Cellular Manipulation Techniques

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What is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment?

Cellular manipulation

Cell differentiation

Cell culture (correct)

Cell proliferation

Which type of cells are sources of bioproducts, biologics, and biopharmaceuticals?

Both prokaryotic and eukaryotic cells (correct)

Eukaryotic cells only

Prokaryotic cells only

Viral cells

Which of the following is NOT a commonly used bioreactor for scaling up cell growth and biopharmaceutical production?

Anaerobic bioreactor (correct)

Fixed-bed bioreactor

Hollow fiber perfusion bioreactor

Continuous stirred-tank reactor

What is the last phase in the bacterial life cycle?

Death phase Signup and view all the answers

Which phase of the bacterial life cycle involves the bacteria learning to use the available food and turning on appropriate genes for growth?

Lag phase Signup and view all the answers

Which phase is characterized by the rapid multiplication of bacteria using up the food?

Exponential phase Signup and view all the answers

Which bacterial strains are most used for expressing proteins in Biotechnology?

E.coli, Bacillus, Microcuccus Signup and view all the answers

Which system has become the dominant system for the production of recombinant proteins for clinical applications?

Mammalian cells Signup and view all the answers

What is a main disadvantage of using mammalian cells for protein production?

Expensive Signup and view all the answers

Which mammalian cell lines are commonly used for producing recombinant protein pharmaceuticals?

CHO cells, NS0 cells, BHK cells Signup and view all the answers

What is a potential advantage of plant cell culture over the extraction of compounds from field-grown plant material?

Continuous production system Signup and view all the answers

What are the common contaminants of cell culture?

Chemical and biological contaminants Signup and view all the answers

What do biotech products (biopharmaceuticals) primarily use for producing proteins for therapeutic purposes?

Different types of cells (expression systems) Signup and view all the answers

'Bioreactors' and 'mammalian and plant cell cultures' are techniques used for what purpose?

To allow large-scale production of proteins Signup and view all the answers

What are some problems associated with plant tissue culture?

Differentiation and Necrosis Signup and view all the answers

Which cell culture contaminants cause visible effects on the culture?

Mycoplasma, yeast, bacteria (endotoxins/pyrogens) or fungus Signup and view all the answers

What is the main advantage of using bioreactors and cell cultures in biotechnology?

Large-scale production of proteins Signup and view all the answers

Cellular Manipulation Techniques

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