Cell-Penetrating Peptides in DNA Transformation

Assisting in the penetration of nucleic acids into cells

Small peptide size

Enhancing transformation efforts

<p>Non-covalent interactions</p> Signup and view all the answers

<p>Due to their ability to facilitate DNA penetration into cells</p> Signup and view all the answers

<p>Prolonged reaction times, harsh conditions, toxic reagents, low selectivity, and low yields.</p> Signup and view all the answers

<p>Harnessing the power of genetic engineering in microorganisms through the incorporation of the chalcone synthase (CHS) gene into the microalga Chlamydomonas reinhardtii.</p> Signup and view all the answers

<p>Through biolistic gun, enabling the transfer of the CHS gene alongside a selectable Kanamycin resistance marker.</p> Signup and view all the answers

<p>Revolutionizing plant genetic modification, particularly in crops resistant to conventional methods, enhancing traits like pest resistance and yield.</p> Signup and view all the answers

<p>By enabling precise gene delivery into neuronal cells, advancing research on brain function and disorders.</p> Signup and view all the answers

<p>To transfer the CHS gene into C. reinhardtii for efficient chalcone production.</p> Signup and view all the answers

<p>To induce the phenylpropanoid pathway, thereby increasing the yield of chalcones.</p> Signup and view all the answers

<p>It is responsible for the production of chalcones.</p> Signup and view all the answers

<p>The advantages include being easy to use, fast, and versatile; not limited to host specificity or certain species; possibility of transient and stable expression; and not requiring large amounts of nucleic acids and cells.</p> Signup and view all the answers

<p>C.Reinhardtii is ideal due to its ability to rapidly reproduce, has simple genetics, and the ability to photosynthesize.</p> Signup and view all the answers

<p>The goal is to unlock the potential of C.Reinhardtii as a bio factory for producing valuable secondary metabolites like chalcone.</p> Signup and view all the answers

<p>Biolistic particle delivery avoids using viruses or chemicals which can be potentially toxic.</p> Signup and view all the answers

<p>Large DNA fragments may be transferred or small interfering RNAs during gene silencing.</p> Signup and view all the answers

<p>Chalcone.</p> Signup and view all the answers

<p>Coating DNA onto microscopic carriers and shooting them into target cells at high speed.</p> Signup and view all the answers

<p>It can get DNA through thick tissues, making it useful for transforming plants that are hard to reach.</p> Signup and view all the answers

<p>Two aromatic rings connected by a three-carbon, α, β-unsaturated carbonyl system.</p> Signup and view all the answers

<p>They serve as precursors to several biologically active secondary metabolites.</p> Signup and view all the answers

<p>Modulating metabolic pathways in organisms like Chlamydomonas reinhardtii.</p> Signup and view all the answers

<p>Gene gun.</p> Signup and view all the answers

<p>Polyphenolic compounds.</p> Signup and view all the answers

<p>Several synthetic methods are used for chalcone production.</p> Signup and view all the answers