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Questions and Answers
What are the 3 main parts shared by all eukaryotic cells?
What are the 3 main parts shared by all eukaryotic cells?
What are the main components of the cell membrane?
What are the main components of the cell membrane?
Which model describes the structure of the cell membrane?
Which model describes the structure of the cell membrane?
What is the function of the cell membrane?
What is the function of the cell membrane?
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What are the categories of proteins found in the cell membrane?
What are the categories of proteins found in the cell membrane?
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What are the mechanisms of material transport in and out of the cell?
What are the mechanisms of material transport in and out of the cell?
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What is the role of specialized cell membranes in the small intestine?
What is the role of specialized cell membranes in the small intestine?
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What regulates intestinal epithelial permeability?
What regulates intestinal epithelial permeability?
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What is the percentage of lipids in the cell membrane?
What is the percentage of lipids in the cell membrane?
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What is the implication of loss of integrity in tight junctions?
What is the implication of loss of integrity in tight junctions?
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Which molecules mediate cell-to-cell adhesion through various binding mechanisms?
Which molecules mediate cell-to-cell adhesion through various binding mechanisms?
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What is the main function of cell adhesion?
What is the main function of cell adhesion?
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Which component of the cell membrane is affected in diseases like celiac disease?
Which component of the cell membrane is affected in diseases like celiac disease?
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Which type of transport is involved in material transport in and out of the cell?
Which type of transport is involved in material transport in and out of the cell?
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What are the three broad functions of the cytoskeleton?
What are the three broad functions of the cytoskeleton?
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How is the cytoskeleton described in terms of its structure?
How is the cytoskeleton described in terms of its structure?
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Which type of cytoskeletal polymer is most flexible?
Which type of cytoskeletal polymer is most flexible?
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What is a characteristic of intermediate filaments?
What is a characteristic of intermediate filaments?
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Where are type I and type II keratins commonly found?
Where are type I and type II keratins commonly found?
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Which cells form keratin intermediate fibers to resist shear stress?
Which cells form keratin intermediate fibers to resist shear stress?
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What is a common feature of intermediate filaments?
What is a common feature of intermediate filaments?
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What is a characteristic of the cytoskeleton modulation by pathogens?
What is a characteristic of the cytoskeleton modulation by pathogens?
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What is the largest group of intermediate filaments?
What is the largest group of intermediate filaments?
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What are the three main components of the cytoskeleton?
What are the three main components of the cytoskeleton?
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Which protein forms microtubules?
Which protein forms microtubules?
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What are the roles of microtubules in the cell?
What are the roles of microtubules in the cell?
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What are the three main types of microtubules?
What are the three main types of microtubules?
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In which diseases has reduced microtubule stability been observed?
In which diseases has reduced microtubule stability been observed?
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What are microfilaments also known as?
What are microfilaments also known as?
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What biological functions do actin filaments regulate in coordination with microtubules?
What biological functions do actin filaments regulate in coordination with microtubules?
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What are the primary roles of actin filaments?
What are the primary roles of actin filaments?
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What is the function of the tumour suppressor Adenomatous polyposis coli (APC)?
What is the function of the tumour suppressor Adenomatous polyposis coli (APC)?
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What biological processes do microtubules, microfilaments, and intermediate filaments coordinate for?
What biological processes do microtubules, microfilaments, and intermediate filaments coordinate for?
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Which organelle is described as a remnant bacteria?
Which organelle is described as a remnant bacteria?
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What is the size range of mitochondria?
What is the size range of mitochondria?
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Which molecule is described as the energy currency of the cell?
Which molecule is described as the energy currency of the cell?
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What is the specific structure within mitochondria responsible for ATP production?
What is the specific structure within mitochondria responsible for ATP production?
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What is the main process for ATP production?
What is the main process for ATP production?
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Where does glycolysis occur?
Where does glycolysis occur?
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What is the first step of glucose oxidation?
What is the first step of glucose oxidation?
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What role does ATP play in muscle contraction?
What role does ATP play in muscle contraction?
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What is the total number of ATP a typical human turns over per second?
What is the total number of ATP a typical human turns over per second?
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What is the purpose of the breakdown of glucose in cellular respiration?
What is the purpose of the breakdown of glucose in cellular respiration?
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What is the main function of ATP in cells?
What is the main function of ATP in cells?
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How many ATP are produced by substrate-level phosphorylation in glycolysis?
How many ATP are produced by substrate-level phosphorylation in glycolysis?
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What is the primary way for cells to make ATP when oxygen is absent?
What is the primary way for cells to make ATP when oxygen is absent?
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In which organelle does the Krebs cycle occur?
In which organelle does the Krebs cycle occur?
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What is the main function of the rough endoplasmic reticulum?
What is the main function of the rough endoplasmic reticulum?
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Where are proteins modified before being sent to various places in the cell?
Where are proteins modified before being sent to various places in the cell?
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What is the role of the mitochondria in energy production?
What is the role of the mitochondria in energy production?
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What is the consequence of cyanide and carbon monoxide acting on complex IV?
What is the consequence of cyanide and carbon monoxide acting on complex IV?
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What is the main function of the smooth endoplasmic reticulum?
What is the main function of the smooth endoplasmic reticulum?
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What is the role of the Electron transport chain (E.T.C) in mitochondria?
What is the role of the Electron transport chain (E.T.C) in mitochondria?
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What is the consequence of mitochondria using the proton gradient to generate heat?
What is the consequence of mitochondria using the proton gradient to generate heat?
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Which organelle is associated with severe epilepsy, developmental regression, and liver failure due to DNA depletion?
Which organelle is associated with severe epilepsy, developmental regression, and liver failure due to DNA depletion?
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What are the main functions of the Golgi apparatus?
What are the main functions of the Golgi apparatus?
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What is the main function of the endoplasmic reticulum?
What is the main function of the endoplasmic reticulum?
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What is the primary function of the nucleus in a eukaryotic cell?
What is the primary function of the nucleus in a eukaryotic cell?
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Which of the following accurately describes the multifaceted role of DNA within the nucleus?
Which of the following accurately describes the multifaceted role of DNA within the nucleus?
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What percentage of DNA do humans share with each other?
What percentage of DNA do humans share with each other?
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What is the function of the ribosome in a cell?
What is the function of the ribosome in a cell?
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Which accurately identifies the components of nucleotides?
Which accurately identifies the components of nucleotides?
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Identify the correct base pairings among the options provided.
Identify the correct base pairings among the options provided.
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What accurately describes the components involved in the formation of DNA molecules?
What accurately describes the components involved in the formation of DNA molecules?
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Among the options provided, which substances correctly qualify as organic bases?
Among the options provided, which substances correctly qualify as organic bases?
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Replication of DNA occurs
Replication of DNA occurs
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A nucleotide triplet codes for:
A nucleotide triplet codes for:
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Which statement accurately represents the concept known as the 'Central Dogma' of Biology?
Which statement accurately represents the concept known as the 'Central Dogma' of Biology?
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Which statement provides a comprehensive understanding of how the nucleus exerts control over cellular activities?
Which statement provides a comprehensive understanding of how the nucleus exerts control over cellular activities?
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What happens to the mRNA in the cytosol?
What happens to the mRNA in the cytosol?
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Where are proteins modified before being sent to various places in the cell?
Where are proteins modified before being sent to various places in the cell?
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Which nitrogenous base pairs with adenine in DNA?
Which nitrogenous base pairs with adenine in DNA?
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What is the specific structure within the nucleus responsible for packaging DNA into thread-like structures?
What is the specific structure within the nucleus responsible for packaging DNA into thread-like structures?
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How many pairs of chromosomes do human cells typically contain?
How many pairs of chromosomes do human cells typically contain?
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What is the main target of Huntington's disease?
What is the main target of Huntington's disease?
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What is the function of DNA replication?
What is the function of DNA replication?
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What determines the genetic code in DNA?
What determines the genetic code in DNA?
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Which molecule controls the production of enzymes in cells?
Which molecule controls the production of enzymes in cells?
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What is the basic unit of inheritance?
What is the basic unit of inheritance?
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What is the role of DNA in controlling the production of proteins?
What is the role of DNA in controlling the production of proteins?
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What is the specific role of DNA in nerve cells affected by Huntington's disease?
What is the specific role of DNA in nerve cells affected by Huntington's disease?
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What is the primary function of DNA in determining cell structures and functions?
What is the primary function of DNA in determining cell structures and functions?
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What is the main defining characteristic used to initially classify cells into two main classes?
What is the main defining characteristic used to initially classify cells into two main classes?
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What is a distinguishing feature of RNA polymerase in Archaea?
What is a distinguishing feature of RNA polymerase in Archaea?
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What is a characteristic of Archaea's relationship with human bodies?
What is a characteristic of Archaea's relationship with human bodies?
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Why are there no disease-causing archaea?
Why are there no disease-causing archaea?
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In which diseases might archaea be involved in pathogenesis?
In which diseases might archaea be involved in pathogenesis?
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What is a distinguishing feature of tRNA in Archaea?
What is a distinguishing feature of tRNA in Archaea?
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What is a notable difference in the metabolism of Archaea compared to Bacteria?
What is a notable difference in the metabolism of Archaea compared to Bacteria?
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What is a significant characteristic of Archaea's diversity compared to Bacteria?
What is a significant characteristic of Archaea's diversity compared to Bacteria?
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What is responsible for their own division, have their own DNA, and produce enzymes and proteins required for their function?
What is responsible for their own division, have their own DNA, and produce enzymes and proteins required for their function?
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What does the endosymbiotic theory describe?
What does the endosymbiotic theory describe?
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What is evidence for theories on the evolution of living organisms?
What is evidence for theories on the evolution of living organisms?
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What do similarities between eukaryotes and prokaryotes suggest?
What do similarities between eukaryotes and prokaryotes suggest?
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What comprises bacteria and archaea and typically have a diameter of 0.1–5 μm, with circular DNA in a nucleoid?
What comprises bacteria and archaea and typically have a diameter of 0.1–5 μm, with circular DNA in a nucleoid?
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What comprises animals, plants, fungi, and protists, have a diameter of 10-100 μm, and have membrane-bound organelles?
What comprises animals, plants, fungi, and protists, have a diameter of 10-100 μm, and have membrane-bound organelles?
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What come in various shapes, sizes, and arrangements, and can be transparent under a microscope?
What come in various shapes, sizes, and arrangements, and can be transparent under a microscope?
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What are major differences between plants and animals, and between bacteria and archaea?
What are major differences between plants and animals, and between bacteria and archaea?
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What is responsible for the production of oxygen, changing Earth's atmosphere and allowing new oxygen-breathing life forms to emerge?
What is responsible for the production of oxygen, changing Earth's atmosphere and allowing new oxygen-breathing life forms to emerge?
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What is the implication of the similarities between eukaryotes and prokaryotes?
What is the implication of the similarities between eukaryotes and prokaryotes?
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What is responsible for the diversity of life as evidence for theories on the evolution of living organisms?
What is responsible for the diversity of life as evidence for theories on the evolution of living organisms?
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Which statement accurately compares the ribosomes of prokaryotic and eukaryotic cells?
Which statement accurately compares the ribosomes of prokaryotic and eukaryotic cells?
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Which of the following accurately describes the location of ribosomal RNA synthesis in prokaryotic and eukaryotic cells?
Which of the following accurately describes the location of ribosomal RNA synthesis in prokaryotic and eukaryotic cells?
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What distinguishes the size of prokaryotic and eukaryotic ribosomes?
What distinguishes the size of prokaryotic and eukaryotic ribosomes?
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How can the Gram staining process be accurately distinguished between prokaryotes and eukaryotes?
How can the Gram staining process be accurately distinguished between prokaryotes and eukaryotes?
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How does the Gram staining process exploit differences in cell wall structure between Gram-positive and Gram-negative bacteria?
How does the Gram staining process exploit differences in cell wall structure between Gram-positive and Gram-negative bacteria?
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Which statement accurately describes the role of hydrogen bonds in DNA and their influence on complementary base pairing?
Which statement accurately describes the role of hydrogen bonds in DNA and their influence on complementary base pairing?
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In the context of DNA structure, what is the significance of the complementary base pairing phenomenon?
In the context of DNA structure, what is the significance of the complementary base pairing phenomenon?
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How does the concept of minor and major grooves in DNA contribute to the understanding of complementary base pairing?
How does the concept of minor and major grooves in DNA contribute to the understanding of complementary base pairing?
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What is the fate of the newly synthesized polypeptide chain after termination?
What is the fate of the newly synthesized polypeptide chain after termination?
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What event triggers the termination of protein synthesis during translation?
What event triggers the termination of protein synthesis during translation?
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What is the function of transfer RNA (tRNA) during the elongation phase of translation?
What is the function of transfer RNA (tRNA) during the elongation phase of translation?
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What happens during the translocation phase of translation?
What happens during the translocation phase of translation?
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During the initiation phase of translation, what is the role of the initiation factors?
During the initiation phase of translation, what is the role of the initiation factors?
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What is the primary function of ribosomes in the process of protein synthesis?
What is the primary function of ribosomes in the process of protein synthesis?
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What is the primary function of the terminal phosphate group in ATP?
What is the primary function of the terminal phosphate group in ATP?
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Which enzyme is responsible for catalyzing the hydrolysis of ATP?
Which enzyme is responsible for catalyzing the hydrolysis of ATP?
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What is the consequence of ATP hydrolysis in terms of the energy state of the resulting molecules?
What is the consequence of ATP hydrolysis in terms of the energy state of the resulting molecules?
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During which cellular process is ATP directly involved in the phosphorylation of ADP?
During which cellular process is ATP directly involved in the phosphorylation of ADP?
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In what way does the regeneration of ATP contribute to cellular energy balance?
In what way does the regeneration of ATP contribute to cellular energy balance?
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What role do integral proteins play in the fluid mosaic model of the cell membrane?
What role do integral proteins play in the fluid mosaic model of the cell membrane?
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How does the fluid mosaic model explain the distribution of lipids and proteins in the cell membrane?
How does the fluid mosaic model explain the distribution of lipids and proteins in the cell membrane?
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In the context of cytoskeletal filaments, what is the primary mechanism of cellular movement associated with actin filaments, particularly in the process known as treadmilling?
In the context of cytoskeletal filaments, what is the primary mechanism of cellular movement associated with actin filaments, particularly in the process known as treadmilling?
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What distinguishes the dynamics of microtubules from actin filaments in cellular processes?
What distinguishes the dynamics of microtubules from actin filaments in cellular processes?
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What makes dynamic instability a significant event in microtubules, distinguishing it from the treadmilling process in actin filaments?
What makes dynamic instability a significant event in microtubules, distinguishing it from the treadmilling process in actin filaments?
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What is the role of lamellipodia in cell migration, and what molecular process drives their formation at the leading edge?
What is the role of lamellipodia in cell migration, and what molecular process drives their formation at the leading edge?
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During cell migration, what is the function of focal adhesions, and how are they established?
During cell migration, what is the function of focal adhesions, and how are they established?
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What is the significance of actin retrograde flow in the process of cell migration?
What is the significance of actin retrograde flow in the process of cell migration?
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How does the process of rear retraction contribute to the overall cycle of cell migration?
How does the process of rear retraction contribute to the overall cycle of cell migration?
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Study Notes
Cell Membrane Structure and Functions
- The cell membrane is a double-layered membrane composed of lipids (45-50%), proteins (45-50%), and carbohydrates (4-8% - outer surface only).
- It encapsulates all contents of the cell and separates the interior of the cell from the outside environment.
- Lipids in the membrane include phospholipids, cholesterol, and sphingosine-based lipids, while proteins are categorized as integral or peripheral membrane proteins.
- The Fluid Mosaic Model and Lipid Rafts are models that describe the structure of the cell membrane.
- The cell membrane functions as a barrier, regulates transport, reactions, communication with the environment, cell recognition, adhesion, and transport.
- Active transport regulation includes endocytosis (phagocytosis, pinocytosis, receptor-mediated) and exocytosis for material transport in and out of the cell.
- Cell adhesion is essential for communication and coordination among groups of cells, mediated by integrins and other adhesive proteins.
- Cell adhesion molecules (CAMs) include cell-cell adhesion molecules and cell matrix adhesion molecules and mediate cell-to-cell adhesion through various binding mechanisms.
- Specialized cell membranes in the small intestine, affected in diseases like celiac disease, play a crucial role in nutrient absorption and immune response.
- Tight junctions regulate intestinal epithelial permeability, and their loss of integrity can lead to disease implications.
- The text provides an overview of eukaryotic cell structure, plasma membrane models, general functions, specialized cell membranes, and their implications in disease.
- The text also offers multiple-choice questions for testing understanding of cell membrane concepts and provides a contact email for further questions.
The Cytoskeleton and Its Components
- The cytoskeleton is composed of microtubules, microfilaments, and intermediate filaments, which are similar to LEGO pieces that fit together to form larger structures.
- Microtubules form a dense network across the cell, acting as support beams to prevent collapse and are made up of α- and β-tubulin dimers.
- Microtubules can rapidly switch between growing and shrinking states and are critical for mitosis and cell division, forming distinct centrosomes in animal cells.
- There are three main types of microtubules: aster/astal, interpolar, and kinetochore, which serve as extensive highways for transport within the cell and are critical for neuronal survival.
- Reduced microtubule stability has been observed in neurodegenerative diseases such as Alzheimer's, Parkinson's, and motor neuron disease.
- Microfilaments, also known as actin filaments, are smaller and less rigid than microtubules, primarily involved in cell movement and highly dynamic in nature.
- Actin filaments play crucial roles in cell transport, migration, and division, and coordinate with microtubules to regulate biological functions such as focal adhesions.
- Actin filaments are involved in endocytosis, cell division, and cell migration, protruding in response to signals downstream of cell surface receptors.
- The cytoskeleton and its components are essential for various biological processes, including forming contractile rings during cell division and coordinating with microtubules for cell migration.
- Microtubules, microfilaments, and intermediate filaments are the three main components of the cytoskeleton, with microtubules being the largest and strongest structures.
- The cytoskeleton and its components, analogous to LEGO, are assembled into a wide range of structures, disassembled, and reassembled into different shapes, playing critical roles in maintaining cell structure and function.
- The tumour suppressor Adenomatous polyposis coli nucleates actin filaments, and actin filaments are critical in various biological processes, including cell transport, migration, and division.
Cellular Respiration and Protein Synthesis
- The Krebs cycle, also known as the Tricarboxylic acid cycle or Citric acid cycle, was discovered by Hans Krebs in 1937 and occurs in the inner membrane of the mitochondria, generating energy through the oxidation of acetyl CoA.
- Glucose is transferred into ATP through three separate stages: Glycolysis, Krebs cycle, and Electron transport chain (E.T.C).
- The Electron transport chain (E.T.C) comprises a series of protein complexes (complex I to complex IV) and two mobile carriers (Ubiquinone and Cytochrome c), where electrons are passed from NADH to complex I and from FADH2 to complex II, and protons are pumped out into the intermembrane space (IMS).
- Cyanide and carbon monoxide act on complex IV, stopping all electrons from being passed to oxygen, leading to symptoms such as lactic acidosis and a lack of ATP affecting the central nervous system and heart.
- Mitochondria produce energy by using the proton gradient to generate heat, and they can act as miniheaters, producing heat in fat cells through electron flow.
- Mitochondria have a main function in the inner membrane, which is the synthesis of ATP from ADP, pyruvate, and oxygen, resulting in the production of carbon dioxide and water.
- Mitochondrial diseases vary greatly in symptoms, one example being Alpers’ Syndrome, which is associated with severe epilepsy, developmental regression, and liver failure due to mitochondrial DNA depletion caused by mistakes in the POLG gene.
- The endoplasmic reticulum (ER) includes rough and smooth parts, with the rough ER involved in protein synthesis and the smooth ER in lipid synthesis and detoxification of drugs and toxins.
- Proteins destined to be exported from the cell pass through the rough ER and Golgi, where they undergo post-translational modifications before being sent to various places in the cell.
- The Golgi, resembling a stack of pancakes, receives transport vesicles from the rough ER, modifies proteins, and sends them to different cell locations such as endosomes, lysosomes, plasma membrane, and mitochondria.
- Protein synthesis involves mRNA export, ribosome binding, protein production, and secretion signal recognition, followed by delivery to the ER translocon and further processing in the Golgi before reaching their final destinations in the cell.
- The process of cellular respiration and protein synthesis involves complex biochemical pathways and organelles, such as the mitochondria, endoplasmic reticulum, and Golgi, each with specific functions and roles in energy production and cellular protein regulation.
Understanding DNA: Key Facts and Concepts
- DNA, or deoxyribonucleic acid, is present in the nucleus of all living organism cells and controls all chemical changes within cells.
- DNA is a large molecule made up of nucleotides, which consist of a sugar, phosphate group, and nitrogen-containing base.
- The nitrogenous bases in DNA are adenine, thymine, cytosine, and guanine, and they pair up in specific ways (adenine with thymine, and cytosine with guanine).
- DNA replication involves the unwinding and separation of DNA strands, followed by the addition of appropriate nucleotides to create identical double-stranded DNA molecules.
- The sequence of bases in DNA forms the genetic code, with a triplet of bases controlling the production of a particular amino acid in the cell's cytoplasm.
- Each amino acid is coded for by a specific triplet of bases, and the sequence of amino acids determines the protein being produced.
- DNA controls the production of enzymes, which in turn determine the reactions that take place in cells.
- Chromosomes, made of DNA and containing genes, are packaged into thread-like structures called histones in the nucleus of each cell.
- Human cells typically contain 23 pairs of chromosomes, comprising 22 autosomes and the sex chromosomes, X and Y.
- A gene is the basic unit of inheritance, consisting of a sequence of nucleotides, and every person has two copies of each gene inherited from each parent.
- Huntington's disease targets histones through a process called deacetylation, leading to destruction of nerve cells and inhibiting transcription, particularly important in nerve cells that transcribe nerve growth factors.
- DNA and enzymes are crucial in determining cell structures, functions, and reactions, ultimately influencing the nature of the organism.
Evolution and Diversity of Prokaryotes and Eukaryotes
- Photosynthesis led to the production of oxygen, changing Earth's atmosphere and allowing new oxygen-breathing life forms to emerge
- Symbiosis and endosymbiosis describe how different species benefit from living or working together, and how one organism lives inside the other
- Mitochondria and chloroplasts are responsible for their own division, have their own DNA, and produce enzymes and proteins required for their function
- The endosymbiotic theory describes the dependency between a large host cell and ingested bacteria for survival
- The diversity of life is evidence for theories on the evolution of living organisms
- The increase in antibiotic-resistant bacteria and comparison of protein sequences are key factors in understanding the evolution of life
- Similarities between eukaryotes and prokaryotes suggest they originate from the same common ancestor
- Eukaryotes and prokaryotes share some features like cytoplasm, cell membrane, and ribosomes but have evolved independently for a long time
- Prokaryotes comprise bacteria and archaea and typically have a diameter of 0.1–5 μm, with circular DNA in a nucleoid
- Eukaryotes comprise animals, plants, fungi, and protists, have a diameter of 10-100 μm, and have membrane-bound organelles
- Prokaryotes come in various shapes, sizes, and arrangements, and can be transparent under a microscope
- Major differences between plants and animals, and between bacteria and archaea, include the presence of cell walls and different habitats and cell division methods
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