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Questions and Answers
What is the primary function of ribosomes in eukaryotic cells?
What is the primary function of ribosomes in eukaryotic cells?
Ribosomes are responsible for protein synthesis.
How are prokaryotic cells structurally different from eukaryotic cells?
How are prokaryotic cells structurally different from eukaryotic cells?
Prokaryotic cells lack a nucleus and membrane-bound organelles.
Explain the importance of the surface area to volume ratio in cells.
Explain the importance of the surface area to volume ratio in cells.
A higher surface area to volume ratio allows for more efficient exchange of materials.
What distinguishes the rough endoplasmic reticulum from the smooth endoplasmic reticulum?
What distinguishes the rough endoplasmic reticulum from the smooth endoplasmic reticulum?
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Describe the structure of the plasma membrane.
Describe the structure of the plasma membrane.
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What role do fluorescent dyes play in microscopy?
What role do fluorescent dyes play in microscopy?
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What are the two basic types of electron microscopes?
What are the two basic types of electron microscopes?
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What is the function of the nucleolus within the nucleus?
What is the function of the nucleolus within the nucleus?
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What is the fluid mosaic model?
What is the fluid mosaic model?
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How does the nuclear envelope contribute to cellular processes?
How does the nuclear envelope contribute to cellular processes?
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What component of the endomembrane system is primarily involved in modifying and packaging proteins?
What component of the endomembrane system is primarily involved in modifying and packaging proteins?
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Why do eukaryotic cells have internal membranes?
Why do eukaryotic cells have internal membranes?
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What is meant by 'magnification' in microscopy?
What is meant by 'magnification' in microscopy?
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Distinguish between the two categories of cells in the three domains of life.
Distinguish between the two categories of cells in the three domains of life.
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What role does the Golgi apparatus play in the modification of glycoproteins?
What role does the Golgi apparatus play in the modification of glycoproteins?
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Describe the primary function of lysosomes in a cell.
Describe the primary function of lysosomes in a cell.
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How do vacuoles function in plant cells?
How do vacuoles function in plant cells?
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What is the significance of the Endosymbiont Theory regarding mitochondria and chloroplasts?
What is the significance of the Endosymbiont Theory regarding mitochondria and chloroplasts?
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Explain the structure and function of microtubules.
Explain the structure and function of microtubules.
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What are the main roles of microfilaments in cellular function?
What are the main roles of microfilaments in cellular function?
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How do plant cell walls differ from animal cell structures?
How do plant cell walls differ from animal cell structures?
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Identify and describe one type of intercellular junction.
Identify and describe one type of intercellular junction.
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What is the function of peroxisomes in the cell?
What is the function of peroxisomes in the cell?
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What is the primary function of the extracellular matrix (ECM) in animal cells?
What is the primary function of the extracellular matrix (ECM) in animal cells?
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How do chloroplasts capture light energy?
How do chloroplasts capture light energy?
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Describe the structure of the cytoskeleton and its main components.
Describe the structure of the cytoskeleton and its main components.
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What is the role of centrosomes in cellular organization?
What is the role of centrosomes in cellular organization?
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How do cilia and flagella contribute to cell movement?
How do cilia and flagella contribute to cell movement?
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Study Notes
Lecture 1: Cell Structure and Function
- All living organisms are composed of cells
- The cell is the basic unit of life
- Cell structure is intricately linked to its function
Microscopy
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Light microscopy (LM) uses visible light to magnify specimens
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Lenses refract light to create a magnified image
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Important parameters include magnification, resolution, and contrast
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Magnification is the ratio of image size to real size
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Resolution is the clarity of the image; lower values mean better resolution (Lower resolution in light microscopy than electron microscopy)
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Contrast is the difference in visible parts of a sample
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Resolution is limited by the wavelength of the radiation used
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Numerical Aperture (NA) describes the ability of a lens to collect light
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Different wavelengths of light provide different levels of resolution
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Various organisms have diverse sizes, from atoms to humans
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Most cells have a size range of 1-100 μm
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Using microscopy helps visualize structures within cells
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Fluorescence uses fluorescent dyes that absorb UV light and emit visible light, allowing scientists to visualize structures
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The fluorescent protein GFP (Green Fluorescent Protein) exhibits bright green fluorescence when exposed to light, making it a very useful tool in biology
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It forms internal chromophores without requiring cofactors or enzymes
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Electron microscopy (EM) provides higher resolution than LM
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Two types exist: Scanning EM (SEM) and Transmission EM (TEM)
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SEM produces 3D images of the specimen's surface
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TEM produces 2D images showing internal structures
Three Domains of Life and the Two Types of Cells
- Life is categorized into three domains: Bacteria, Archaea and Eukarya
- Prokaryotic cells lack internal membrane-bound organelles and a nucleus
- Eukaryotic cells have internal membrane-bound organelles and a nucleus
- Examples of prokaryotic organisms are bacteria and archaea
- Organisms in the domains Protists, Fungi, Plants, and Animals belong to the domain Eukarya
Comparing Prokaryotic and Eukaryotic Cells
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All cells share common features
- Plasma membrane
- Cytosol (Semifluid interior)
- Chromosomes (carry genes; DNA)
- Ribosomes (synthesize proteins)
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Prokaryotic cells have no nucleus or membranous organelles
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Eukaryotic cells have a nucleus and membranous organelles.
The Cytoplasm and Cytosol
- Cytoplasm is the entire region between the nucleus and the plasma membrane.
- Cytosol is the jelly-like substance within the cytoplasm of the cell
Plasma Membrane
- A selective barrier that regulates what enters and leaves the cell
- Composed of a phospholipid bilayer
- Contains embedded proteins.
Fluid Mosaic Model
- Phospholipids are amphipathic molecules—both hydrophobic and hydrophilic.
- Membranes are fluid structures with various proteins embedded within the lipids.
Surface Area to Volume Ratio
- Cell size is constrained by its surface-to-volume ratio.
- Smaller cells have a larger surface area-to-volume ratio than larger cells.
- This is important for efficient exchange of materials.
Increasing Surface Area: Volume Ratio
- Specialized cells and tissues increase their surface area through folds, villi, and microvilli, optimizing material transfer.
A Panoramic View of the Eukaryotic Cell
- Eukaryotic cells contain internal membranes that segregate organelles
- Plants and animal cells share many organelles but have differences
Animal Cell
- Illustrates the internal structures and organelles of an animal cell
Plant Cell
- Illustrates the internal structures and organelles of a plant cell
- The components and processes that are unique to plant cells
The Nucleus
- Houses the cell's DNA (genetic instructions)
- Enclosed by a double membrane (nuclear envelope)
- Pores regulate material transport
- The nuclear lamina provides structural support
- Contains the nucleolus, site of rRNA synthesis.
Ribosomes
- Protein synthesis occurs at ribosomes.
- Ribosomes are found in two locations; free ribosomes in the cytosol or attached to the Endoplasmic Reticulum (ER)
The Endomembrane System
- The endomembrane system regulates protein traffic and performs metabolic functions
- Components include the nuclear envelope, ER, Golgi, lysosomes, vacuoles, and plasma membrane
Endoplasmic Reticulum
- The ER is a network of membranes that is continuous with the nuclear envelope
- Two types of ER exist: Smooth and Rough
- Smooth ER lacks ribosomes, and functions in lipid synthesis and detoxification of poisons
- Rough ER is studded with ribosomes, performing protein synthesis and modification
Golgi Apparatus
- Modifies, sorts, packages, and ships proteins and other macromolecules synthesized in the ER
- Processes and modifies products from the ER and other parts of the cell
Lysosomes
- Digestive compartments containing enzymes that degrade macromolecules
- Catalyze the breakdown of molecules
- Involved in cell's recycling (autophagy)
- Participate in phagocytosis and macromolecule digestion
Vacuoles
- Large sacs used for storage and maintenance
- Plant cells typically have a large central vacuole containing water, salts, proteins, and carbohydrates
Mitochondria
- Site of cellular respiration where chemical energy from the breakdown of sugar is converted into ATP
- Double membrane structure with cristae for increased surface area
Chloroplasts
- Site of photosynthesis where sunlight energy is converted into chemical energy (sugar)
- Contains chlorophyll, a green pigment that absorbs sunlight
Peroxisomes
- Specialized metabolic compartments bounded by a single membrane
- Perform various functions, often involving oxidation
- These functions are connected to other organelles in many cases
Cytoskeleton
- Network of fibers that organizes structures and regulates activities in the cell
- Three types include microtubules, microfilaments, and intermediate filaments
- This complex system provides structure and enables essential functions like cell motility.
Microtubules
- Hollow, cylindrical structures that are part of the cytoskeleton
- Essential during cell division for chromosome separation
- Used in cell motility (cilia and flagella)
Microfilaments (Actin Filaments)
- Important for cell shape, muscle contraction, and intracellular movements (like amoeboid movement)
- Key roles in maintaining cell shape and enabling other specialized cellular processes
Intermediate Filaments
- Provide mechanical support; they help maintain cell shape and coordinate with other organelles
Centrosomes and Centrioles
- Microtubule organizing center
- Plays a crucial role in cell division and the growth of microtubules
Cilia and Flagella
- Locomotor appendages of cells; composed of microtubules arranged in a 9+2 pattern
- Both are motor proteins that drive the bending movements.
Microfilaments (Actin filaments)
- Solid rods built of actin subunits in a twisted double chain
- Helps maintain cell shape and enables various functions, such as muscle contraction and amoeboid movement
Extracellular Components and Connections
- Some cells are surrounded by an extracellular matrix, which helps coordinate activities between neighboring cells
- Cell walls are common in plant and some other eukaryotic cells
Cell Walls of Plants
- Support and maintain shape; made primarily of cellulose
Plasmodesmata (Plant Cells)
- Channels that perforate cell walls to allow for the passage of various molecules
Extracellular Matrix (ECM) of Animal Cells
- A mixture of glycoproteins and fibers that provide structure and support for animal cells
Cell Junctions
- Structures that connect cells to each other within tissues
Tight Junctions, Desmosomes, and Gap Junctions
- Intercellular junctions that join neighboring cells to each other and enable communication
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Description
Test your knowledge on cell structure and its functions with this quiz. Explore concepts related to microscopy, including magnification, resolution, and contrast. Dive into the details of how cells are the fundamental units of life and how microscopy aids in their study.