfilaments maintain cell shape and are involved in movement.
  • Actin filaments and myosin are involved in muscle cell contraction.
  • Intermediate filaments maintain cell shape.
  • Microtubules provide cell rigidity and serve as tracks for organelle movement.

Cilia and Flagella

  • Cilia and flagella are appendages made of microtubules embedded in the plasma membrane.
  • They help protists move and they have different specialized functions in multicellular organisms.
  • Cilia in the lungs help sweep mucus.
  • Sperm cells in animals use flagella for movement.
  • Cilia and flagella have a 9 + 2 pattern of microtubule arrangement.
  • Dynein proteins are bending motor proteins in cilia and flagella.

Extracellular Matrix

  • Animal cells secrete an extracellular matrix (ECM), which helps hold cells together in tissues and supports the plasma membrane.
  • The ECM is connected to the cell through glycoproteins and membrane proteins called integrins.
  • Integrins connect to the microfilaments of the cytoskeleton, providing structural support.

Cell Junctions

  • Specialized junctions between cells allow for communication and adhesion.
  • Tight junctions prevent fluid leakage.
  • Anchoring junctions link cells and the extracellular matrix.
  • Gap junctions allow for the rapid exchange of small molecules.

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