Cellular Energetics and Respiration

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Questions and Answers

What is the primary product generated by the electron transport chain during cellular respiration?

  • Carbon Dioxide
  • Glucose
  • Oxygen
  • ATP (correct)

In the Krebs Cycle, what is the main purpose of acetyl-CoA?

  • To enter the cycle and aid in the production of electron carriers (correct)
  • To produce glucose from carbon dioxide
  • To serve as a substrate for fatty acid synthesis
  • To enhance cellular respiration rates

Which component of the electron transport chain is lipid-soluble and facilitates electron transfer?

  • NADH
  • FADH2
  • CoQ (correct)
  • Cytochrome c

What drives the proton-motive force in mitochondria during cellular respiration?

<p>A voltage gradient across the inner membrane (D)</p> Signup and view all the answers

How do the different reduction potentials of cytochromes affect electron transport?

<p>They allow for a directional flow of electrons. (D)</p> Signup and view all the answers

What is the role of NADH in the Krebs Cycle?

<p>To provide electrons for the electron transport chain (C)</p> Signup and view all the answers

Which of the following statements about mitochondrial diseases is true?

<p>They may cause problems with energy metabolism throughout the body. (C)</p> Signup and view all the answers

During glycolysis, which process occurs in the cytoplasm to generate energy?

<p>Conversion of glucose to pyruvate (C)</p> Signup and view all the answers

What is the primary carbon-containing compound produced during glycolysis?

<p>Pyruvate (B)</p> Signup and view all the answers

Which enzyme in the glycolytic pathway is known to be allosterically regulated?

<p>Phosphofructokinase-1 (C)</p> Signup and view all the answers

Why do yeasts utilize fermentation to generate ATP?

<p>In the absence of oxygen (C)</p> Signup and view all the answers

What is the purpose of substrate-level phosphorylation in glycolysis?

<p>To synthesize ATP (C)</p> Signup and view all the answers

What happens to lactic acid produced during anaerobic respiration in muscles?

<p>It is secreted into the bloodstream (B)</p> Signup and view all the answers

Which of the following statements about the Krebs cycle is correct?

<p>It generates a significant number of NADH and FADH2 (A)</p> Signup and view all the answers

In which cellular structure does the electron transport chain primarily occur?

<p>Inner mitochondrial membrane (C)</p> Signup and view all the answers

Mitochondrial diseases often impair which of the following processes?

<p>Oxidative phosphorylation and ATP production (D)</p> Signup and view all the answers

What is the primary purpose of phosphofructokinase in the glycolytic pathway?

<p>To regulate the breakdown of glucose (A)</p> Signup and view all the answers

What happens to NAD+ during fermentation in the absence of oxygen?

<p>It is recovered by converting pyruvate (C)</p> Signup and view all the answers

Which of the following statements about mitochondrial structure is true?

<p>The cristae increase the surface area for biochemical reactions (C)</p> Signup and view all the answers

Which molecule accepts electrons at the end of the electron transport chain?

<p>O2 (C)</p> Signup and view all the answers

Which health condition is associated with mitochondrial dysfunction?

<p>Parkinson’s disease (C)</p> Signup and view all the answers

What is a key difference between glycolysis and the Krebs Cycle in terms of oxygen requirement?

<p>Both processes can occur without oxygen (B)</p> Signup and view all the answers

Which product is generated by the Krebs Cycle during the breakdown of glucose?

<p>ATP (C)</p> Signup and view all the answers

How many molecules of ATP are produced from glycolysis per glucose molecule?

<p>Two (B)</p> Signup and view all the answers

Flashcards

Cellular Respiration Glucose Oxidation

Glucose is oxidized to carbon dioxide (CO2) in cellular respiration

Phosphofructokinase and ATP

Phosphofructokinase activity is low when intracellular ATP levels are high, ATP acts as an allosteric inhibitor.

Fermentation and NAD+

In the absence of oxygen, fermentation regenerates NAD+ which is crucial for glycolysis, yielding 2 ATP molecules per glucose.

Mitochondrial Inner Membrane

Contains proteins needed for ATP production, and has higher surface area than the outer membrane due to cristae.

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Mitochondrial Function Health Conditions

Conditions like Parkinson's, Kearns-Sayre syndrome and aging are associated with issues in mitochondrial function, but not Tay-Sachs disease.

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Electron Transport Chain Location

Electron transport from NADH and FADH2 to O2 occurs in the mitochondrial inner membrane.

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ATP Production & Oxidation

Oxidizing other molecules, besides glucose, is more important than glucose in humans for ATP generation.

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Mitochondrial Structure: DNA Location

Mitochondria contain DNA within the matrix.

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Mitochondrial proton gradient

A difference in proton concentration across the inner mitochondrial membrane, driving ATP synthesis.

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Electron transport chain cytochromes

Proteins in the inner mitochondrial membrane with differing electron acceptance abilities, driving electron flow.

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Coenzyme Q (CoQ)

Lipid-soluble electron carrier in the mitochondrial inner membrane, shuttling electrons.

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Electron transport chain protons

The electron transport chain uses energy from electron transfer to pump protons across the inner mitochondrial membrane.

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NADH-CoQ reductase

Enzyme in the electron transport chain facilitating electron transfer.

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Proton-motive force

The potential energy stored across the inner mitochondrial membrane through proton gradient.

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Electron flow direction in ETC

Electrons flow from molecules with lower reduction potentials to molecules with higher reduction potentials in the electron transport chain.

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Electron Transport Chain (ETC) purpose

Uses energy from electron transfer to create a proton gradient across the membrane to power ATP synthesis.

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Product of glycolysis

Pyruvate is the carbon-containing compound produced by the process of glycolysis.

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Allosterically controlled enzyme in glycolysis

Phosphofructokinase-1 is an allosterically controlled enzyme in glycolysis.

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Fermentation in Yeast

Yeasts use fermentation to produce ATP in the absence of oxygen.

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Substrate-level phosphorylation in glycolysis

Substrate-level phosphorylation is a way to produce ATP during glycolysis by transferring a high-energy phosphate group to ADP.

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Lactic acid generation during exercise

During prolonged exercise, muscle cells convert pyruvate to lactic acid.

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Fate of lactic acid in the body

Lactic acid is secreted into the bloodstream, then processed by the liver where it can reoxidized to pyruvate, metabolized to generate energy, or converted back to glucose and stored as glycogen.

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Glycolysis

Glycolysis is the metabolic pathway that breaks down glucose into pyruvate.

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ATP production

ATP is the primary energy currency of cells. It is produced in various cellular processes.

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Study Notes

Cellular Energetics

  • Glycolysis produces pyruvate
  • Phosphofructokinase-1 is an allosterically controlled enzyme in glycolysis
  • Fermentation produces ATP in the absence of oxygen
  • Substrate-level phosphorylation transfers a high-energy phosphate group from a molecule to ADP to make ATP

Cellular Respiration

  • Under aerobic conditions, pyruvate is converted to acetyl CoA and enters the citric acid cycle.
  • The complete oxidation of glucose occurs in the citric acid cycle and electron transport chain
  • In the overall reaction for cellular respiration, glucose is oxidized to CO2
  • The molecule that immediately enters the citric acid cycle is formed by pyruvate conversion
  • Phosphofructokinase is less active when ATP levels are high, ATP is an allosteric inhibitor

Mitochondrial Structure

  • Inner mitochondrial membrane has a larger surface area than the outer membrane
  • Inner membrane contains many proteins needed for ATP synthesis
  • Mitochondria contain DNA in the matrix
  • The curved morphology of cristae is due to MICOS protein complexes
  • The outer mitochondrial membrane contacts the cellular cytosol and mitochondrial matrix

Electron Transport Chain

  • Electron transport from NADH and FADH2 to O2 occurs in the mitochondrial inner membrane
  • Oxidation of fats compared to glucose, is more important in humans when it comes to source of ATP
  • Electron transport chain uses energy from electron transfer to transport protons into the mitochondrial intermembrane space

Oxidative Phosphorylation

  • Protons flow down electrochemical gradient through ATP synthase
  • NADH-CoQ reductase and CoQH2-cytochrome c reductase each use electron transfer to actively transport protons from the mitochondrial matrix
  • during electron transport the energy is used to produce ATP

Photosynthesis

  • Light reactions are directly dependent on light, except carbon fixation
  • In plants, light-dependent reactions happen in the thylakoid membranes, while the Calvin cycle happens in the stroma
  • Oxygen is produced on the luminal face of the thylakoid membrane during photosynthesis
  • ATP synthase, which has Fo and F1, is an important component in the production of ATP
  • The major source of reactive oxygen species in animal cells is electron transport in the mitochondria
  • Water is used as an electron donor in chloroplasts
  • Carbon fixation is catalyzed by RuBisCo in the Calvin cycle, which is found in the stroma during photosynthesis.
  • The Calvin cycle uses ATP and NADPH to produce glucose

Other Information

  • The principal pigment in photosynthesis is chlorophyll a
  • Plastoquinone is an electron and proton shuttle that's within the membrane
  • Photorespiration consumes oxygen and produces carbon dioxide which competes with photosynthesis
  • Photorespiration is favored with low CO2 or high O2 conditions

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