Cell Membrane Transport Quiz
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Questions and Answers

A transporter protein designed for D-glucose is observed to also bind to D-galactose. What is the most likely effect of this?

  • A structural change in the transporter, preventing it from binding any solute.
  • Enhanced transport of D-glucose due to increased binding sites.
  • Competitive inhibition of D-glucose transport due to the shared binding site. (correct)
  • A change in the transporter's specificity, allowing it to simultaneously transport both.
  • What is the term for the likelihood of an ion channel being open, which directly affects the rate of diffusion through it?

  • Conductance (correct)
  • Gating
  • Selectivity
  • Specificity
  • What type of ion channel responds to changes in the concentration of hormones or neurotransmitters?

  • Ligand-gated channels (correct)
  • Mechanically-gated channels
  • Voltage-gated channels
  • Second-messenger-gated channels
  • If the membrane potential of a cell changes, which type of ion channel would be most likely to respond?

    <p>Voltage-gated channels (A)</p> Signup and view all the answers

    What is the primary mechanism responsible for creating the chemical gradients of ions across the cell membrane?

    <p>Active transport pumps such as the $Na^+-K^+$ ATPase pump (D)</p> Signup and view all the answers

    According to the provided content, what does the variable 'D' represent in the permeability coefficient (P) equation?

    <p>The diffusion coefficient, reflecting the solute particle size and the viscosity of the solution. (A)</p> Signup and view all the answers

    If the membrane thickness (∆X) increases, what would be the effect on the flux (J), assuming all other factors remain constant?

    <p>The flux (J) will decrease. (A)</p> Signup and view all the answers

    In carrier-mediated transport, what does 'Tm' represent?

    <p>The point at which all binding sites are occupied. (C)</p> Signup and view all the answers

    How does an increase in temperature affect the rate of diffusion?

    <p>The rate of diffusion increases. (B)</p> Signup and view all the answers

    What property of carrier proteins allows them to distinguish between D-glucose and L-glucose?

    <p>Stereospecificity. (B)</p> Signup and view all the answers

    What happens to the rate of transport in carrier-mediated transport and with many binding sites available when solute concentration is low?

    <p>The rate of transport increases steeply. (A)</p> Signup and view all the answers

    A substance 'X' is transported via a carrier protein but does not use the same binding site as glucose. What effect would an increased amount of 'X' likely have on glucose transport?

    <p>Glucose transport will remain unaffected. (D)</p> Signup and view all the answers

    According to the permeability equation, which variable represents the difference in concentration between two compartments?

    <p>∆C (C)</p> Signup and view all the answers

    Which of the following best describes the behavior of urea with respect to cell membranes?

    <p>Urea is an ineffective osmole because it freely crosses the membrane, rapidly equilibrating. (D)</p> Signup and view all the answers

    What primarily determines the osmotic pressure and movement of water across a cell membrane?

    <p>The concentration of impermeant solutes. (B)</p> Signup and view all the answers

    A cell is placed in a solution with a higher concentration of impermeant solutes than its intracellular fluid. How would this solution be described?

    <p>Hypertonic, causing the cell to shrink. (A)</p> Signup and view all the answers

    Which of these transport mechanisms directly uses ATP to move substances across the cell membrane?

    <p>Primary active transport (D)</p> Signup and view all the answers

    If the membrane potential is at -70mV, and the equilibrium potential for potassium ions is -94mV, what is the driving force for potassium diffusion?

    <p>24 mV (A)</p> Signup and view all the answers

    Which of the following is a key feature of facilitated diffusion?

    <p>It involves a protein carrier or channel. (C)</p> Signup and view all the answers

    What primarily establishes the negative intracellular charge within a cell?

    <p>Leakage of potassium ions out of the cell via K2P channels. (A)</p> Signup and view all the answers

    What is the main driving force behind the movement of substances in simple diffusion?

    <p>The electrochemical gradient. (D)</p> Signup and view all the answers

    How does the membrane potential (Em) relate to the equilibrium potential of the most permeable ion?

    <p>The Em moves toward the equilibrium potential of the most permeable ion. (B)</p> Signup and view all the answers

    In secondary active transport, what is the source of energy that moves a substance against its concentration gradient?

    <p>The electrochemical gradient set up by primary active transport. (C)</p> Signup and view all the answers

    What is the state when the chemical and electrical forces acting on an ion are in balance.

    <p>Electrochemical equilibrium (A)</p> Signup and view all the answers

    Which of the transport mechanisms does a white blood cell primarily use to engulf bacteria?

    <p>Bulk transport (endocytosis). (A)</p> Signup and view all the answers

    What effect does opening ion channels normally have on intracellular and extracellular ion concentrations?

    <p>It normally has no effect on the concentrations. (B)</p> Signup and view all the answers

    Which receptor type directly mediates changes in membrane potential by altering ion permeability?

    <p>Ligand-gated ion channels (A)</p> Signup and view all the answers

    The activation of a Gq protein-coupled receptor would most directly lead to an increase in which of the following second messengers?

    <p>DAG and IP3 (A)</p> Signup and view all the answers

    Which of the following receptor types primarily influences gene expression?

    <p>Nuclear receptors (C)</p> Signup and view all the answers

    Which of the following signaling molecules directly activates a receptor guanylate cyclase?

    <p>Atrial Natriuretic Peptide (ANP) (B)</p> Signup and view all the answers

    A receptor tyrosine phosphatase, when bound to its ligand, results in which of the following?

    <p>Suppression of enzymatic activity (D)</p> Signup and view all the answers

    What is a key characteristic of a normal action potential for a given cell type?

    <p>It always looks identical, depolarizes to the same potential, and repolarizes to the same resting potential. (B)</p> Signup and view all the answers

    How is the action potential propagated along the axon?

    <p>It is propagated down the axon without decrement, being continuously regenerated. (C)</p> Signup and view all the answers

    An action potential is described as an 'all-or-none' response as it means:

    <p>Only a stimulus over a specific threshold can produce an action potential. (B)</p> Signup and view all the answers

    During the absolute refractory period, why is it impossible to elicit a new action potential even with a very strong stimulus?

    <p>The inactivation gates of the sodium channels are closed. (A)</p> Signup and view all the answers

    Which of these describes the relative refractory period?

    <p>Another action potential can be elicited only with a larger-than-normal stimulus. (A)</p> Signup and view all the answers

    How do local currents contribute to action potential propagation?

    <p>By spreading from active regions to adjacent inactive regions, depolarizing the membrane. (A)</p> Signup and view all the answers

    Where is the action potential typically initiated in a neuron?

    <p>At the initial segment of the axon (axon hillock). (B)</p> Signup and view all the answers

    Which of the following factors influence the conduction velocity of action potentials in nerves?

    <p>Membrane resistance, internal resistance and capacitance. (A)</p> Signup and view all the answers

    Flashcards

    Equilibrium Potential

    The membrane potential at which the chemical gradient driving an ion across the membrane is exactly balanced by the electrical gradient.

    Driving Force

    The difference between the resting membrane potential (RMP) and the equilibrium potential for a specific ion.

    Selective Membrane Permeability

    The cell membrane allows some ions to pass through more easily than others.

    Membrane Potential and Permeability

    The overall membrane potential is influenced by the equilibrium potential of the most permeable ion.

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    Electrical Gradient Generation

    The movement of potassium ions (K+) out of the cell due to the concentration gradient creates a negative charge inside the cell.

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    Channel Conductance

    The ability of a channel to allow ions to flow through it. It depends on the probability that the channel is open. A higher probability of being open means higher conductance or permeability.

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    Diffusion Flux (J)

    The amount of substance that passes through a unit area per unit time.

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    Permeability Coefficient (P)

    The coefficient that describes the ease with which a substance can move through a membrane.

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    Voltage-gated Channels

    These channels open and close in response to changes in voltage across the membrane.

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    Ligand-gated Channels

    These channels are activated by specific molecules binding to them, such as hormones or neurotransmitters.

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    Partition Coefficient (K)

    The ratio of a substance's concentration in the membrane to its concentration in the surrounding solution.

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    Second-messenger-gated Channels

    These channels respond to changes in internal signaling molecules, like cAMP or calcium.

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    Diffusion Coefficient (D)

    The rate at which a substance diffuses through a solution.

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    Mechanically-gated Channels

    These channels are activated by mechanical forces, like stretching or pressure on the membrane.

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    Membrane Thickness (∆X)

    The thickness of the membrane through which diffusion occurs.

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    Concentration Gradient (∆C)

    The difference in concentration of a substance between two compartments.

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    Transport Maximum (Tm)

    The point at which all binding sites on carrier proteins are occupied, resulting in a plateau in transport rate.

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    Stereospecificity

    The specific ability of carrier proteins to bind and transport only certain molecules, usually with specific shapes or structures.

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    What is an ineffective osmole?

    Urea is a molecule that can freely cross cell membranes, meaning it doesn't contribute to osmotic pressure changes.

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    What determines osmotic pressure?

    The osmotic pressure of a solution is determined by the concentration of solutes that cannot cross the cell membrane (impermeable solutes), as these solutes create an uneven water distribution.

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    What is an isotonic solution?

    Isotonic solutions have the same concentration of impermeable solutes as the cell, resulting in no change in cell volume.

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    What is a hypotonic solution?

    Hypotonic solutions have a lower concentration of impermeable solutes than the cell, causing water to move into the cell and potentially cause it to swell or burst.

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    What is a hypertonic solution?

    Hypertonic solutions have a higher concentration of impermeable solutes than the cell, causing water to move out of the cell and potentially cause it to shrink.

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    What is simple diffusion?

    Simple diffusion is the movement of molecules across a membrane from a region of high concentration to a region of low concentration, without the help of any membrane proteins. This process is passive and follows the concentration gradient.

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    What is facilitated diffusion?

    Facilitated diffusion uses a membrane protein to help molecules move across the membrane, following the concentration gradient. This process is also passive.

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    What is active transport?

    Active transport uses energy (ATP) to move molecules across a membrane against their concentration gradient, from a region of low concentration to a region of high concentration.

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    Ligand-gated ion channels

    Receptor class that directly changes membrane permeability by opening or closing ion channels. Examples include GABA, acetylcholine (muscle), ATP, glutamate, and NMDA receptors.

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    G protein-coupled receptors (GPCRs)

    Receptor class that uses G proteins to activate intracellular signaling pathways. Examples include acetylcholine, peptides, cytokines, odorants, and lipids.

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    First messenger

    A signaling molecule that binds to a receptor and initiates a cellular response. Examples include neurotransmitters, hormones, and growth factors.

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    Second messenger

    A signaling molecule produced inside the cell in response to the first messenger. Examples include cAMP, DAG, IP3, and Ca++. They amplify the signal and influence downstream events.

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    Enzyme-linked receptors

    A signaling pathway that involves receptor tyrosine kinases (RTKs) and leads to phosphorylation of intracellular proteins. Examples include insulin, EGF, and erythropoietin receptors.

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    What is meant by the statement that action potentials of a given neuron are identical?

    All action potentials for a given cell look identical, reach the same peak potential during depolarization, and return to the same resting potential after repolarization.

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    How is an action potential propagated down an axon?

    The action potential travels down the entire length of the axon without losing its size or shape. This is because the action potential is regenerated as it moves along the axon.

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    What is meant by the 'all-or-none' principle of action potentials?

    An action potential can only be generated if the stimulus reaches a certain threshold level. Stimuli below this threshold will not trigger an action potential.

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    What is the absolute refractory period?

    This period occurs immediately after an action potential and prevents another action potential from being generated, no matter how strong the stimulus. It is caused by the inactivation of sodium channels during depolarization.

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    What is the relative refractory period?

    This period follows the absolute refractory period. During this time, a stronger than usual stimulus is required to generate an action potential. This is due to the increased potassium conductance, making it harder for the membrane to depolarize.

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    What is conduction velocity?

    The speed at which an action potential travels along a nerve fiber is called conduction velocity. It is determined by factors like the diameter of the axon, the presence of myelination, and the temperature.

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    What is membrane resistance?

    The resistance of the membrane to the flow of ions. A higher membrane resistance means less current leaks out of the axon, allowing the signal to travel further.

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    What is internal resistance?

    The resistance of the cytoplasm to the flow of ions. A lower internal resistance allows for faster conduction.

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    Study Notes

    Homeostasis

    • Homeostasis is a steady state, not equilibrium
    • Homeostasis requires energy
    • Equilibrium does not require energy and is a state where there is no change in a vital parameter
    • A steady state is when a vital parameter is well-regulated
    • The body maintains a constant value for a parameter adjusting actions up or down as needed
    • Negative feedback returns a vital parameter to its stable point
    • Negative feedback maintains homeostasis
    • When a parameter deviates from its stable point, negative feedback is activated
    • Negative feedback leads to a series of changes
    • A stimulus is recognized by a receptor
    • The control center compares input to the reference value (set point)
    • Effectors make adjustments
    • Positive feedback loops amplify a stimulus and accelerate it
    • A positive feedback loop can lead to instability and death

    Electrolyte Content of Body Fluids

    • Electrolytes are measured in mEq/L (milliequivalents per liter)
    • Cations and anions are listed in a table displaying the concentration of various electrolytes in plasma, interstitial fluid, and intracellular fluid
    • Examples of cations are sodium, potassium, calcium, and magnesium
    • Examples of anions are chloride, bicarbonate, sulfate, phosphate, and protein

    Osmotic Pressure and Reflection Coefficient

    • Osmolarity is the concentration of solute particles within a solution and used to determine osmotic pressure
    • Solutes with a reflection coefficient of 0 (σ = 0) move freely across a membrane
    • Determining which solute is permeable is crucial when measuring osmotic pressure
    • Isotonic solutions are always iso-osmotic, but iso-osmotic solutions may not be isotonic; if the reflection coefficient is not zero
    • The concentration of impermeable solute determines osmotic pressure and water movement

    Tonicity of Solutions

    • Isotonic solutions do not change cell volume
    • Hypotonic solutions cause cells to swell
    • Hypertonic solutions cause cells to shrink

    Transport Function of the Plasma Membrane

    • Simple diffusion follows the electrochemical gradient
    • Facilitated diffusion follows the electrochemical gradient with a protein carrier
    • Primary active transport goes against the electrochemical gradient using ATP directly
    • Secondary active transport uses the electrochemical gradient produced by a primary active transport system

    Fick's Law of Diffusion

    • Fick's Law relates the diffusive flux to the concentration gradient across a membrane
    • Rate of diffusion is proportional to the permeability coefficient times the concentration difference divided by the thickness of the membrane
    • Temperature positively correlates with the rate of diffusion

    Saturation in Carrier-Mediated Transport

    • Membrane proteins transport solute molecules
    • Carrier-mediated transport has a maximum transport rate
    • At low concentrations, transport increases proportionally to the concentration of solute until all the transport proteins are occupied.
    • Transport maximum (Tm) is when all transport sites are occupied.
    • The rate of transport becomes constant no matter how high the solute concentration increases

    Stereospecificity in Carrier-Mediated Transport

    • Binding sites are highly specific
    • Stereospecificity means the transported molecule must be the correct isomer
    • The transporter for glucose is highly specific to naturally-occurring D-glucose and doesn't transport its mirror image L-glucose

    Competition in Carrier-Mediated Transport

    • Similar solutes can compete for binding sites
    • A closely related solute might occupy transport sites reducing the availability of sites to transport the primary solute

    Ion Channel Characteristics

    • Conductance (g) is the probability the channel is open, increasing the rate of diffusion
    • Channels are controlled by different sensors such as: Voltage, Ligand, Second-messenger, Mechanically-gated
    • Different types of sensors elicit different responses
    • Ion movement through the channels depends on the relative concentration of ions and the voltage gradient across the membrane

    Mechanisms Responsible for the Resting Membrane Potential

    • Concentration gradients (chemical gradient) are across the membrane due to active transport (pumps)
    • Selective membrane permeability selectively allows potassium (K+) to move across the membrane.
    • Electrical gradients are generated by K+ leak which creates a negative intracellular charge

    Equilibrium Potential

    • The electrical potential difference that exactly balances an ion's concentration gradient across a membrane
    • At equilibrium potential, there is no net flow

    Driving Force for Diffusion: Equilibrium Potential vs. RMP

    • The driving force for an ion is the difference between the membrane potential (RMP) and the equilibrium potential for the ion
    • Forces can be positive or negative

    Characteristics of Action Potentials

    • Stereotypical in size and shape for a given cell type
    • Propagate along a membrane, non-decremental
    • All-or-none response

    Action Potential Sequence

    • Depolarization: Na+ channels open and Na+ enters
    • Repolarization: Na+ channels close and K+ channels open, K+ leaves
    • Hyperpolarization: K+ channels are slow to close and K+ continues to leave.

    Refractory Periods

    • Absolute refractory period: No action potential can be generated no matter the stimulus
    • Relative refractory period: An action potential can be generated but requires a stronger stimulus than usual

    Propagation of Action Potentials

    • Action potentials propagate along a membrane
    • Spread of local currents to adjacent, inactive regions
    • Initiation of Aps in the initial segment of axon

    Factors Affecting Conduction Velocity in Nerves

    • Factors affecting conduction velocities include myelination, axon diameter, temperature.

    Receptors, Signaling Pathways, and Messengers

    • Different types of receptors and how they signal inside a cell.
    • Ligand-gated ion channels, G protein-coupled receptors, enzyme-linked receptors, and nuclear receptors are listed.
    • The signaling pathway for each receptor and the 2nd messengers are listed

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    Description

    Test your understanding of the mechanisms involved in cell membrane transport, including the functioning of transporter proteins and ion channels. This quiz covers key concepts such as permeability coefficients, diffusion rates, and gradients of ions. Enhance your knowledge about how various factors affect these processes.

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