Cellular Manipulation Techniques
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Questions and Answers

What is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment?

  • Cellular manipulation
  • Cell differentiation
  • Cell culture (correct)
  • Cell proliferation
  • Which type of cells are sources of bioproducts, biologics, and biopharmaceuticals?

  • Both prokaryotic and eukaryotic cells (correct)
  • Eukaryotic cells only
  • Prokaryotic cells only
  • Viral cells
  • Which of the following is NOT a commonly used bioreactor for scaling up cell growth and biopharmaceutical production?

  • Anaerobic bioreactor (correct)
  • Fixed-bed bioreactor
  • Hollow fiber perfusion bioreactor
  • Continuous stirred-tank reactor
  • What is the last phase in the bacterial life cycle?

    <p>Death phase</p> Signup and view all the answers

    Which phase of the bacterial life cycle involves the bacteria learning to use the available food and turning on appropriate genes for growth?

    <p>Lag phase</p> Signup and view all the answers

    Which phase is characterized by the rapid multiplication of bacteria using up the food?

    <p>Exponential phase</p> Signup and view all the answers

    Which bacterial strains are most used for expressing proteins in Biotechnology?

    <p>E.coli, Bacillus, Microcuccus</p> Signup and view all the answers

    Which system has become the dominant system for the production of recombinant proteins for clinical applications?

    <p>Mammalian cells</p> Signup and view all the answers

    What is a main disadvantage of using mammalian cells for protein production?

    <p>Expensive</p> Signup and view all the answers

    Which mammalian cell lines are commonly used for producing recombinant protein pharmaceuticals?

    <p>CHO cells, NS0 cells, BHK cells</p> Signup and view all the answers

    What is a potential advantage of plant cell culture over the extraction of compounds from field-grown plant material?

    <p>Continuous production system</p> Signup and view all the answers

    What are the common contaminants of cell culture?

    <p>Chemical and biological contaminants</p> Signup and view all the answers

    What do biotech products (biopharmaceuticals) primarily use for producing proteins for therapeutic purposes?

    <p>Different types of cells (expression systems)</p> Signup and view all the answers

    'Bioreactors' and 'mammalian and plant cell cultures' are techniques used for what purpose?

    <p>To allow large-scale production of proteins</p> Signup and view all the answers

    What are some problems associated with plant tissue culture?

    <p>Differentiation and Necrosis</p> Signup and view all the answers

    Which cell culture contaminants cause visible effects on the culture?

    <p>Mycoplasma, yeast, bacteria (endotoxins/pyrogens) or fungus</p> Signup and view all the answers

    What is the main advantage of using bioreactors and cell cultures in biotechnology?

    <p>Large-scale production of proteins</p> Signup and view all the answers

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