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Questions and Answers
___ is the carbon-containing compound produced by glycolysis.
___ is the carbon-containing compound produced by glycolysis.
- CO2
- Acetyl CoA
- Pyruvate (correct)
- Glucose
In the glycolytic pathway, which of the following are allosterically controlled enzymes?
In the glycolytic pathway, which of the following are allosterically controlled enzymes?
- triose phosphate isomerase
- phosphofructokinase-1 (correct)
- enolase
- none of the above
Yeasts use fermentation to synthesize ATP:
Yeasts use fermentation to synthesize ATP:
- because yeasts lack mitochondria.
- to produce lactic acid.
- because fermentation produces more ATP than oxidative phosphorylation.
- in the absence of oxygen. (correct)
In the overall reaction for cellular respiration, glucose is:
In the overall reaction for cellular respiration, glucose is:
Phosphofructokinase is ____ active in the glycolytic pathway when the levels of ATP are high in cell because ____.
Phosphofructokinase is ____ active in the glycolytic pathway when the levels of ATP are high in cell because ____.
In the absence of oxygen, NAD+ is recovered by ____, which leads to a net production of ____ ATP molecules for each glucose molecule broken down.
In the absence of oxygen, NAD+ is recovered by ____, which leads to a net production of ____ ATP molecules for each glucose molecule broken down.
Which of the following is FALSE regarding mitochondrial structure?
Which of the following is FALSE regarding mitochondrial structure?
Which of the following health-related conditions are NOT related to mitochondrial function?
Which of the following health-related conditions are NOT related to mitochondrial function?
Electron transport from NADH and FADH2 to O2 occurs in the:
Electron transport from NADH and FADH2 to O2 occurs in the:
Compared with glucose, oxidation of which of the following is more important in humans as a source of ATP?
Compared with glucose, oxidation of which of the following is more important in humans as a source of ATP?
The four stages of glucose oxidation are listed below. Place them in the correct order.
I. pyruvate oxidation to CO2 in the mitochondrion via a 2-carbon acetyl CoA intermediate (citric acid cycle)
II. electron transport to generate a proton motive force
III. conversion in the cytosol of one 6-carbon glucose molecule to two 3-carbon pyruvate molecules (pyruvate) (glycolysis)
IV. ATP synthesis in the mitochondrion (oxidative phosphorylation)
The four stages of glucose oxidation are listed below. Place them in the correct order. I. pyruvate oxidation to CO2 in the mitochondrion via a 2-carbon acetyl CoA intermediate (citric acid cycle) II. electron transport to generate a proton motive force III. conversion in the cytosol of one 6-carbon glucose molecule to two 3-carbon pyruvate molecules (pyruvate) (glycolysis) IV. ATP synthesis in the mitochondrion (oxidative phosphorylation)
The molecule that immediately enters the citric acid cycle is formed by which process?
The molecule that immediately enters the citric acid cycle is formed by which process?
The first step in the citric acid cycle occurs when acetyl CoA reacts with oxaloacetate to form:
The first step in the citric acid cycle occurs when acetyl CoA reacts with oxaloacetate to form:
Glucose is not the only energy-containing molecule that can enter cellular respiration pathways. Which food source is most likely to enter the citric acid cycle as fatty acids?
Glucose is not the only energy-containing molecule that can enter cellular respiration pathways. Which food source is most likely to enter the citric acid cycle as fatty acids?
NADH-COQ reductase and CoQH2-cytochrome c reductase each use the energy derived from electron transfer to transport ____ the mitochondrial matrix.
NADH-COQ reductase and CoQH2-cytochrome c reductase each use the energy derived from electron transfer to transport ____ the mitochondrial matrix.
___ is a lipid soluble molecule that acts to shuttle electrons within the mitochondrial inner membrane.
___ is a lipid soluble molecule that acts to shuttle electrons within the mitochondrial inner membrane.
In mitochondria, the proton-motive force is due largely to:
In mitochondria, the proton-motive force is due largely to:
During cellular respiration, the electron transport chain utilizes the energy produced from passing electrons from one molecule to the next to create:
During cellular respiration, the electron transport chain utilizes the energy produced from passing electrons from one molecule to the next to create:
Which of the following statement(s) regarding the origin of the mitochondria is(are) TRUE?
Which of the following statement(s) regarding the origin of the mitochondria is(are) TRUE?
During ATP synthesis, protons move “down” their electrochemical gradient through:
During ATP synthesis, protons move “down” their electrochemical gradient through:
Transport of pyruvate into the mitochondrial matrix depends on energy provided by:
Transport of pyruvate into the mitochondrial matrix depends on energy provided by:
Brown-fat mitochondria uncouple oxidative phosphorylation to produce:
Brown-fat mitochondria uncouple oxidative phosphorylation to produce:
A major source of reactive oxygen species (ROS) in animal cells is:
A major source of reactive oxygen species (ROS) in animal cells is:
In chloroplasts, light absorption, electron transport, and ATP synthesis all occur:
In chloroplasts, light absorption, electron transport, and ATP synthesis all occur:
The principal pigment involved in photosynthesis is:
The principal pigment involved in photosynthesis is:
In photosynthesis, all of the following reactions are directly dependent on light, except:
In photosynthesis, all of the following reactions are directly dependent on light, except:
Based on what you know about the action spectra of photosynthesis, irradiating a leaf with which of the following light types would result in the release of the greatest quantities of oxygen?
Based on what you know about the action spectra of photosynthesis, irradiating a leaf with which of the following light types would result in the release of the greatest quantities of oxygen?
The "tail" of chlorophyll is hydrophobic, which is important for:
The "tail" of chlorophyll is hydrophobic, which is important for:
Plastoquinone, like ubiquinone, can move freely within the membrane. This is important for its function as:
Plastoquinone, like ubiquinone, can move freely within the membrane. This is important for its function as:
What is the role of water in the light reactions of photosynthesis?
What is the role of water in the light reactions of photosynthesis?
Early investigators thought the oxygen produced by photosynthetic plants came from carbon dioxide. In fact, it comes from:
Early investigators thought the oxygen produced by photosynthetic plants came from carbon dioxide. In fact, it comes from:
Cyclic electron flow in the thylakoid membrane generates:
Cyclic electron flow in the thylakoid membrane generates:
During photosynthesis, O2 is produced:
During photosynthesis, O2 is produced:
During cyclic electron flow, electron transport:
During cyclic electron flow, electron transport:
All of the following statements describe the process of photorespiration, except:
All of the following statements describe the process of photorespiration, except:
The enzymes that catalyze the Calvin cycle are found in the:
The enzymes that catalyze the Calvin cycle are found in the:
The fixation of CO2 into carbohydrates is catalyzed by:
The fixation of CO2 into carbohydrates is catalyzed by:
Plants use ____ to transport sucrose to all regions of the organism.
Plants use ____ to transport sucrose to all regions of the organism.
Why doesn't the Calvin cycle end after a three-carbon sugar is produced?
Why doesn't the Calvin cycle end after a three-carbon sugar is produced?
The Calvin cycle:
The Calvin cycle:
What is the role of substrate-level phosphorylation in glycolysis?
What is the role of substrate-level phosphorylation in glycolysis?
During prolonged exercise, oxygen is scarce in muscle tissue. Under these conditions, muscle cells convert pyruvate to two molecules of lactic acid. What happens to the lactic acid that is generated in this way?
During prolonged exercise, oxygen is scarce in muscle tissue. Under these conditions, muscle cells convert pyruvate to two molecules of lactic acid. What happens to the lactic acid that is generated in this way?
What is the function of the malate–aspartate shuttle?
What is the function of the malate–aspartate shuttle?
What molecule acts as an electron donor during photosynthesis in chloroplasts? What alternative is used by some photosynthetic bacteria (e.g., purple bacteria)?
What molecule acts as an electron donor during photosynthesis in chloroplasts? What alternative is used by some photosynthetic bacteria (e.g., purple bacteria)?
Which components of PSII are responsible for producing the proton-motive force?
Which components of PSII are responsible for producing the proton-motive force?
ATP synthase is composed of two oligomeric proteins, F0 and F1. What is the function of each protein complex and where is each found in mitochondria?
ATP synthase is composed of two oligomeric proteins, F0 and F1. What is the function of each protein complex and where is each found in mitochondria?
What is the source of the energy for carbon fixation?
What is the source of the energy for carbon fixation?
What is photorespiration? How is it related to photosynthesis?
What is photorespiration? How is it related to photosynthesis?
Explain how the rates of photosynthesis in plants like corn and sugarcane can be two to three times faster than the rates of photosynthesis in plants like wheat or rice.
Explain how the rates of photosynthesis in plants like corn and sugarcane can be two to three times faster than the rates of photosynthesis in plants like wheat or rice.
Which stages of photosynthesis can occur only in the light and which can also occur in the dark?
Which stages of photosynthesis can occur only in the light and which can also occur in the dark?
What is the role of quinone in generating the charge separation needed to remove electrons from H2O for use in electron transport?
What is the role of quinone in generating the charge separation needed to remove electrons from H2O for use in electron transport?
Each cytochrome in the electron transport chain has a different reduction potential. What is the importance of these differences for electron transport?
Each cytochrome in the electron transport chain has a different reduction potential. What is the importance of these differences for electron transport?
Flashcards
Glycolysis product
Glycolysis product
The carbon-containing compound produced in glycolysis is pyruvate.
Allosterically controlled enzymes in glycolysis
Allosterically controlled enzymes in glycolysis
Phosphofructokinase-1 is an allosterically controlled enzyme in glycolysis.
Yeast ATP synthesis
Yeast ATP synthesis
Yeasts use fermentation to produce ATP in the absence of oxygen.
Substrate-level phosphorylation in glycolysis
Substrate-level phosphorylation in glycolysis
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Lactic acid fate
Lactic acid fate
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Cellular respiration glucose oxidation
Cellular respiration glucose oxidation
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Phosphofructokinase activation
Phosphofructokinase activation
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Fermentation NAD+ recovery
Fermentation NAD+ recovery
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Mitochondrial structure - important features
Mitochondrial structure - important features
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Health conditions and mitochondria
Health conditions and mitochondria
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Electron transport location
Electron transport location
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ATP source in humans
ATP source in humans
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Glucose oxidation stages order
Glucose oxidation stages order
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Malate-Aspartate Shuttle Function
Malate-Aspartate Shuttle Function
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Citric acid cycle entry
Citric acid cycle entry
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Citric acid cycle first step
Citric acid cycle first step
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Citric Acid Cycle energy source
Citric Acid Cycle energy source
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Electron transport chain proton transport
Electron transport chain proton transport
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Electron shuttle molecule
Electron shuttle molecule
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Proton-motive force origin
Proton-motive force origin
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Importance of cytochrome reduction potentials
Importance of cytochrome reduction potentials
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Electron transport chain energy use
Electron transport chain energy use
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Mitochondria origin theory
Mitochondria origin theory
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ATP synthesis mechanism
ATP synthesis mechanism
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Pyruvate import energy source
Pyruvate import energy source
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Brown fat mitochondria function
Brown fat mitochondria function
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ROS source in cells
ROS source in cells
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ATP synthase components
ATP synthase components
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Photosynthesis organelle location
Photosynthesis organelle location
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Photosynthesis's main pigment
Photosynthesis's main pigment
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Non-light dependent reaction in photosynthesis
Non-light dependent reaction in photosynthesis
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Photosynthesis water role
Photosynthesis water role
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Calvin cycle location
Calvin cycle location
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CO2 fixation enzyme
CO2 fixation enzyme
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Plant sucrose transport
Plant sucrose transport
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Calvin cycle energy source
Calvin cycle energy source
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Photorespiration effect
Photorespiration effect
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Fast C4 photosynthesis
Fast C4 photosynthesis
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Calvin cycle continuation
Calvin cycle continuation
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Calvin cycle output
Calvin cycle output
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Study Notes
Cellular Energetics
- Glycolysis product: Pyruvate is the carbon-containing compound produced by glycolysis.
- Allosterically controlled enzymes: Phosphofructokinase-1 is among the enzymes in glycolysis that are allosterically controlled.
- Yeast fermentation: Yeasts use fermentation to synthesize ATP in the absence of oxygen.
- Substrate-level phosphorylation: During glycolysis, substrate-level phosphorylation is used to create ATP through transfer of a high-energy phosphate group (from 1,3-bisphosphate or phosphoenolpyruvate) to ADP.
- Lactic acid in prolonged exercise: Muscle cells convert pyruvate to lactic acid when oxygen is scarce during prolonged exercise. The body then either reoxidizes lactic acid back to pyruvic acid and uses it to generate energy or converts it back into glucose.
- Cellular respiration: In cellular respiration, glucose is oxidized to CO2.
- Phosphofructokinase activity and ATP levels: Phosphofructokinase is more active when ATP levels are low in the cell. ATP is an allosteric inhibitor of this enzyme, meaning its activity is affected by the binding of ATP at a site other than the active site.
- NAD+ recovery: In the absence of oxygen, NAD+ is recovered through fermentation, resulting in the net production of two ATP molecules per glucose molecule.
- Mitochondrial structure: The inner mitochondrial membrane has a larger surface area than the outer membrane and contains many proteins needed for ATP generation. Mitochondria contain DNA in the matrix.
- Mitochondrial function: Conditions such as Parkinson's disease, Kearns-Sayre syndrome, and aging are not directly related to mitochondrial function. However, Tay–Sachs disease is.
- Electron transport chain placement: The electron transport chain occurs in the mitochondrial inner membrane.
- ATP production from glucose oxidation: Glucose oxidation is more important in humans as a source of ATP when compared to cellulose, sucrose, and proteins. Fats are a more important source of ATP.
- Stages of glucose oxidation: The four stages of glucose oxidation are:
- pyruvate oxidation to acetyl CoA
- Krebs cycle (citric acid cycle)
- electron transport chain
- oxidative phosphorylation
- Malate-aspartate shuttle function: The malate-aspartate shuttle delivers electrons from glycolysis to the mitochondrial matrix, reducing NAD+ to NADH for use in the electron transport chain.
- Citric acid cycle precursor: Pyruvate conversion is the process that produces the molecule that immediately enters the citric acid cycle.
- Proton-motive force: In mitochondria, the proton-motive force is primarily due to a voltage gradient across the inner membrane.
- Cytochromes in electron transport: Different cytochromes in the electron transport chain have different reduction potentials, driving the unidirectional flow of electrons.
- Electron Transport Chain and Proton Gradient: The electron transport chain uses the energy from passing electrons to create a proton gradient.
- Mitochondria origin: Mitochondria are believed to have originated from a bacterium that established a symbiotic relationship with a eukaryotic host cell. The outer mitochondrial membrane is derived from the bacterial plasma membrane.
Photosynthesis
- Light-dependent reactions: Carbon fixation is not directly dependent on light. Other processes like ATP synthesis, electron transport, and the removal of electrons from water do depend on light directly.
- Photosynthesis stages: The first three stages of photosynthesis (light absorption, electron transport, and ATP production) depend on light. Carbon fixation occurs whether light is present or not.
- Electron donor in photosynthesis: Water is the electron donor in the light reactions of chloroplasts; some bacteria use different electron donors like H2S.
- Photosynthesis and oxygen: The oxygen produced by plants during photosynthesis comes from water, not CO2.
- Chlorophyll's hydrophobic tail: The hydrophobic tail of chlorophyll is important for anchoring chlorophyll in the thylakoid membrane.
- Principal photosynthetic pigment: Chlorophyll a is the principal pigment involved in photosynthesis.
- Electron transport in chloroplasts: Plastoquinone acts as an electron and proton shuttle in chloroplasts.
- Carbon fixation enzyme : RuBP carboxylase(rubisco) catalyzes CO2 fixation.
- Photosynthesis and photorespiration: Photorespiration uses oxygen, competes with photosynthesis and generates CO2.
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