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Questions and Answers
What is the primary source of metabolic energy for all cells?
What is the primary source of metabolic energy for all cells?
What process involves breaking down glucose to pyruvate, yielding ATP and NADH?
What process involves breaking down glucose to pyruvate, yielding ATP and NADH?
Which law of thermodynamics determines the direction of chemical reactions?
Which law of thermodynamics determines the direction of chemical reactions?
What is responsible for the synthesis of major cell constituents including carbohydrates, lipids, proteins, and nucleic acids?
What is responsible for the synthesis of major cell constituents including carbohydrates, lipids, proteins, and nucleic acids?
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Where does fatty acid synthesis primarily occur?
Where does fatty acid synthesis primarily occur?
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What is the precursor for both purine and pyrimidine nucleotide synthesis?
What is the precursor for both purine and pyrimidine nucleotide synthesis?
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What process converts atmospheric nitrogen (N2) into ammonia (NH3)?
What process converts atmospheric nitrogen (N2) into ammonia (NH3)?
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What is the end product of glycolysis?
What is the end product of glycolysis?
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What drives the formation of peptide bonds during protein synthesis?
What drives the formation of peptide bonds during protein synthesis?
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How do antimetabolites like 6-mercaptopurine work?
How do antimetabolites like 6-mercaptopurine work?
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What does Rubisco catalyze in the Calvin cycle?
What does Rubisco catalyze in the Calvin cycle?
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From which citric acid cycle intermediate is glutamate directly synthesized?
From which citric acid cycle intermediate is glutamate directly synthesized?
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What is the final electron acceptor in the mitochondrial electron transport chain?
What is the final electron acceptor in the mitochondrial electron transport chain?
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In what form is glucose stored in animal cells?
In what form is glucose stored in animal cells?
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Where does fatty acid synthesis primarily occur in eukaryotic cells?
Where does fatty acid synthesis primarily occur in eukaryotic cells?
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What acts as the final electron acceptor in the light reactions of photosynthesis?
What acts as the final electron acceptor in the light reactions of photosynthesis?
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What is the activated form of glucose used in the synthesis of polysaccharides?
What is the activated form of glucose used in the synthesis of polysaccharides?
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What is the major product of fatty acid synthesis in eukaryotic cells?
What is the major product of fatty acid synthesis in eukaryotic cells?
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What is the starting point for nucleotide biosynthesis (both pyrimidine and purine)?
What is the starting point for nucleotide biosynthesis (both pyrimidine and purine)?
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What is the primary CO$_2$ acceptor in the Calvin cycle, facilitating CO$_2$ fixation?
What is the primary CO$_2$ acceptor in the Calvin cycle, facilitating CO$_2$ fixation?
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How do antimetabolites like 6-mercaptopurine work?
How do antimetabolites like 6-mercaptopurine work?
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What is the primary source of carbon skeletons for amino acids?
What is the primary source of carbon skeletons for amino acids?
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What is primarily incorporated as nitrogen in protein biosynthesis?
What is primarily incorporated as nitrogen in protein biosynthesis?
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What drives ATP synthesis by ATP synthase in mitochondria?
What drives ATP synthesis by ATP synthase in mitochondria?
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What is the driving force for electron transport through photosystem II in photosynthesis?
What is the driving force for electron transport through photosystem II in photosynthesis?
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What regulates the activity of Aspartate Transcarbamoylase (ATC)?
What regulates the activity of Aspartate Transcarbamoylase (ATC)?
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What is required for the conversion of pyruvate to glucose in gluconeogenesis?
What is required for the conversion of pyruvate to glucose in gluconeogenesis?
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What is the activated form of glucose used in the synthesis of polysaccharides?
What is the activated form of glucose used in the synthesis of polysaccharides?
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What is the primary function of Photosystem II in photosynthesis?
What is the primary function of Photosystem II in photosynthesis?
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What is responsible for catalyzing the addition of nucleotides during DNA synthesis?
What is responsible for catalyzing the addition of nucleotides during DNA synthesis?
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What is the end product of glycolysis?
What is the end product of glycolysis?
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What drives the formation of peptide bonds during protein synthesis?
What drives the formation of peptide bonds during protein synthesis?
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What is the precursor for both purine and pyrimidine nucleotide synthesis?
What is the precursor for both purine and pyrimidine nucleotide synthesis?
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What is the conversion of atmospheric nitrogen (N2) to ammonia (NH3) called?
What is the conversion of atmospheric nitrogen (N2) to ammonia (NH3) called?
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What is the effectiveness of 6-mercaptopurine in cancer treatment attributed to?
What is the effectiveness of 6-mercaptopurine in cancer treatment attributed to?
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What is the regeneration of Ribulose-1,5-bisphosphate (RuBP) in the Calvin cycle dependent on?
What is the regeneration of Ribulose-1,5-bisphosphate (RuBP) in the Calvin cycle dependent on?
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What is required for the synthesis of the non-essential amino acid alanine from pyruvate?
What is required for the synthesis of the non-essential amino acid alanine from pyruvate?
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What is the role of Phosphofructokinase in glycolysis?
What is the role of Phosphofructokinase in glycolysis?
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What is the activated glucose intermediate formed in polysaccharide synthesis?
What is the activated glucose intermediate formed in polysaccharide synthesis?
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What is required for the addition of each two-carbon unit in the biosynthesis of fatty acids?
What is required for the addition of each two-carbon unit in the biosynthesis of fatty acids?
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What is the primary molecule used by cells as a source of metabolic energy?
What is the primary molecule used by cells as a source of metabolic energy?
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How many ATP molecules are produced per molecule of glucose in glycolysis?
How many ATP molecules are produced per molecule of glucose in glycolysis?
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What does the first law of thermodynamics in biological systems exemplify?
What does the first law of thermodynamics in biological systems exemplify?
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What does chemiosmotic coupling in cellular respiration describe?
What does chemiosmotic coupling in cellular respiration describe?
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What does Gibbs free energy in biological systems predict?
What does Gibbs free energy in biological systems predict?
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What is the primary outcome of the citric acid cycle in cellular metabolism?
What is the primary outcome of the citric acid cycle in cellular metabolism?
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What is the process of ATP generation via proton gradients across membranes called?
What is the process of ATP generation via proton gradients across membranes called?
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What is the process in which plants convert light energy into chemical energy through biochemical reactions involving light absorption, electron transport, and ATP generation?
What is the process in which plants convert light energy into chemical energy through biochemical reactions involving light absorption, electron transport, and ATP generation?
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What is the final electron acceptor in the electron transport chain during aerobic respiration?
What is the final electron acceptor in the electron transport chain during aerobic respiration?
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What does the biosynthesis of cell constituents include?
What does the biosynthesis of cell constituents include?
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What is the process that converts light energy into chemical energy in plants?
What is the process that converts light energy into chemical energy in plants?
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What is involved in gluconeogenesis?
What is involved in gluconeogenesis?
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What is the primary molecule used by cells as a source of metabolic energy?
What is the primary molecule used by cells as a source of metabolic energy?
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How many ATP molecules are produced per molecule of glucose in glycolysis?
How many ATP molecules are produced per molecule of glucose in glycolysis?
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What is the primary outcome of the citric acid cycle in cellular metabolism?
What is the primary outcome of the citric acid cycle in cellular metabolism?
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What does the chemiosmotic coupling in cellular respiration describe?
What does the chemiosmotic coupling in cellular respiration describe?
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What process does photosynthesis involve?
What process does photosynthesis involve?
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What is involved in the biosynthesis of cell constituents?
What is involved in the biosynthesis of cell constituents?
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What is the final electron acceptor in the electron transport chain during aerobic respiration?
What is the final electron acceptor in the electron transport chain during aerobic respiration?
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What does the first law of thermodynamics in biological systems exemplify?
What does the first law of thermodynamics in biological systems exemplify?
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What does Gibbs free energy in biological systems predict?
What does Gibbs free energy in biological systems predict?
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What is the process of ATP generation via proton gradients across membranes known as?
What is the process of ATP generation via proton gradients across membranes known as?
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What is the role of oxygen in the electron transport chain during aerobic respiration?
What is the role of oxygen in the electron transport chain during aerobic respiration?
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What is involved in the process of photosynthesis in plants?
What is involved in the process of photosynthesis in plants?
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Study Notes
Biochemistry Review Questions and Answers
- Ribose-5-phosphate serves as the precursor for both purine and pyrimidine nucleotide synthesis, providing the sugar component of the nucleotides.
- Nitrogen fixation is the process that converts atmospheric nitrogen (N2) into ammonia (NH3), which can then be incorporated into organic compounds.
- Fatty acid synthesis primarily occurs in the cytosol of cells.
- The formation of peptide bonds during protein synthesis is energetically driven by the hydrolysis of GTP.
- Antimetabolites like 6-mercaptopurine work by inhibiting nucleic acid synthesis, thereby preventing cancer cell proliferation.
- Pyruvate is the end product of glycolysis, while glucose-6-phosphate and fructose-1,6-bisphosphate are intermediates in glycolysis.
- Rubisco catalyzes the conversion of CO2 to 3-phosphoglycerate in the Calvin cycle.
- Glutamate is directly synthesized from the citric acid cycle intermediate α-ketoglutarate.
- Oxygen is the final electron acceptor in the mitochondrial electron transport chain, forming water.
- In animal cells, glucose is stored in the form of glycogen.
- Fatty acid synthesis primarily occurs in the cytosol of eukaryotic cells, while NADP+ acts as the final electron acceptor in the light reactions of photosynthesis, where it is reduced to NADPH.
Biochemistry Review Questions
- Glycolysis involves the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, catalyzed by Phosphofructokinase.
- Nitrogen fixation is the conversion of atmospheric nitrogen (N2) to ammonia (NH3) in the nitrogen cycle.
- Protein synthesis is driven by the hydrolysis of two GTP molecules for the addition of each amino acid to the growing polypeptide chain.
- The carbon and nitrogen atoms of the purine ring in purine nucleotides are derived from both glucose and amino acids.
- The regeneration of Ribulose-1,5-bisphosphate (RuBP) in the Calvin cycle requires ATP only.
- DNA polymerase is responsible for catalyzing the addition of nucleotides during DNA synthesis.
- 6-mercaptopurine's effectiveness in cancer treatment is due to its ability to inhibit nucleic acid synthesis.
- The synthesis of the non-essential amino acid alanine from pyruvate requires amination, the addition of an amino group.
- Photosystem II's primary function in photosynthesis is to generate a proton gradient for ATP synthesis.
- The conversion of two molecules of pyruvate to one molecule of glucose in gluconeogenesis requires 6 ATP molecules.
- In polysaccharide synthesis, glucose-1-phosphate reacts with UTP to form UDP-glucose, an activated glucose intermediate.
- In the biosynthesis of fatty acids, the addition of each two-carbon unit requires two molecules of NADPH.
Cellular Metabolism and Biochemical Reactions Overview
- Chemiosmotic coupling is the process of ATP generation via proton gradients across membranes, proposed by Peter Mitchell.
- Photosynthesis is the process in which plants convert light energy into chemical energy through biochemical reactions involving light absorption, electron transport, and ATP generation.
- The biosynthesis of cell constituents includes gluconeogenesis, glycolysis, lipid synthesis, protein and amino acid synthesis, and nucleic acid synthesis pathways.
- Key experiments related to cellular metabolism cover the chemiosmotic theory, antimetabolites in cancer therapy, and biological aspects and treatment approaches for AIDS.
- ATP is the primary molecule used by cells as a source of metabolic energy, distinguishing it from glucose, NADH, and pyruvate.
- Glycolysis produces a net gain of 2 ATP molecules per molecule of glucose, not 4, 8, or 36.
- The first law of thermodynamics in biological systems exemplifies the conversion of chemical energy to mechanical energy.
- Chemiosmotic coupling in cellular respiration describes the generation of ATP through proton gradients, not direct ATP synthesis from glucose, electron transfer, or glucose breakdown.
- Gibbs free energy in biological systems predicts the direction of biochemical reactions, not the rate of enzymatic reactions, entropy change, or total energy.
- The primary outcome of the citric acid cycle in cellular metabolism is the production of ATP, NADH, and FADH2, not the synthesis of glucose, conversion of ATP to ADP, or breakdown of amino acids.
- Photosynthesis is the process that converts light energy into chemical energy in plants, not glycolysis, oxidative phosphorylation, or the Krebs cycle.
- Gluconeogenesis involves synthesizing glucose from amino acids and glycerol, not fatty acids.
- Oxygen is the final electron acceptor in the electron transport chain during aerobic respiration, crucial for the generation of water as a byproduct and the continuation of the electron transport chain.
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Description
Test your knowledge of cellular metabolism and biochemical reactions with this quiz. Explore topics such as chemiosmotic coupling, photosynthesis, biosynthesis of cell constituents, key experiments, ATP as a source of metabolic energy, glycolysis, thermodynamics in biological systems, cellular respiration, Gibbs free energy, and the citric acid cycle. This quiz will challenge your understanding of fundamental concepts in cellular metabolism and biochemistry.